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miR-30a suppresses osteosarcoma proliferation and metastasis by downregulating MEF2D expression

Many studies have revealed that microRNAs (miRNAs) play crucial roles in cancer development and progression. miRNA-30a (miR-30a), as a member of the miR-30 family, has been implicated in various cancers. However, the role of miR-30a in osteosarcoma remains unclear. In the current study, we found tha...

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Autores principales: Du, Liuxue, Chen, Tianpei, Zhao, Kai, Yang, Dong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Dove Medical Press 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5909778/
https://www.ncbi.nlm.nih.gov/pubmed/29713188
http://dx.doi.org/10.2147/OTT.S102430
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author Du, Liuxue
Chen, Tianpei
Zhao, Kai
Yang, Dong
author_facet Du, Liuxue
Chen, Tianpei
Zhao, Kai
Yang, Dong
author_sort Du, Liuxue
collection PubMed
description Many studies have revealed that microRNAs (miRNAs) play crucial roles in cancer development and progression. miRNA-30a (miR-30a), as a member of the miR-30 family, has been implicated in various cancers. However, the role of miR-30a in osteosarcoma remains unclear. In the current study, we found that miR-30a was significantly downregulated in osteosarcoma tissues and cell lines by using quantitative real-time polymerase chain reaction (qRT-PCR). In addition, miR-30a could inhibit cancer cell growth, migration, and invasion in vitro. Furthermore, bioinformatics of miRNA target prediction and luciferase reporter assay indicated that MEF2D is a direct target of miR-30a. miR-30a was able to reduce the mRNA and protein expression of MEF2D as assessed using RT-PCR and Western blotting assay. Interestingly, overexpression of MEF2D partially reversed the miR-30a-reduced cell proliferation, migration, and invasion of osteosarcoma cell, indicating that miR-30a suppresses osteosarcoma cell proliferation and metastasis partially mediated by inhibition of MEF2D. Overall, our study demonstrated that miR-30a functions as a tumor suppressor by targeting MEF2D in osteosarcoma, providing a promising prognostic biomarker and a therapeutic strategy for osteosarcoma.
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spelling pubmed-59097782018-04-30 miR-30a suppresses osteosarcoma proliferation and metastasis by downregulating MEF2D expression Du, Liuxue Chen, Tianpei Zhao, Kai Yang, Dong Onco Targets Ther Original Research Many studies have revealed that microRNAs (miRNAs) play crucial roles in cancer development and progression. miRNA-30a (miR-30a), as a member of the miR-30 family, has been implicated in various cancers. However, the role of miR-30a in osteosarcoma remains unclear. In the current study, we found that miR-30a was significantly downregulated in osteosarcoma tissues and cell lines by using quantitative real-time polymerase chain reaction (qRT-PCR). In addition, miR-30a could inhibit cancer cell growth, migration, and invasion in vitro. Furthermore, bioinformatics of miRNA target prediction and luciferase reporter assay indicated that MEF2D is a direct target of miR-30a. miR-30a was able to reduce the mRNA and protein expression of MEF2D as assessed using RT-PCR and Western blotting assay. Interestingly, overexpression of MEF2D partially reversed the miR-30a-reduced cell proliferation, migration, and invasion of osteosarcoma cell, indicating that miR-30a suppresses osteosarcoma cell proliferation and metastasis partially mediated by inhibition of MEF2D. Overall, our study demonstrated that miR-30a functions as a tumor suppressor by targeting MEF2D in osteosarcoma, providing a promising prognostic biomarker and a therapeutic strategy for osteosarcoma. Dove Medical Press 2018-04-17 /pmc/articles/PMC5909778/ /pubmed/29713188 http://dx.doi.org/10.2147/OTT.S102430 Text en © 2018 Du et al. This work is published and licensed by Dove Medical Press Limited The full terms of this license are available at https://www.dovepress.com/terms.php and incorporate the Creative Commons Attribution – Non Commercial (unported, v3.0) License (http://creativecommons.org/licenses/by-nc/3.0/). By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed.
spellingShingle Original Research
Du, Liuxue
Chen, Tianpei
Zhao, Kai
Yang, Dong
miR-30a suppresses osteosarcoma proliferation and metastasis by downregulating MEF2D expression
title miR-30a suppresses osteosarcoma proliferation and metastasis by downregulating MEF2D expression
title_full miR-30a suppresses osteosarcoma proliferation and metastasis by downregulating MEF2D expression
title_fullStr miR-30a suppresses osteosarcoma proliferation and metastasis by downregulating MEF2D expression
title_full_unstemmed miR-30a suppresses osteosarcoma proliferation and metastasis by downregulating MEF2D expression
title_short miR-30a suppresses osteosarcoma proliferation and metastasis by downregulating MEF2D expression
title_sort mir-30a suppresses osteosarcoma proliferation and metastasis by downregulating mef2d expression
topic Original Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5909778/
https://www.ncbi.nlm.nih.gov/pubmed/29713188
http://dx.doi.org/10.2147/OTT.S102430
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