Cargando…

Near-infrared triggered strand displacement amplification for MicroRNA quantitative detection in single living cells

As an important modulator of gene expression, microRNA (miRNA) has been described as a promising biomarker for the early diagnosis of cancers. A non-invasive method for real-time sensitive imaging and monitoring of miRNA in living cells is in urgent demand. Although some amplified methods have been...

Descripción completa

Detalles Bibliográficos
Autores principales: Dai, Wenhao, Dong, Haifeng, Guo, Keke, Zhang, Xueji
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Royal Society of Chemistry 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5909124/
https://www.ncbi.nlm.nih.gov/pubmed/29732111
http://dx.doi.org/10.1039/c7sc04243d
_version_ 1783315837577330688
author Dai, Wenhao
Dong, Haifeng
Guo, Keke
Zhang, Xueji
author_facet Dai, Wenhao
Dong, Haifeng
Guo, Keke
Zhang, Xueji
author_sort Dai, Wenhao
collection PubMed
description As an important modulator of gene expression, microRNA (miRNA) has been described as a promising biomarker for the early diagnosis of cancers. A non-invasive method for real-time sensitive imaging and monitoring of miRNA in living cells is in urgent demand. Although some amplified methods have been developed, few can be programmed to assemble single intelligent nanostructures to realize sensitive intracellular miRNA detection without extra addition of an enzyme or catalytic fuel. Herein, two programmable oligonucleotide hairpin probe functionalized gold nanorods (THP-AuNRs) were designed to develop a near-infrared (NIR) laser triggered target strand displacement amplification (SDA) approach for sensitive miRNA imaging quantitative analysis in single living cells and multicellular tumor spheroids (MCTSs). Such a NIR-triggered SDA strategy achieves facile and sensitive monitoring of a model oncogenic miRNA-373 in various cancer lines and MCTS simulated tumor tissue. Notably, using a linear regression equation derived from miRNA mimics, a quantitative method of miRNA in single living cells was realized due to the high sensitivity. This provides a new way for sensitive real-time monitoring of intracellular miRNA, and may be promising for miRNA-based biomedical applications.
format Online
Article
Text
id pubmed-5909124
institution National Center for Biotechnology Information
language English
publishDate 2017
publisher Royal Society of Chemistry
record_format MEDLINE/PubMed
spelling pubmed-59091242018-05-04 Near-infrared triggered strand displacement amplification for MicroRNA quantitative detection in single living cells Dai, Wenhao Dong, Haifeng Guo, Keke Zhang, Xueji Chem Sci Chemistry As an important modulator of gene expression, microRNA (miRNA) has been described as a promising biomarker for the early diagnosis of cancers. A non-invasive method for real-time sensitive imaging and monitoring of miRNA in living cells is in urgent demand. Although some amplified methods have been developed, few can be programmed to assemble single intelligent nanostructures to realize sensitive intracellular miRNA detection without extra addition of an enzyme or catalytic fuel. Herein, two programmable oligonucleotide hairpin probe functionalized gold nanorods (THP-AuNRs) were designed to develop a near-infrared (NIR) laser triggered target strand displacement amplification (SDA) approach for sensitive miRNA imaging quantitative analysis in single living cells and multicellular tumor spheroids (MCTSs). Such a NIR-triggered SDA strategy achieves facile and sensitive monitoring of a model oncogenic miRNA-373 in various cancer lines and MCTS simulated tumor tissue. Notably, using a linear regression equation derived from miRNA mimics, a quantitative method of miRNA in single living cells was realized due to the high sensitivity. This provides a new way for sensitive real-time monitoring of intracellular miRNA, and may be promising for miRNA-based biomedical applications. Royal Society of Chemistry 2017-11-28 /pmc/articles/PMC5909124/ /pubmed/29732111 http://dx.doi.org/10.1039/c7sc04243d Text en This journal is © The Royal Society of Chemistry 2018 http://creativecommons.org/licenses/by-nc/3.0/ This article is freely available. This article is licensed under a Creative Commons Attribution Non Commercial 3.0 Unported Licence (CC BY-NC 3.0)
spellingShingle Chemistry
Dai, Wenhao
Dong, Haifeng
Guo, Keke
Zhang, Xueji
Near-infrared triggered strand displacement amplification for MicroRNA quantitative detection in single living cells
title Near-infrared triggered strand displacement amplification for MicroRNA quantitative detection in single living cells
title_full Near-infrared triggered strand displacement amplification for MicroRNA quantitative detection in single living cells
title_fullStr Near-infrared triggered strand displacement amplification for MicroRNA quantitative detection in single living cells
title_full_unstemmed Near-infrared triggered strand displacement amplification for MicroRNA quantitative detection in single living cells
title_short Near-infrared triggered strand displacement amplification for MicroRNA quantitative detection in single living cells
title_sort near-infrared triggered strand displacement amplification for microrna quantitative detection in single living cells
topic Chemistry
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5909124/
https://www.ncbi.nlm.nih.gov/pubmed/29732111
http://dx.doi.org/10.1039/c7sc04243d
work_keys_str_mv AT daiwenhao nearinfraredtriggeredstranddisplacementamplificationformicrornaquantitativedetectioninsinglelivingcells
AT donghaifeng nearinfraredtriggeredstranddisplacementamplificationformicrornaquantitativedetectioninsinglelivingcells
AT guokeke nearinfraredtriggeredstranddisplacementamplificationformicrornaquantitativedetectioninsinglelivingcells
AT zhangxueji nearinfraredtriggeredstranddisplacementamplificationformicrornaquantitativedetectioninsinglelivingcells