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A Micropatterned Multielectrode Shell for 3D Spatiotemporal Recording from Live Cells

Microelectrode arrays (MEAs) have proved to be useful tools for characterizing electrically active cells such as cardiomyocytes and neurons. While there exist a number of integrated electronic chips for recording from small populations or even single cells, they rely primarily on the interface betwe...

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Autores principales: Cools, Jordi, Jin, Qianru, Yoon, Eugene, Alba Burbano, Diego, Luo, Zhenxiang, Cuypers, Dieter, Callewaert, Geert, Braeken, Dries, Gracias, David H.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5908352/
https://www.ncbi.nlm.nih.gov/pubmed/29721420
http://dx.doi.org/10.1002/advs.201700731
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author Cools, Jordi
Jin, Qianru
Yoon, Eugene
Alba Burbano, Diego
Luo, Zhenxiang
Cuypers, Dieter
Callewaert, Geert
Braeken, Dries
Gracias, David H.
author_facet Cools, Jordi
Jin, Qianru
Yoon, Eugene
Alba Burbano, Diego
Luo, Zhenxiang
Cuypers, Dieter
Callewaert, Geert
Braeken, Dries
Gracias, David H.
author_sort Cools, Jordi
collection PubMed
description Microelectrode arrays (MEAs) have proved to be useful tools for characterizing electrically active cells such as cardiomyocytes and neurons. While there exist a number of integrated electronic chips for recording from small populations or even single cells, they rely primarily on the interface between the cells and 2D flat electrodes. Here, an approach that utilizes residual stress‐based self‐folding to create individually addressable multielectrode interfaces that wrap around the cell in 3D and function as an electrical shell‐like recording device is described. These devices are optically transparent, allowing for simultaneous fluorescence imaging. Cell viability is maintained during and after electrode wrapping around the cel and chemicals can diffuse into and out of the self‐folding devices. It is further shown that 3D spatiotemporal recordings are possible and that the action potentials recorded from cultured neonatal rat ventricular cardiomyocytes display significantly higher signal‐to‐noise ratios in comparison with signals recorded with planar extracellular electrodes. It is anticipated that this device can provide the foundation for the development of new‐generation MEAs where dynamic electrode–cell interfacing and recording substitutes the traditional method using static electrodes.
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spelling pubmed-59083522018-05-02 A Micropatterned Multielectrode Shell for 3D Spatiotemporal Recording from Live Cells Cools, Jordi Jin, Qianru Yoon, Eugene Alba Burbano, Diego Luo, Zhenxiang Cuypers, Dieter Callewaert, Geert Braeken, Dries Gracias, David H. Adv Sci (Weinh) Full Papers Microelectrode arrays (MEAs) have proved to be useful tools for characterizing electrically active cells such as cardiomyocytes and neurons. While there exist a number of integrated electronic chips for recording from small populations or even single cells, they rely primarily on the interface between the cells and 2D flat electrodes. Here, an approach that utilizes residual stress‐based self‐folding to create individually addressable multielectrode interfaces that wrap around the cell in 3D and function as an electrical shell‐like recording device is described. These devices are optically transparent, allowing for simultaneous fluorescence imaging. Cell viability is maintained during and after electrode wrapping around the cel and chemicals can diffuse into and out of the self‐folding devices. It is further shown that 3D spatiotemporal recordings are possible and that the action potentials recorded from cultured neonatal rat ventricular cardiomyocytes display significantly higher signal‐to‐noise ratios in comparison with signals recorded with planar extracellular electrodes. It is anticipated that this device can provide the foundation for the development of new‐generation MEAs where dynamic electrode–cell interfacing and recording substitutes the traditional method using static electrodes. John Wiley and Sons Inc. 2018-01-04 /pmc/articles/PMC5908352/ /pubmed/29721420 http://dx.doi.org/10.1002/advs.201700731 Text en © 2018 The Authors. Published by WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle Full Papers
Cools, Jordi
Jin, Qianru
Yoon, Eugene
Alba Burbano, Diego
Luo, Zhenxiang
Cuypers, Dieter
Callewaert, Geert
Braeken, Dries
Gracias, David H.
A Micropatterned Multielectrode Shell for 3D Spatiotemporal Recording from Live Cells
title A Micropatterned Multielectrode Shell for 3D Spatiotemporal Recording from Live Cells
title_full A Micropatterned Multielectrode Shell for 3D Spatiotemporal Recording from Live Cells
title_fullStr A Micropatterned Multielectrode Shell for 3D Spatiotemporal Recording from Live Cells
title_full_unstemmed A Micropatterned Multielectrode Shell for 3D Spatiotemporal Recording from Live Cells
title_short A Micropatterned Multielectrode Shell for 3D Spatiotemporal Recording from Live Cells
title_sort micropatterned multielectrode shell for 3d spatiotemporal recording from live cells
topic Full Papers
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5908352/
https://www.ncbi.nlm.nih.gov/pubmed/29721420
http://dx.doi.org/10.1002/advs.201700731
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