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miR-30a suppresses osteosarcoma proliferation and metastasis by downregulating MEF2D expression
Many studies have revealed that microRNAs (miRNAs) play crucial roles in cancer development and progression. miRNA-30a (miR-30a), as a member of the miR-30 family, has been implicated in various cancers. However, the role of miR-30a in osteosarcoma remains unclear. In the current study, we found tha...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Dove Medical Press
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5909778/ https://www.ncbi.nlm.nih.gov/pubmed/29713188 http://dx.doi.org/10.2147/OTT.S102430 |
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author | Du, Liuxue Chen, Tianpei Zhao, Kai Yang, Dong |
author_facet | Du, Liuxue Chen, Tianpei Zhao, Kai Yang, Dong |
author_sort | Du, Liuxue |
collection | PubMed |
description | Many studies have revealed that microRNAs (miRNAs) play crucial roles in cancer development and progression. miRNA-30a (miR-30a), as a member of the miR-30 family, has been implicated in various cancers. However, the role of miR-30a in osteosarcoma remains unclear. In the current study, we found that miR-30a was significantly downregulated in osteosarcoma tissues and cell lines by using quantitative real-time polymerase chain reaction (qRT-PCR). In addition, miR-30a could inhibit cancer cell growth, migration, and invasion in vitro. Furthermore, bioinformatics of miRNA target prediction and luciferase reporter assay indicated that MEF2D is a direct target of miR-30a. miR-30a was able to reduce the mRNA and protein expression of MEF2D as assessed using RT-PCR and Western blotting assay. Interestingly, overexpression of MEF2D partially reversed the miR-30a-reduced cell proliferation, migration, and invasion of osteosarcoma cell, indicating that miR-30a suppresses osteosarcoma cell proliferation and metastasis partially mediated by inhibition of MEF2D. Overall, our study demonstrated that miR-30a functions as a tumor suppressor by targeting MEF2D in osteosarcoma, providing a promising prognostic biomarker and a therapeutic strategy for osteosarcoma. |
format | Online Article Text |
id | pubmed-5909778 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Dove Medical Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-59097782018-04-30 miR-30a suppresses osteosarcoma proliferation and metastasis by downregulating MEF2D expression Du, Liuxue Chen, Tianpei Zhao, Kai Yang, Dong Onco Targets Ther Original Research Many studies have revealed that microRNAs (miRNAs) play crucial roles in cancer development and progression. miRNA-30a (miR-30a), as a member of the miR-30 family, has been implicated in various cancers. However, the role of miR-30a in osteosarcoma remains unclear. In the current study, we found that miR-30a was significantly downregulated in osteosarcoma tissues and cell lines by using quantitative real-time polymerase chain reaction (qRT-PCR). In addition, miR-30a could inhibit cancer cell growth, migration, and invasion in vitro. Furthermore, bioinformatics of miRNA target prediction and luciferase reporter assay indicated that MEF2D is a direct target of miR-30a. miR-30a was able to reduce the mRNA and protein expression of MEF2D as assessed using RT-PCR and Western blotting assay. Interestingly, overexpression of MEF2D partially reversed the miR-30a-reduced cell proliferation, migration, and invasion of osteosarcoma cell, indicating that miR-30a suppresses osteosarcoma cell proliferation and metastasis partially mediated by inhibition of MEF2D. Overall, our study demonstrated that miR-30a functions as a tumor suppressor by targeting MEF2D in osteosarcoma, providing a promising prognostic biomarker and a therapeutic strategy for osteosarcoma. Dove Medical Press 2018-04-17 /pmc/articles/PMC5909778/ /pubmed/29713188 http://dx.doi.org/10.2147/OTT.S102430 Text en © 2018 Du et al. This work is published and licensed by Dove Medical Press Limited The full terms of this license are available at https://www.dovepress.com/terms.php and incorporate the Creative Commons Attribution – Non Commercial (unported, v3.0) License (http://creativecommons.org/licenses/by-nc/3.0/). By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed. |
spellingShingle | Original Research Du, Liuxue Chen, Tianpei Zhao, Kai Yang, Dong miR-30a suppresses osteosarcoma proliferation and metastasis by downregulating MEF2D expression |
title | miR-30a suppresses osteosarcoma proliferation and metastasis by downregulating MEF2D expression |
title_full | miR-30a suppresses osteosarcoma proliferation and metastasis by downregulating MEF2D expression |
title_fullStr | miR-30a suppresses osteosarcoma proliferation and metastasis by downregulating MEF2D expression |
title_full_unstemmed | miR-30a suppresses osteosarcoma proliferation and metastasis by downregulating MEF2D expression |
title_short | miR-30a suppresses osteosarcoma proliferation and metastasis by downregulating MEF2D expression |
title_sort | mir-30a suppresses osteosarcoma proliferation and metastasis by downregulating mef2d expression |
topic | Original Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5909778/ https://www.ncbi.nlm.nih.gov/pubmed/29713188 http://dx.doi.org/10.2147/OTT.S102430 |
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