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Development of Harmaline-induced Tremor in a Swine Model

BACKGROUND: In the field of translational neuroscience research, it is critical to utilize a large animal model to test the feasibility, safety, and functionality of novel therapies. Here, we describe a protocol for the development of a large animal model of tremor. METHODS: In a pig model, tremor w...

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Autores principales: Lee, Jihyun, Kim, Inyong, Lee, Jeyeon, Knight, Emily, Cheng, Lei, Kang, Shin il, Jang, Dong Pyo, Chang, Su-Youne
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Columbia University Libraries/Information Services 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5910538/
https://www.ncbi.nlm.nih.gov/pubmed/29686939
http://dx.doi.org/10.7916/D8J68TV7
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author Lee, Jihyun
Kim, Inyong
Lee, Jeyeon
Knight, Emily
Cheng, Lei
Kang, Shin il
Jang, Dong Pyo
Chang, Su-Youne
author_facet Lee, Jihyun
Kim, Inyong
Lee, Jeyeon
Knight, Emily
Cheng, Lei
Kang, Shin il
Jang, Dong Pyo
Chang, Su-Youne
author_sort Lee, Jihyun
collection PubMed
description BACKGROUND: In the field of translational neuroscience research, it is critical to utilize a large animal model to test the feasibility, safety, and functionality of novel therapies. Here, we describe a protocol for the development of a large animal model of tremor. METHODS: In a pig model, tremor was induced with harmaline and measured with wireless accelerometers attached to the limbs. Three different doses of harmaline were tested and three repetitive injections were made at 72-hour intervals. To fully characterize the drug-induced tremor, onset time, tremor amplitude, maintained duration, and peak tremor frequency were analyzed. RESULTS: Harmaline-induced tremor appeared immediately following intravenous injection of harmaline. Tremor was maintained over 2 hours. Its frequency was 10–16 Hz, which was independent of doses. Dose-dependent responses were observed in tremor amplitude, triggering time, and tremor-maintained duration. Repetitive injection of harmaline desensitized the harmaline effect. DISCUSSION: We provide a detailed protocol for training, drug injection, device selection, and tremor recording optimized to create a swine model of tremor with harmaline. Our protocol provides reliable tremor in pigs and suggests pig as a valid translational large animal model of tremor.
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spelling pubmed-59105382018-04-23 Development of Harmaline-induced Tremor in a Swine Model Lee, Jihyun Kim, Inyong Lee, Jeyeon Knight, Emily Cheng, Lei Kang, Shin il Jang, Dong Pyo Chang, Su-Youne Tremor Other Hyperkinet Mov (N Y) Brief Reports BACKGROUND: In the field of translational neuroscience research, it is critical to utilize a large animal model to test the feasibility, safety, and functionality of novel therapies. Here, we describe a protocol for the development of a large animal model of tremor. METHODS: In a pig model, tremor was induced with harmaline and measured with wireless accelerometers attached to the limbs. Three different doses of harmaline were tested and three repetitive injections were made at 72-hour intervals. To fully characterize the drug-induced tremor, onset time, tremor amplitude, maintained duration, and peak tremor frequency were analyzed. RESULTS: Harmaline-induced tremor appeared immediately following intravenous injection of harmaline. Tremor was maintained over 2 hours. Its frequency was 10–16 Hz, which was independent of doses. Dose-dependent responses were observed in tremor amplitude, triggering time, and tremor-maintained duration. Repetitive injection of harmaline desensitized the harmaline effect. DISCUSSION: We provide a detailed protocol for training, drug injection, device selection, and tremor recording optimized to create a swine model of tremor with harmaline. Our protocol provides reliable tremor in pigs and suggests pig as a valid translational large animal model of tremor. Columbia University Libraries/Information Services 2018-03-13 /pmc/articles/PMC5910538/ /pubmed/29686939 http://dx.doi.org/10.7916/D8J68TV7 Text en © 2018 Lee et al. http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution–Noncommerical–No Derivatives License, which permits the user to copy, distribute, and transmit the work provided that the original author and source are credited; that no commercial use is made of the work; and that the work is not altered or transformed.
spellingShingle Brief Reports
Lee, Jihyun
Kim, Inyong
Lee, Jeyeon
Knight, Emily
Cheng, Lei
Kang, Shin il
Jang, Dong Pyo
Chang, Su-Youne
Development of Harmaline-induced Tremor in a Swine Model
title Development of Harmaline-induced Tremor in a Swine Model
title_full Development of Harmaline-induced Tremor in a Swine Model
title_fullStr Development of Harmaline-induced Tremor in a Swine Model
title_full_unstemmed Development of Harmaline-induced Tremor in a Swine Model
title_short Development of Harmaline-induced Tremor in a Swine Model
title_sort development of harmaline-induced tremor in a swine model
topic Brief Reports
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5910538/
https://www.ncbi.nlm.nih.gov/pubmed/29686939
http://dx.doi.org/10.7916/D8J68TV7
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