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Inducible and multiplex gene regulation using CRISPR-Cpf1-based transcription factors

Targeted and inducible regulation of mammalian gene expression is a broadly important capability. We engineered drug-inducible catalytically inactive Cpf1 fused to transcriptional activation domains to tune the expression of endogenous genes in human cells. Leveraging the multiplex capability of the...

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Detalles Bibliográficos
Autores principales:	Tak, Y. Esther, Kleinstiver, Benjamin P., Nuñez, James K., Hsu, Jonathan Y., Horng, Joy E., Gong, Jingyi, Weissman, Jonathan S., Joung, J. Keith
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	2017
Materias:	Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5909187/ https://www.ncbi.nlm.nih.gov/pubmed/29083402 http://dx.doi.org/10.1038/nmeth.4483

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