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Targeted Drug Delivery in the Suprachoroidal Space by Swollen Hydrogel Pushing
PURPOSE: The purpose is to target model drug particles to the posterior region of the suprachoroidal space (SCS) of the eye controlled via pushing by hydrogel swelling. METHODS: A particle formulation containing 1% hyaluronic acid (HA) with fluorescent polymer particles and a hydrogel formulation co...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
The Association for Research in Vision and Ophthalmology
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5909801/ https://www.ncbi.nlm.nih.gov/pubmed/29677369 http://dx.doi.org/10.1167/iovs.17-23758 |
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author | Jung, Jae Hwan Desit, Patcharin Prausnitz, Mark R. |
author_facet | Jung, Jae Hwan Desit, Patcharin Prausnitz, Mark R. |
author_sort | Jung, Jae Hwan |
collection | PubMed |
description | PURPOSE: The purpose is to target model drug particles to the posterior region of the suprachoroidal space (SCS) of the eye controlled via pushing by hydrogel swelling. METHODS: A particle formulation containing 1% hyaluronic acid (HA) with fluorescent polymer particles and a hydrogel formulation containing 4% HA were introduced in a single syringe as two layers without mixing, and injected sequentially into the SCS of the rabbit eye ex vivo and in vivo using a microneedle. Distribution of particles in the eye was determined by microscopy. RESULTS: During injection, the particle formulation was pushed toward the middle of the SCS by the viscous hydrogel formulation, but less than 12% of particles reached the posterior SCS. After injection, the particle formulation was pushed further toward the macula and optic nerve in the posterior SCS by hydrogel swelling and spreading. Heating the eye to 37°C, or injecting in vivo decreased viscosity and mechanical strength of the hydrogel, thereby allowing it to swell and flow further in the SCS. A high salt concentration (9% NaCl) in the hydrogel formulation further increased hydrogel swelling due to osmotic flow into the hydrogel. In this way, up to 76% of particles were delivered to the posterior SCS from an injection made near the limbus. CONCLUSIONS: This study shows that model drug particles can be targeted to the posterior SCS by HA hydrogel swelling and pushing without particle functionalization or administering external driving forces. |
format | Online Article Text |
id | pubmed-5909801 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | The Association for Research in Vision and Ophthalmology |
record_format | MEDLINE/PubMed |
spelling | pubmed-59098012018-04-22 Targeted Drug Delivery in the Suprachoroidal Space by Swollen Hydrogel Pushing Jung, Jae Hwan Desit, Patcharin Prausnitz, Mark R. Invest Ophthalmol Vis Sci Retina PURPOSE: The purpose is to target model drug particles to the posterior region of the suprachoroidal space (SCS) of the eye controlled via pushing by hydrogel swelling. METHODS: A particle formulation containing 1% hyaluronic acid (HA) with fluorescent polymer particles and a hydrogel formulation containing 4% HA were introduced in a single syringe as two layers without mixing, and injected sequentially into the SCS of the rabbit eye ex vivo and in vivo using a microneedle. Distribution of particles in the eye was determined by microscopy. RESULTS: During injection, the particle formulation was pushed toward the middle of the SCS by the viscous hydrogel formulation, but less than 12% of particles reached the posterior SCS. After injection, the particle formulation was pushed further toward the macula and optic nerve in the posterior SCS by hydrogel swelling and spreading. Heating the eye to 37°C, or injecting in vivo decreased viscosity and mechanical strength of the hydrogel, thereby allowing it to swell and flow further in the SCS. A high salt concentration (9% NaCl) in the hydrogel formulation further increased hydrogel swelling due to osmotic flow into the hydrogel. In this way, up to 76% of particles were delivered to the posterior SCS from an injection made near the limbus. CONCLUSIONS: This study shows that model drug particles can be targeted to the posterior SCS by HA hydrogel swelling and pushing without particle functionalization or administering external driving forces. The Association for Research in Vision and Ophthalmology 2018-04 /pmc/articles/PMC5909801/ /pubmed/29677369 http://dx.doi.org/10.1167/iovs.17-23758 Text en Copyright 2018 The Authors http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. |
spellingShingle | Retina Jung, Jae Hwan Desit, Patcharin Prausnitz, Mark R. Targeted Drug Delivery in the Suprachoroidal Space by Swollen Hydrogel Pushing |
title | Targeted Drug Delivery in the Suprachoroidal Space by Swollen Hydrogel Pushing |
title_full | Targeted Drug Delivery in the Suprachoroidal Space by Swollen Hydrogel Pushing |
title_fullStr | Targeted Drug Delivery in the Suprachoroidal Space by Swollen Hydrogel Pushing |
title_full_unstemmed | Targeted Drug Delivery in the Suprachoroidal Space by Swollen Hydrogel Pushing |
title_short | Targeted Drug Delivery in the Suprachoroidal Space by Swollen Hydrogel Pushing |
title_sort | targeted drug delivery in the suprachoroidal space by swollen hydrogel pushing |
topic | Retina |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5909801/ https://www.ncbi.nlm.nih.gov/pubmed/29677369 http://dx.doi.org/10.1167/iovs.17-23758 |
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