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Response of macrophages in rat skeletal muscle after eccentric exercise
PURPOSE: Macrophages are known to be important for healing numerous injured tissues depending on their functional phenotypes in response to different stimuli. The objective of this study was to reveal macrophage phenotypic changes involved in exercise-induced skeletal muscle injury and regeneration....
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5911737/ https://www.ncbi.nlm.nih.gov/pubmed/29550244 http://dx.doi.org/10.1016/j.cjtee.2017.12.001 |
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author | Zuo, Qun Wang, Shu-Chen Yu, Xin-Kai Chao, Wei-Wei |
author_facet | Zuo, Qun Wang, Shu-Chen Yu, Xin-Kai Chao, Wei-Wei |
author_sort | Zuo, Qun |
collection | PubMed |
description | PURPOSE: Macrophages are known to be important for healing numerous injured tissues depending on their functional phenotypes in response to different stimuli. The objective of this study was to reveal macrophage phenotypic changes involved in exercise-induced skeletal muscle injury and regeneration. METHODS: Adult male Sprague-Dawley rats experienced one session of downhill running (16° decline, 16 m/min) for 90 min. After exercise the blood and soleus muscles were collected at 0 h, 6 h, 12 h, 1 d, 2 d, 3 d, 1 w and 2 w after exercise, separately. RESULTS: It was showed that CD68(+) M1 macrophages mainly infiltrated into muscle necrotic sites at 1–3 d, while CD163(+) M2 macrophages were present in muscles from 0 h to 2 weeks after exercise. Using transmission electron microscopy, we observed activated satellite cells 1 d after exercise. Th1-associated transcripts of iNOS and Ccl2 were inhibited post exercise, while COX-2 mRNA was dramatically increased 12 h after running (p < 0.01). M2 phenotype marker Arg-1 increased 12 h and 3 d (p < 0.05, p < 0.01) after exercise, and Clec10a and Mrc2 were up-regulated in muscles 12 h following exercise (p < 0.05, p < 0.05). CONCLUSION: The data demonstrate the dynamic patterns of macrophage phenotype in skeletal muscle upon eccentric exercise stimuli, and M1 and M2 phenotypes perform different functions during exercise-induced skeletal muscle injury and recovery. |
format | Online Article Text |
id | pubmed-5911737 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Elsevier |
record_format | MEDLINE/PubMed |
spelling | pubmed-59117372018-04-24 Response of macrophages in rat skeletal muscle after eccentric exercise Zuo, Qun Wang, Shu-Chen Yu, Xin-Kai Chao, Wei-Wei Chin J Traumatol Original Article PURPOSE: Macrophages are known to be important for healing numerous injured tissues depending on their functional phenotypes in response to different stimuli. The objective of this study was to reveal macrophage phenotypic changes involved in exercise-induced skeletal muscle injury and regeneration. METHODS: Adult male Sprague-Dawley rats experienced one session of downhill running (16° decline, 16 m/min) for 90 min. After exercise the blood and soleus muscles were collected at 0 h, 6 h, 12 h, 1 d, 2 d, 3 d, 1 w and 2 w after exercise, separately. RESULTS: It was showed that CD68(+) M1 macrophages mainly infiltrated into muscle necrotic sites at 1–3 d, while CD163(+) M2 macrophages were present in muscles from 0 h to 2 weeks after exercise. Using transmission electron microscopy, we observed activated satellite cells 1 d after exercise. Th1-associated transcripts of iNOS and Ccl2 were inhibited post exercise, while COX-2 mRNA was dramatically increased 12 h after running (p < 0.01). M2 phenotype marker Arg-1 increased 12 h and 3 d (p < 0.05, p < 0.01) after exercise, and Clec10a and Mrc2 were up-regulated in muscles 12 h following exercise (p < 0.05, p < 0.05). CONCLUSION: The data demonstrate the dynamic patterns of macrophage phenotype in skeletal muscle upon eccentric exercise stimuli, and M1 and M2 phenotypes perform different functions during exercise-induced skeletal muscle injury and recovery. Elsevier 2018-04 2018-02-20 /pmc/articles/PMC5911737/ /pubmed/29550244 http://dx.doi.org/10.1016/j.cjtee.2017.12.001 Text en © 2018 Daping Hospital and the Research Institute of Surgery of the Third Military Medical University. Production and hosting by Elsevier B.V. http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). |
spellingShingle | Original Article Zuo, Qun Wang, Shu-Chen Yu, Xin-Kai Chao, Wei-Wei Response of macrophages in rat skeletal muscle after eccentric exercise |
title | Response of macrophages in rat skeletal muscle after eccentric exercise |
title_full | Response of macrophages in rat skeletal muscle after eccentric exercise |
title_fullStr | Response of macrophages in rat skeletal muscle after eccentric exercise |
title_full_unstemmed | Response of macrophages in rat skeletal muscle after eccentric exercise |
title_short | Response of macrophages in rat skeletal muscle after eccentric exercise |
title_sort | response of macrophages in rat skeletal muscle after eccentric exercise |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5911737/ https://www.ncbi.nlm.nih.gov/pubmed/29550244 http://dx.doi.org/10.1016/j.cjtee.2017.12.001 |
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