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Flavonoid biosynthesis controls fiber color in naturally colored cotton
The existence of only natural brown and green cotton fibers (BCF and GCF, respectively), as well as poor fiber quality, limits the use of naturally colored cotton (Gossypium hirsutum L.). A better understanding of fiber pigment regulation is needed to surmount these obstacles. In this work, transcri...
Autores principales: | , , , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
PeerJ Inc.
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5910794/ https://www.ncbi.nlm.nih.gov/pubmed/29682406 http://dx.doi.org/10.7717/peerj.4537 |
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author | Liu, Hai-Feng Luo, Cheng Song, Wu Shen, Haitao Li, Guoliang He, Zhi-Gang Chen, Wen-Gang Cao, Yan-Yan Huang, Fang Tang, Shou-Wu Hong, Ping Zhao, En-Feng Zhu, Jianbo He, Dajun Wang, Shaoming Huo, Guang-Ying Liu, Hailiang |
author_facet | Liu, Hai-Feng Luo, Cheng Song, Wu Shen, Haitao Li, Guoliang He, Zhi-Gang Chen, Wen-Gang Cao, Yan-Yan Huang, Fang Tang, Shou-Wu Hong, Ping Zhao, En-Feng Zhu, Jianbo He, Dajun Wang, Shaoming Huo, Guang-Ying Liu, Hailiang |
author_sort | Liu, Hai-Feng |
collection | PubMed |
description | The existence of only natural brown and green cotton fibers (BCF and GCF, respectively), as well as poor fiber quality, limits the use of naturally colored cotton (Gossypium hirsutum L.). A better understanding of fiber pigment regulation is needed to surmount these obstacles. In this work, transcriptome analysis and quantitative reverse transcription PCR revealed that 13 and 9 phenylpropanoid (metabolic) pathway genes were enriched during pigment synthesis, while the differential expression of phenylpropanoid (metabolic) and flavonoid metabolic pathway genes occurred among BCF, GCF, and white cotton fibers (WCF). Silencing the chalcone flavanone isomerase gene in a BCF line resulted in three fiber phenotypes among offspring of the RNAi lines: BCF, almost WCF, and GCF. The lines with almost WCF suppressed chalcone flavanone isomerase, while the lines with GCF highly expressed the glucosyl transferase (3GT) gene. Overexpression of the Gh3GT or Arabidopsis thaliana 3GT gene in BCF lines resulted in GCF. Additionally, the phenylpropanoid and flavonoid metabolites of BCF and GCF were significantly higher than those of WCF as assessed by a metabolomics analysis. Thus, the flavonoid biosynthetic pathway controls both brown and green pigmentation processes. Like natural colored fibers, the transgenic colored fibers were weaker and shorter than WCF. This study shows the potential of flavonoid pathway modifications to alter cotton fibers’ color and quality. |
format | Online Article Text |
id | pubmed-5910794 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | PeerJ Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-59107942018-04-22 Flavonoid biosynthesis controls fiber color in naturally colored cotton Liu, Hai-Feng Luo, Cheng Song, Wu Shen, Haitao Li, Guoliang He, Zhi-Gang Chen, Wen-Gang Cao, Yan-Yan Huang, Fang Tang, Shou-Wu Hong, Ping Zhao, En-Feng Zhu, Jianbo He, Dajun Wang, Shaoming Huo, Guang-Ying Liu, Hailiang PeerJ Bioinformatics The existence of only natural brown and green cotton fibers (BCF and GCF, respectively), as well as poor fiber quality, limits the use of naturally colored cotton (Gossypium hirsutum L.). A better understanding of fiber pigment regulation is needed to surmount these obstacles. In this work, transcriptome analysis and quantitative reverse transcription PCR revealed that 13 and 9 phenylpropanoid (metabolic) pathway genes were enriched during pigment synthesis, while the differential expression of phenylpropanoid (metabolic) and flavonoid metabolic pathway genes occurred among BCF, GCF, and white cotton fibers (WCF). Silencing the chalcone flavanone isomerase gene in a BCF line resulted in three fiber phenotypes among offspring of the RNAi lines: BCF, almost WCF, and GCF. The lines with almost WCF suppressed chalcone flavanone isomerase, while the lines with GCF highly expressed the glucosyl transferase (3GT) gene. Overexpression of the Gh3GT or Arabidopsis thaliana 3GT gene in BCF lines resulted in GCF. Additionally, the phenylpropanoid and flavonoid metabolites of BCF and GCF were significantly higher than those of WCF as assessed by a metabolomics analysis. Thus, the flavonoid biosynthetic pathway controls both brown and green pigmentation processes. Like natural colored fibers, the transgenic colored fibers were weaker and shorter than WCF. This study shows the potential of flavonoid pathway modifications to alter cotton fibers’ color and quality. PeerJ Inc. 2018-04-18 /pmc/articles/PMC5910794/ /pubmed/29682406 http://dx.doi.org/10.7717/peerj.4537 Text en ©2018 Liu et al. http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, reproduction and adaptation in any medium and for any purpose provided that it is properly attributed. For attribution, the original author(s), title, publication source (PeerJ) and either DOI or URL of the article must be cited. |
spellingShingle | Bioinformatics Liu, Hai-Feng Luo, Cheng Song, Wu Shen, Haitao Li, Guoliang He, Zhi-Gang Chen, Wen-Gang Cao, Yan-Yan Huang, Fang Tang, Shou-Wu Hong, Ping Zhao, En-Feng Zhu, Jianbo He, Dajun Wang, Shaoming Huo, Guang-Ying Liu, Hailiang Flavonoid biosynthesis controls fiber color in naturally colored cotton |
title | Flavonoid biosynthesis controls fiber color in naturally colored cotton |
title_full | Flavonoid biosynthesis controls fiber color in naturally colored cotton |
title_fullStr | Flavonoid biosynthesis controls fiber color in naturally colored cotton |
title_full_unstemmed | Flavonoid biosynthesis controls fiber color in naturally colored cotton |
title_short | Flavonoid biosynthesis controls fiber color in naturally colored cotton |
title_sort | flavonoid biosynthesis controls fiber color in naturally colored cotton |
topic | Bioinformatics |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5910794/ https://www.ncbi.nlm.nih.gov/pubmed/29682406 http://dx.doi.org/10.7717/peerj.4537 |
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