Cargando…

Resurrection of Dormant Daphnia magna: Protocol and Applications

Long-term studies enable the identification of eco-evolutionary processes that occur over extended time periods. In addition, they provide key empirical data that may be used in predictive modelling to forecast evolutionary responses of natural ecosystems to future environmental changes. However, ex...

Descripción completa

Detalles Bibliográficos
Autores principales: Cuenca Cambronero, Maria, Orsini, Luisa
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MyJove Corporation 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5908671/
https://www.ncbi.nlm.nih.gov/pubmed/29443016
http://dx.doi.org/10.3791/56637
_version_ 1783315753891528704
author Cuenca Cambronero, Maria
Orsini, Luisa
author_facet Cuenca Cambronero, Maria
Orsini, Luisa
author_sort Cuenca Cambronero, Maria
collection PubMed
description Long-term studies enable the identification of eco-evolutionary processes that occur over extended time periods. In addition, they provide key empirical data that may be used in predictive modelling to forecast evolutionary responses of natural ecosystems to future environmental changes. However, excluding a few exceptional cases, long-term studies are scarce because of logistic difficulties associated with accessing temporal samples. Temporal dynamics are frequently studied in the laboratory or in controlled mesocosm experiments with exceptional studies that reconstruct the evolution of natural populations in the wild. Here, a standard operating procedure (SOP) is provided to revive or resurrect dormant Daphnia magna, a widespread zooplankton keystone species in aquatic ecosystems, to dramatically advance the state-of-the-art longitudinal data collection in natural systems. The field of Resurrection Ecology was defined in 1999 by Kerfoot and co-workers, even though the first attempts at hatching diapausing zooplankton eggs date back to the late 1980s. Since Kerfoot's seminal paper, the methodology of resurrecting zooplankton species has been increasingly frequently applied, though propagated among laboratories only via direct knowledge transfer. Here, an SOP is described that provides a step-by-step protocol on the practice of resurrecting dormant Daphnia magna eggs. Two key studies are provided in which the fitness response of resurrected Daphnia magna populations to warming is measured, capitalizing on the ability to study historical and modern populations in the same settings. Finally, the application of next generation sequencing technologies to revived or still dormant stages is discussed. These technologies provide unprecedented power in dissecting the processes and mechanisms of evolution if applied to populations that have experienced changes in selection pressure over time.
format Online
Article
Text
id pubmed-5908671
institution National Center for Biotechnology Information
language English
publishDate 2018
publisher MyJove Corporation
record_format MEDLINE/PubMed
spelling pubmed-59086712018-05-09 Resurrection of Dormant Daphnia magna: Protocol and Applications Cuenca Cambronero, Maria Orsini, Luisa J Vis Exp Environmental Sciences Long-term studies enable the identification of eco-evolutionary processes that occur over extended time periods. In addition, they provide key empirical data that may be used in predictive modelling to forecast evolutionary responses of natural ecosystems to future environmental changes. However, excluding a few exceptional cases, long-term studies are scarce because of logistic difficulties associated with accessing temporal samples. Temporal dynamics are frequently studied in the laboratory or in controlled mesocosm experiments with exceptional studies that reconstruct the evolution of natural populations in the wild. Here, a standard operating procedure (SOP) is provided to revive or resurrect dormant Daphnia magna, a widespread zooplankton keystone species in aquatic ecosystems, to dramatically advance the state-of-the-art longitudinal data collection in natural systems. The field of Resurrection Ecology was defined in 1999 by Kerfoot and co-workers, even though the first attempts at hatching diapausing zooplankton eggs date back to the late 1980s. Since Kerfoot's seminal paper, the methodology of resurrecting zooplankton species has been increasingly frequently applied, though propagated among laboratories only via direct knowledge transfer. Here, an SOP is described that provides a step-by-step protocol on the practice of resurrecting dormant Daphnia magna eggs. Two key studies are provided in which the fitness response of resurrected Daphnia magna populations to warming is measured, capitalizing on the ability to study historical and modern populations in the same settings. Finally, the application of next generation sequencing technologies to revived or still dormant stages is discussed. These technologies provide unprecedented power in dissecting the processes and mechanisms of evolution if applied to populations that have experienced changes in selection pressure over time. MyJove Corporation 2018-01-19 /pmc/articles/PMC5908671/ /pubmed/29443016 http://dx.doi.org/10.3791/56637 Text en Copyright © 2018, Journal of Visualized Experiments http://creativecommons.org/licenses/by/3.0/us/ This is an open-access article distributed under the terms of the Creative Commons Attribution 3.0 License. To view a copy of this license, visithttp://creativecommons.org/licenses/by/3.0/us/
spellingShingle Environmental Sciences
Cuenca Cambronero, Maria
Orsini, Luisa
Resurrection of Dormant Daphnia magna: Protocol and Applications
title Resurrection of Dormant Daphnia magna: Protocol and Applications
title_full Resurrection of Dormant Daphnia magna: Protocol and Applications
title_fullStr Resurrection of Dormant Daphnia magna: Protocol and Applications
title_full_unstemmed Resurrection of Dormant Daphnia magna: Protocol and Applications
title_short Resurrection of Dormant Daphnia magna: Protocol and Applications
title_sort resurrection of dormant daphnia magna: protocol and applications
topic Environmental Sciences
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5908671/
https://www.ncbi.nlm.nih.gov/pubmed/29443016
http://dx.doi.org/10.3791/56637
work_keys_str_mv AT cuencacambroneromaria resurrectionofdormantdaphniamagnaprotocolandapplications
AT orsiniluisa resurrectionofdormantdaphniamagnaprotocolandapplications