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Profiling of 179 miRNA Expression in Blood Plasma of Lung Cancer Patients and Cancer-Free Individuals
Lung cancer is one of major cancers, and survival of lung cancer patients is dictated by the timely detection and diagnosis. Cell-free circulating miRNAs were proposed as candidate biomarkers for lung cancer. These RNAs are frequently deregulated in lung cancer and can persist in bodily fluids for e...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5910392/ https://www.ncbi.nlm.nih.gov/pubmed/29679068 http://dx.doi.org/10.1038/s41598-018-24769-2 |
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author | Zaporozhchenko, Ivan A. Morozkin, Evgeny S. Ponomaryova, Anastasia A. Rykova, Elena Y. Cherdyntseva, Nadezhda V. Zheravin, Aleksandr A. Pashkovskaya, Oksana A. Pokushalov, Evgeny A. Vlassov, Valentin V. Laktionov, Pavel P. |
author_facet | Zaporozhchenko, Ivan A. Morozkin, Evgeny S. Ponomaryova, Anastasia A. Rykova, Elena Y. Cherdyntseva, Nadezhda V. Zheravin, Aleksandr A. Pashkovskaya, Oksana A. Pokushalov, Evgeny A. Vlassov, Valentin V. Laktionov, Pavel P. |
author_sort | Zaporozhchenko, Ivan A. |
collection | PubMed |
description | Lung cancer is one of major cancers, and survival of lung cancer patients is dictated by the timely detection and diagnosis. Cell-free circulating miRNAs were proposed as candidate biomarkers for lung cancer. These RNAs are frequently deregulated in lung cancer and can persist in bodily fluids for extended periods of time, shielded from degradation by membrane vesicles and biopolymer complexes. To date, several groups reported the presence of lung tumour-specific subsets of miRNAs in blood. Here we describe the profiling of blood plasma miRNAs in lung cancer patients, healthy individuals and endobronchitis patients using miRCURY LNA miRNA qPCR Serum/Plasma Panel (Exiqon). From 241 ratios differently expressed between cancer patients and healthy individuals 19 miRNAs were selected for verification using the same platform. LASSO-penalized logistic regression model, including 10 miRNA ratios comprised of 14 individual miRNAs discriminated lung cancer patients from both control groups with AUC of 0.979. |
format | Online Article Text |
id | pubmed-5910392 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-59103922018-04-30 Profiling of 179 miRNA Expression in Blood Plasma of Lung Cancer Patients and Cancer-Free Individuals Zaporozhchenko, Ivan A. Morozkin, Evgeny S. Ponomaryova, Anastasia A. Rykova, Elena Y. Cherdyntseva, Nadezhda V. Zheravin, Aleksandr A. Pashkovskaya, Oksana A. Pokushalov, Evgeny A. Vlassov, Valentin V. Laktionov, Pavel P. Sci Rep Article Lung cancer is one of major cancers, and survival of lung cancer patients is dictated by the timely detection and diagnosis. Cell-free circulating miRNAs were proposed as candidate biomarkers for lung cancer. These RNAs are frequently deregulated in lung cancer and can persist in bodily fluids for extended periods of time, shielded from degradation by membrane vesicles and biopolymer complexes. To date, several groups reported the presence of lung tumour-specific subsets of miRNAs in blood. Here we describe the profiling of blood plasma miRNAs in lung cancer patients, healthy individuals and endobronchitis patients using miRCURY LNA miRNA qPCR Serum/Plasma Panel (Exiqon). From 241 ratios differently expressed between cancer patients and healthy individuals 19 miRNAs were selected for verification using the same platform. LASSO-penalized logistic regression model, including 10 miRNA ratios comprised of 14 individual miRNAs discriminated lung cancer patients from both control groups with AUC of 0.979. Nature Publishing Group UK 2018-04-20 /pmc/articles/PMC5910392/ /pubmed/29679068 http://dx.doi.org/10.1038/s41598-018-24769-2 Text en © The Author(s) 2018 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Article Zaporozhchenko, Ivan A. Morozkin, Evgeny S. Ponomaryova, Anastasia A. Rykova, Elena Y. Cherdyntseva, Nadezhda V. Zheravin, Aleksandr A. Pashkovskaya, Oksana A. Pokushalov, Evgeny A. Vlassov, Valentin V. Laktionov, Pavel P. Profiling of 179 miRNA Expression in Blood Plasma of Lung Cancer Patients and Cancer-Free Individuals |
title | Profiling of 179 miRNA Expression in Blood Plasma of Lung Cancer Patients and Cancer-Free Individuals |
title_full | Profiling of 179 miRNA Expression in Blood Plasma of Lung Cancer Patients and Cancer-Free Individuals |
title_fullStr | Profiling of 179 miRNA Expression in Blood Plasma of Lung Cancer Patients and Cancer-Free Individuals |
title_full_unstemmed | Profiling of 179 miRNA Expression in Blood Plasma of Lung Cancer Patients and Cancer-Free Individuals |
title_short | Profiling of 179 miRNA Expression in Blood Plasma of Lung Cancer Patients and Cancer-Free Individuals |
title_sort | profiling of 179 mirna expression in blood plasma of lung cancer patients and cancer-free individuals |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5910392/ https://www.ncbi.nlm.nih.gov/pubmed/29679068 http://dx.doi.org/10.1038/s41598-018-24769-2 |
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