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Emergence of Vancomycin-Resistant Enterococcus faecium at an Australian Hospital: A Whole Genome Sequencing Analysis

In 2015, a marked increase in vancomycin-resistant Enterococcus faecium (VREfm) isolation was detected at the Royal Hobart Hospital, Australia. The primary objective of this work was to examine the dynamics of VREfm transmission using whole genome data mapped to public health surveillance informatio...

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Autores principales: Leong, Kelvin W. C., Cooley, Louise A., Anderson, Tara L., Gautam, Sanjay S., McEwan, Belinda, Wells, Anne, Wilson, Fiona, Hughson, Lucy, O’Toole, Ronan F.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5908837/
https://www.ncbi.nlm.nih.gov/pubmed/29674657
http://dx.doi.org/10.1038/s41598-018-24614-6
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author Leong, Kelvin W. C.
Cooley, Louise A.
Anderson, Tara L.
Gautam, Sanjay S.
McEwan, Belinda
Wells, Anne
Wilson, Fiona
Hughson, Lucy
O’Toole, Ronan F.
author_facet Leong, Kelvin W. C.
Cooley, Louise A.
Anderson, Tara L.
Gautam, Sanjay S.
McEwan, Belinda
Wells, Anne
Wilson, Fiona
Hughson, Lucy
O’Toole, Ronan F.
author_sort Leong, Kelvin W. C.
collection PubMed
description In 2015, a marked increase in vancomycin-resistant Enterococcus faecium (VREfm) isolation was detected at the Royal Hobart Hospital, Australia. The primary objective of this work was to examine the dynamics of VREfm transmission using whole genome data mapped to public health surveillance information. Screening and clinical isolates of VREfm from patients were typed for the specific vancomycin-resistance locus present. Of total isolates collected from 2014–2016 (n = 222), 15.3% and 84.7% harboured either the vanA or the vanB vancomycin-resistance locus, respectively. Whole-genome sequencing of 80 isolates was performed in conjunction with single-nucleotide polymorphic (SNP) analysis and in silico multi-locus sequence typing (MLST). Among the isolates sequenced, 5 phylogenetic clades were identified. The largest vanB clade belonged to MLST sequence type ST796 and contained clinical isolates from VREfm infections that clustered closely with isolates from colonised patients. Correlation of VREfm genotypes with spatio-temporal patient movements detected potential points of transmission within the hospital. ST80 emerged as the major vanA sequence type for which the most likely index case of a patient cluster was ascertained from SNP analyses. This work has identified the dominant clones associated with increased VREfm prevalence in a healthcare setting, and their likely direction of transmission.
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spelling pubmed-59088372018-04-30 Emergence of Vancomycin-Resistant Enterococcus faecium at an Australian Hospital: A Whole Genome Sequencing Analysis Leong, Kelvin W. C. Cooley, Louise A. Anderson, Tara L. Gautam, Sanjay S. McEwan, Belinda Wells, Anne Wilson, Fiona Hughson, Lucy O’Toole, Ronan F. Sci Rep Article In 2015, a marked increase in vancomycin-resistant Enterococcus faecium (VREfm) isolation was detected at the Royal Hobart Hospital, Australia. The primary objective of this work was to examine the dynamics of VREfm transmission using whole genome data mapped to public health surveillance information. Screening and clinical isolates of VREfm from patients were typed for the specific vancomycin-resistance locus present. Of total isolates collected from 2014–2016 (n = 222), 15.3% and 84.7% harboured either the vanA or the vanB vancomycin-resistance locus, respectively. Whole-genome sequencing of 80 isolates was performed in conjunction with single-nucleotide polymorphic (SNP) analysis and in silico multi-locus sequence typing (MLST). Among the isolates sequenced, 5 phylogenetic clades were identified. The largest vanB clade belonged to MLST sequence type ST796 and contained clinical isolates from VREfm infections that clustered closely with isolates from colonised patients. Correlation of VREfm genotypes with spatio-temporal patient movements detected potential points of transmission within the hospital. ST80 emerged as the major vanA sequence type for which the most likely index case of a patient cluster was ascertained from SNP analyses. This work has identified the dominant clones associated with increased VREfm prevalence in a healthcare setting, and their likely direction of transmission. Nature Publishing Group UK 2018-04-19 /pmc/articles/PMC5908837/ /pubmed/29674657 http://dx.doi.org/10.1038/s41598-018-24614-6 Text en © The Author(s) 2018 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Leong, Kelvin W. C.
Cooley, Louise A.
Anderson, Tara L.
Gautam, Sanjay S.
McEwan, Belinda
Wells, Anne
Wilson, Fiona
Hughson, Lucy
O’Toole, Ronan F.
Emergence of Vancomycin-Resistant Enterococcus faecium at an Australian Hospital: A Whole Genome Sequencing Analysis
title Emergence of Vancomycin-Resistant Enterococcus faecium at an Australian Hospital: A Whole Genome Sequencing Analysis
title_full Emergence of Vancomycin-Resistant Enterococcus faecium at an Australian Hospital: A Whole Genome Sequencing Analysis
title_fullStr Emergence of Vancomycin-Resistant Enterococcus faecium at an Australian Hospital: A Whole Genome Sequencing Analysis
title_full_unstemmed Emergence of Vancomycin-Resistant Enterococcus faecium at an Australian Hospital: A Whole Genome Sequencing Analysis
title_short Emergence of Vancomycin-Resistant Enterococcus faecium at an Australian Hospital: A Whole Genome Sequencing Analysis
title_sort emergence of vancomycin-resistant enterococcus faecium at an australian hospital: a whole genome sequencing analysis
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5908837/
https://www.ncbi.nlm.nih.gov/pubmed/29674657
http://dx.doi.org/10.1038/s41598-018-24614-6
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