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3D Fabrication with Integration Molding of a Graphene Oxide/Polycaprolactone Nanoscaffold for Neurite Regeneration and Angiogenesis
Treating peripheral nerve injury faces major challenges and may benefit from bioactive scaffolds due to the limited autograft resources. Graphene oxide (GO) has emerged as a promising nanomaterial with excellent physical and chemical properties. GO has functional groups that confer biocompatibility...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
John Wiley and Sons Inc.
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5908351/ https://www.ncbi.nlm.nih.gov/pubmed/29721407 http://dx.doi.org/10.1002/advs.201700499 |
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author | Qian, Yun Song, Jialin Zhao, Xiaotian Chen, Wei Ouyang, Yuanming Yuan, Weien Fan, Cunyi |
author_facet | Qian, Yun Song, Jialin Zhao, Xiaotian Chen, Wei Ouyang, Yuanming Yuan, Weien Fan, Cunyi |
author_sort | Qian, Yun |
collection | PubMed |
description | Treating peripheral nerve injury faces major challenges and may benefit from bioactive scaffolds due to the limited autograft resources. Graphene oxide (GO) has emerged as a promising nanomaterial with excellent physical and chemical properties. GO has functional groups that confer biocompatibility that is better than that of graphene. Here, GO/polycaprolactone (PCL) nanoscaffolds are fabricated using an integration molding method. The nanoscaffolds exhibit many merits, including even GO nanoparticle distribution, macroporous structure, and strong mechanical support. Additionally, the process enables excellent quality control. In vitro studies confirm the advantages of the GO/PCL nanoscaffolds in terms of Schwann cell proliferation, viability, and attachment, as well as neural characteristics maintenance. This is the first study to evaluate the in vivo performance of GO‐based nanoscaffolds in this context. GO release and PCL biodegradation is analyzed after long‐term in vivo study. It is also found that the GO/PCL nerve guidance conduit could successfully repair a 15 mm sciatic nerve defect. The pro‐angiogenic characteristic of GO is evaluated in vivo using immunohistochemistry. In addition, the AKT‐endothelial nitric oxide synthase (eNOS)‐vascular endothelial growth factor (VEGF) signaling pathway might play a major role in the angiogenic process. These findings demonstrate that the GO/PCL nanoscaffold efficiently promotes functional and morphological recovery in peripheral nerve regeneration, indicating its promise for tissue engineering applications. |
format | Online Article Text |
id | pubmed-5908351 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | John Wiley and Sons Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-59083512018-05-02 3D Fabrication with Integration Molding of a Graphene Oxide/Polycaprolactone Nanoscaffold for Neurite Regeneration and Angiogenesis Qian, Yun Song, Jialin Zhao, Xiaotian Chen, Wei Ouyang, Yuanming Yuan, Weien Fan, Cunyi Adv Sci (Weinh) Full Papers Treating peripheral nerve injury faces major challenges and may benefit from bioactive scaffolds due to the limited autograft resources. Graphene oxide (GO) has emerged as a promising nanomaterial with excellent physical and chemical properties. GO has functional groups that confer biocompatibility that is better than that of graphene. Here, GO/polycaprolactone (PCL) nanoscaffolds are fabricated using an integration molding method. The nanoscaffolds exhibit many merits, including even GO nanoparticle distribution, macroporous structure, and strong mechanical support. Additionally, the process enables excellent quality control. In vitro studies confirm the advantages of the GO/PCL nanoscaffolds in terms of Schwann cell proliferation, viability, and attachment, as well as neural characteristics maintenance. This is the first study to evaluate the in vivo performance of GO‐based nanoscaffolds in this context. GO release and PCL biodegradation is analyzed after long‐term in vivo study. It is also found that the GO/PCL nerve guidance conduit could successfully repair a 15 mm sciatic nerve defect. The pro‐angiogenic characteristic of GO is evaluated in vivo using immunohistochemistry. In addition, the AKT‐endothelial nitric oxide synthase (eNOS)‐vascular endothelial growth factor (VEGF) signaling pathway might play a major role in the angiogenic process. These findings demonstrate that the GO/PCL nanoscaffold efficiently promotes functional and morphological recovery in peripheral nerve regeneration, indicating its promise for tissue engineering applications. John Wiley and Sons Inc. 2018-01-26 /pmc/articles/PMC5908351/ /pubmed/29721407 http://dx.doi.org/10.1002/advs.201700499 Text en © 2018 The Authors. Published by WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Full Papers Qian, Yun Song, Jialin Zhao, Xiaotian Chen, Wei Ouyang, Yuanming Yuan, Weien Fan, Cunyi 3D Fabrication with Integration Molding of a Graphene Oxide/Polycaprolactone Nanoscaffold for Neurite Regeneration and Angiogenesis |
title | 3D Fabrication with Integration Molding of a Graphene Oxide/Polycaprolactone Nanoscaffold for Neurite Regeneration and Angiogenesis |
title_full | 3D Fabrication with Integration Molding of a Graphene Oxide/Polycaprolactone Nanoscaffold for Neurite Regeneration and Angiogenesis |
title_fullStr | 3D Fabrication with Integration Molding of a Graphene Oxide/Polycaprolactone Nanoscaffold for Neurite Regeneration and Angiogenesis |
title_full_unstemmed | 3D Fabrication with Integration Molding of a Graphene Oxide/Polycaprolactone Nanoscaffold for Neurite Regeneration and Angiogenesis |
title_short | 3D Fabrication with Integration Molding of a Graphene Oxide/Polycaprolactone Nanoscaffold for Neurite Regeneration and Angiogenesis |
title_sort | 3d fabrication with integration molding of a graphene oxide/polycaprolactone nanoscaffold for neurite regeneration and angiogenesis |
topic | Full Papers |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5908351/ https://www.ncbi.nlm.nih.gov/pubmed/29721407 http://dx.doi.org/10.1002/advs.201700499 |
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