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Chemerin induces endothelial cell inflammation: activation of nuclear factor-kappa beta and monocyte-endothelial adhesion

Chemerin, a chemoattractant protein, acts via a G-protein coupled chemokine receptor, i.e. Chemokine like Receptor 1/ChemR23; levels of which are elevated in pro-inflammatory states such as obesity and type 2 diabetes mellitus (T2DM). Obesity and T2DM patients are at high risk of developing cardiova...

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Autores principales: Dimitriadis, Georgios K., Kaur, Jaspreet, Adya, Raghu, Miras, Alexander D., Mattu, Harman S., Hattersley, John G., Kaltsas, Gregory, Tan, Bee K., Randeva, Harpal S.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Impact Journals LLC 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5908278/
https://www.ncbi.nlm.nih.gov/pubmed/29682177
http://dx.doi.org/10.18632/oncotarget.24659
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author Dimitriadis, Georgios K.
Kaur, Jaspreet
Adya, Raghu
Miras, Alexander D.
Mattu, Harman S.
Hattersley, John G.
Kaltsas, Gregory
Tan, Bee K.
Randeva, Harpal S.
author_facet Dimitriadis, Georgios K.
Kaur, Jaspreet
Adya, Raghu
Miras, Alexander D.
Mattu, Harman S.
Hattersley, John G.
Kaltsas, Gregory
Tan, Bee K.
Randeva, Harpal S.
author_sort Dimitriadis, Georgios K.
collection PubMed
description Chemerin, a chemoattractant protein, acts via a G-protein coupled chemokine receptor, i.e. Chemokine like Receptor 1/ChemR23; levels of which are elevated in pro-inflammatory states such as obesity and type 2 diabetes mellitus (T2DM). Obesity and T2DM patients are at high risk of developing cardiovascular disorders such as atherosclerosis. We have reported that chemerin induces human endothelial cell angiogenesis and since dysregulated angiogenesis and endothelial dysfunction are hallmarks of vascular disease; we sought to determine the effects of chemerin on monocyte-endothelial adhesion, and nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB), a critical pro-inflammatory transcription factor. Human endothelial cells were transfected with pNF-kappaB-Luc plasmid. Chemerin induced NF-κB activation via the MAPK and PI3K/Akt pathways. Western blot analyses and monocyte-endothelial adhesion assay showed that chemerin increased endothelial cell adhesion molecule expression and secretion, namely E-selectin (Endothelial Selectin), VCAM-1 (Vascular Cell Adhesion Molecule-1) and ICAM-1 (Intracellular Adhesion Molecule-1), leading to enhancement of monocyte-endothelial adhesion. Additionally, we showed a synergistic response of the pro-inflammatory mediator, Interleukin-1β with chemerin induced effects. Chemerin plays an important role in endothelial inflammation, as it induces monocyte-endothelial adhesion, a critical step in the development of atherosclerosis.
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spelling pubmed-59082782018-04-20 Chemerin induces endothelial cell inflammation: activation of nuclear factor-kappa beta and monocyte-endothelial adhesion Dimitriadis, Georgios K. Kaur, Jaspreet Adya, Raghu Miras, Alexander D. Mattu, Harman S. Hattersley, John G. Kaltsas, Gregory Tan, Bee K. Randeva, Harpal S. Oncotarget Research Paper Chemerin, a chemoattractant protein, acts via a G-protein coupled chemokine receptor, i.e. Chemokine like Receptor 1/ChemR23; levels of which are elevated in pro-inflammatory states such as obesity and type 2 diabetes mellitus (T2DM). Obesity and T2DM patients are at high risk of developing cardiovascular disorders such as atherosclerosis. We have reported that chemerin induces human endothelial cell angiogenesis and since dysregulated angiogenesis and endothelial dysfunction are hallmarks of vascular disease; we sought to determine the effects of chemerin on monocyte-endothelial adhesion, and nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB), a critical pro-inflammatory transcription factor. Human endothelial cells were transfected with pNF-kappaB-Luc plasmid. Chemerin induced NF-κB activation via the MAPK and PI3K/Akt pathways. Western blot analyses and monocyte-endothelial adhesion assay showed that chemerin increased endothelial cell adhesion molecule expression and secretion, namely E-selectin (Endothelial Selectin), VCAM-1 (Vascular Cell Adhesion Molecule-1) and ICAM-1 (Intracellular Adhesion Molecule-1), leading to enhancement of monocyte-endothelial adhesion. Additionally, we showed a synergistic response of the pro-inflammatory mediator, Interleukin-1β with chemerin induced effects. Chemerin plays an important role in endothelial inflammation, as it induces monocyte-endothelial adhesion, a critical step in the development of atherosclerosis. Impact Journals LLC 2018-03-30 /pmc/articles/PMC5908278/ /pubmed/29682177 http://dx.doi.org/10.18632/oncotarget.24659 Text en Copyright: © 2018 Dimitriadis et al. http://creativecommons.org/licenses/by/3.0/ This article is distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0/) (CC-BY), which permits unrestricted use and redistribution provided that the original author and source are credited.
spellingShingle Research Paper
Dimitriadis, Georgios K.
Kaur, Jaspreet
Adya, Raghu
Miras, Alexander D.
Mattu, Harman S.
Hattersley, John G.
Kaltsas, Gregory
Tan, Bee K.
Randeva, Harpal S.
Chemerin induces endothelial cell inflammation: activation of nuclear factor-kappa beta and monocyte-endothelial adhesion
title Chemerin induces endothelial cell inflammation: activation of nuclear factor-kappa beta and monocyte-endothelial adhesion
title_full Chemerin induces endothelial cell inflammation: activation of nuclear factor-kappa beta and monocyte-endothelial adhesion
title_fullStr Chemerin induces endothelial cell inflammation: activation of nuclear factor-kappa beta and monocyte-endothelial adhesion
title_full_unstemmed Chemerin induces endothelial cell inflammation: activation of nuclear factor-kappa beta and monocyte-endothelial adhesion
title_short Chemerin induces endothelial cell inflammation: activation of nuclear factor-kappa beta and monocyte-endothelial adhesion
title_sort chemerin induces endothelial cell inflammation: activation of nuclear factor-kappa beta and monocyte-endothelial adhesion
topic Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5908278/
https://www.ncbi.nlm.nih.gov/pubmed/29682177
http://dx.doi.org/10.18632/oncotarget.24659
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