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Dielectrophoresis Testing of Nonlinear Viscoelastic Behaviors of Human Red Blood Cells

Dielectrophoresis in microfluidics provides a useful tool to test biomechanics of living cells, regardless of surface charges on cell membranes. We have designed an experimental method to characterize the nonlinear viscoelastic behaviors of single cells using dielectrophoresis in a microfluidic chan...

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Detalles Bibliográficos
Autores principales: Qiang, Yuhao, Liu, Jia, Du, E
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5909413/
https://www.ncbi.nlm.nih.gov/pubmed/29682335
http://dx.doi.org/10.3390/mi9010021
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author Qiang, Yuhao
Liu, Jia
Du, E
author_facet Qiang, Yuhao
Liu, Jia
Du, E
author_sort Qiang, Yuhao
collection PubMed
description Dielectrophoresis in microfluidics provides a useful tool to test biomechanics of living cells, regardless of surface charges on cell membranes. We have designed an experimental method to characterize the nonlinear viscoelastic behaviors of single cells using dielectrophoresis in a microfluidic channel. This method uses radio frequency, low voltage excitations through interdigitated microelectrodes, allowing probing multiple cells simultaneously with controllable load levels. Dielectrophoretic force was calibrated using a triaxial ellipsoid model. Using a Kelvin–Voigt model, the nonlinear shear moduli of cell membranes were determined from the steady-state deformations of red blood cells in response to a series of electric field strengths. The nonlinear elastic moduli of cell membranes ranged from 6.05 µN/m to up to 20.85 µN/m, which were identified as a function of extension ratio, rather than the lumped-parameter models as reported in the literature. Value of the characteristic time of the extensional recovery of cell membranes initially deformed to varied extent was found to be about 0.14 s. Shear viscosity of cell membrane was estimated to be 0.8–2.9 (µN/m)·s. This method is particularly valuable for rapid, non-invasive probing of mechanical properties of living cells.
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spelling pubmed-59094132018-04-20 Dielectrophoresis Testing of Nonlinear Viscoelastic Behaviors of Human Red Blood Cells Qiang, Yuhao Liu, Jia Du, E Micromachines (Basel) Article Dielectrophoresis in microfluidics provides a useful tool to test biomechanics of living cells, regardless of surface charges on cell membranes. We have designed an experimental method to characterize the nonlinear viscoelastic behaviors of single cells using dielectrophoresis in a microfluidic channel. This method uses radio frequency, low voltage excitations through interdigitated microelectrodes, allowing probing multiple cells simultaneously with controllable load levels. Dielectrophoretic force was calibrated using a triaxial ellipsoid model. Using a Kelvin–Voigt model, the nonlinear shear moduli of cell membranes were determined from the steady-state deformations of red blood cells in response to a series of electric field strengths. The nonlinear elastic moduli of cell membranes ranged from 6.05 µN/m to up to 20.85 µN/m, which were identified as a function of extension ratio, rather than the lumped-parameter models as reported in the literature. Value of the characteristic time of the extensional recovery of cell membranes initially deformed to varied extent was found to be about 0.14 s. Shear viscosity of cell membrane was estimated to be 0.8–2.9 (µN/m)·s. This method is particularly valuable for rapid, non-invasive probing of mechanical properties of living cells. MDPI 2018-01-09 /pmc/articles/PMC5909413/ /pubmed/29682335 http://dx.doi.org/10.3390/mi9010021 Text en © 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Qiang, Yuhao
Liu, Jia
Du, E
Dielectrophoresis Testing of Nonlinear Viscoelastic Behaviors of Human Red Blood Cells
title Dielectrophoresis Testing of Nonlinear Viscoelastic Behaviors of Human Red Blood Cells
title_full Dielectrophoresis Testing of Nonlinear Viscoelastic Behaviors of Human Red Blood Cells
title_fullStr Dielectrophoresis Testing of Nonlinear Viscoelastic Behaviors of Human Red Blood Cells
title_full_unstemmed Dielectrophoresis Testing of Nonlinear Viscoelastic Behaviors of Human Red Blood Cells
title_short Dielectrophoresis Testing of Nonlinear Viscoelastic Behaviors of Human Red Blood Cells
title_sort dielectrophoresis testing of nonlinear viscoelastic behaviors of human red blood cells
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5909413/
https://www.ncbi.nlm.nih.gov/pubmed/29682335
http://dx.doi.org/10.3390/mi9010021
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