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Laccase from Aspergillus niger: A novel tool to graft multifunctional materials of interests and their characterization

In the present study, we propose a green route to prepare poly(3-hydroxybutyrate) [(P(3HB)] grafted ethyl cellulose (EC) based green composites with novel characteristics through laccase-assisted grafting. P(3HB) was used as a side chain whereas, EC as a backbone material under ambient processing co...

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Autores principales: Iqbal, Hafiz M.N., Kyazze, Godfrey, Tron, Thierry, Keshavarz, Tajalli
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5910650/
https://www.ncbi.nlm.nih.gov/pubmed/29686517
http://dx.doi.org/10.1016/j.sjbs.2016.01.027
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author Iqbal, Hafiz M.N.
Kyazze, Godfrey
Tron, Thierry
Keshavarz, Tajalli
author_facet Iqbal, Hafiz M.N.
Kyazze, Godfrey
Tron, Thierry
Keshavarz, Tajalli
author_sort Iqbal, Hafiz M.N.
collection PubMed
description In the present study, we propose a green route to prepare poly(3-hydroxybutyrate) [(P(3HB)] grafted ethyl cellulose (EC) based green composites with novel characteristics through laccase-assisted grafting. P(3HB) was used as a side chain whereas, EC as a backbone material under ambient processing conditions. A novel laccase obtained from Aspergillus niger through its heterologous expression in Saccharomyces cerevisiae was used as a green catalyst for grafting purposes without the use of additional initiator and/or cross-linking agents. Subsequently, the resulting P(3HB)-g-EC composites were characterized using a range of analytical and imagining techniques. Fourier transform infrared spectroscopy (FT-IR) spectra showed an increase in the hydrogen-bonding type interactions between the side chains of P(3HB) and backbone material of EC. Evidently, X-ray diffraction (XRD) analysis revealed a decrease in the crystallinity of the P(3HB)-g-EC composites as compared to the pristine individual polymers. A homogeneous P(3HB) distribution was also achieved in case of the graft composite prepared in the presence of 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS) as a mediator along with laccase as compared to the composite prepared using pure laccase alone. A substantial improvement in the thermal and mechanical characteristics was observed for grafted composites up to the different extent as compared to the pristine counterparts. The hydrophobic/hydrophilic properties of the grafted composites were better than those of the pristine counterparts.
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spelling pubmed-59106502018-04-23 Laccase from Aspergillus niger: A novel tool to graft multifunctional materials of interests and their characterization Iqbal, Hafiz M.N. Kyazze, Godfrey Tron, Thierry Keshavarz, Tajalli Saudi J Biol Sci Original Article In the present study, we propose a green route to prepare poly(3-hydroxybutyrate) [(P(3HB)] grafted ethyl cellulose (EC) based green composites with novel characteristics through laccase-assisted grafting. P(3HB) was used as a side chain whereas, EC as a backbone material under ambient processing conditions. A novel laccase obtained from Aspergillus niger through its heterologous expression in Saccharomyces cerevisiae was used as a green catalyst for grafting purposes without the use of additional initiator and/or cross-linking agents. Subsequently, the resulting P(3HB)-g-EC composites were characterized using a range of analytical and imagining techniques. Fourier transform infrared spectroscopy (FT-IR) spectra showed an increase in the hydrogen-bonding type interactions between the side chains of P(3HB) and backbone material of EC. Evidently, X-ray diffraction (XRD) analysis revealed a decrease in the crystallinity of the P(3HB)-g-EC composites as compared to the pristine individual polymers. A homogeneous P(3HB) distribution was also achieved in case of the graft composite prepared in the presence of 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS) as a mediator along with laccase as compared to the composite prepared using pure laccase alone. A substantial improvement in the thermal and mechanical characteristics was observed for grafted composites up to the different extent as compared to the pristine counterparts. The hydrophobic/hydrophilic properties of the grafted composites were better than those of the pristine counterparts. Elsevier 2018-03 2016-01-21 /pmc/articles/PMC5910650/ /pubmed/29686517 http://dx.doi.org/10.1016/j.sjbs.2016.01.027 Text en © 2016 The Authors https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Original Article
Iqbal, Hafiz M.N.
Kyazze, Godfrey
Tron, Thierry
Keshavarz, Tajalli
Laccase from Aspergillus niger: A novel tool to graft multifunctional materials of interests and their characterization
title Laccase from Aspergillus niger: A novel tool to graft multifunctional materials of interests and their characterization
title_full Laccase from Aspergillus niger: A novel tool to graft multifunctional materials of interests and their characterization
title_fullStr Laccase from Aspergillus niger: A novel tool to graft multifunctional materials of interests and their characterization
title_full_unstemmed Laccase from Aspergillus niger: A novel tool to graft multifunctional materials of interests and their characterization
title_short Laccase from Aspergillus niger: A novel tool to graft multifunctional materials of interests and their characterization
title_sort laccase from aspergillus niger: a novel tool to graft multifunctional materials of interests and their characterization
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5910650/
https://www.ncbi.nlm.nih.gov/pubmed/29686517
http://dx.doi.org/10.1016/j.sjbs.2016.01.027
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