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High‐throughput analysis of anammox bacteria in wetland and dryland soils along the altitudinal gradient in Qinghai–Tibet Plateau

This study investigated the diversity, community composition, and abundance of anaerobic ammonium oxidation (anammox) bacteria along the altitudinal gradient in Qinghai–Tibet Plateau. Two types of soil samples (wetland and dryland soils, n = 123) were collected from 641 m to 5,033 m altitudes. Polym...

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Autores principales: Zhao, Siyan, Zhuang, Linjie, Wang, Cheng, Li, Yifei, Wang, Shanyun, Zhu, Guibing
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5911990/
https://www.ncbi.nlm.nih.gov/pubmed/29285891
http://dx.doi.org/10.1002/mbo3.556
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author Zhao, Siyan
Zhuang, Linjie
Wang, Cheng
Li, Yifei
Wang, Shanyun
Zhu, Guibing
author_facet Zhao, Siyan
Zhuang, Linjie
Wang, Cheng
Li, Yifei
Wang, Shanyun
Zhu, Guibing
author_sort Zhao, Siyan
collection PubMed
description This study investigated the diversity, community composition, and abundance of anaerobic ammonium oxidation (anammox) bacteria along the altitudinal gradient in Qinghai–Tibet Plateau. Two types of soil samples (wetland and dryland soils, n = 123) were collected from 641 m to 5,033 m altitudes. Polymerase chain reaction (PCR) screening showed that anammox were not widespread, and were only detected in 9 sampling sites of the 50 sites tested by amplifying the 16S rRNA genes. Then, only samples collected from Linzhi (2,715 m), Rikaze (4,030 m), and Naqu (5,011 m), which were positive for the presence of anammox, were further processed to explore the biogeography of anammox bacteria in Qinghai–Tibet Plateau. Results of high‐throughput sequencing targeting the hydrazine synthesis β‐subunit (hzsB) gene revealed the presence of three known anammox genera (Candidatus Brocadia, Candidatus Jettenia, and Candidatus Kuenenia) in both soil types. Their diversity, community composition, and abundance did not show significant variation with altitude at large scale. However, it was the small‐scale environmental heterogeneities between wetland and dryland soils that determined their biogeographical distribution. Specifically, the dryland soils had higher diversity of anammox bacteria than the wetland soils, but their abundance patterns varied. The community composition of anammox bacteria were found to be influenced by soil nitrate content.
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spelling pubmed-59119902018-05-02 High‐throughput analysis of anammox bacteria in wetland and dryland soils along the altitudinal gradient in Qinghai–Tibet Plateau Zhao, Siyan Zhuang, Linjie Wang, Cheng Li, Yifei Wang, Shanyun Zhu, Guibing Microbiologyopen Original Research This study investigated the diversity, community composition, and abundance of anaerobic ammonium oxidation (anammox) bacteria along the altitudinal gradient in Qinghai–Tibet Plateau. Two types of soil samples (wetland and dryland soils, n = 123) were collected from 641 m to 5,033 m altitudes. Polymerase chain reaction (PCR) screening showed that anammox were not widespread, and were only detected in 9 sampling sites of the 50 sites tested by amplifying the 16S rRNA genes. Then, only samples collected from Linzhi (2,715 m), Rikaze (4,030 m), and Naqu (5,011 m), which were positive for the presence of anammox, were further processed to explore the biogeography of anammox bacteria in Qinghai–Tibet Plateau. Results of high‐throughput sequencing targeting the hydrazine synthesis β‐subunit (hzsB) gene revealed the presence of three known anammox genera (Candidatus Brocadia, Candidatus Jettenia, and Candidatus Kuenenia) in both soil types. Their diversity, community composition, and abundance did not show significant variation with altitude at large scale. However, it was the small‐scale environmental heterogeneities between wetland and dryland soils that determined their biogeographical distribution. Specifically, the dryland soils had higher diversity of anammox bacteria than the wetland soils, but their abundance patterns varied. The community composition of anammox bacteria were found to be influenced by soil nitrate content. John Wiley and Sons Inc. 2017-12-29 /pmc/articles/PMC5911990/ /pubmed/29285891 http://dx.doi.org/10.1002/mbo3.556 Text en © 2017 The Authors. MicrobiologyOpen published by John Wiley & Sons Ltd. This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Research
Zhao, Siyan
Zhuang, Linjie
Wang, Cheng
Li, Yifei
Wang, Shanyun
Zhu, Guibing
High‐throughput analysis of anammox bacteria in wetland and dryland soils along the altitudinal gradient in Qinghai–Tibet Plateau
title High‐throughput analysis of anammox bacteria in wetland and dryland soils along the altitudinal gradient in Qinghai–Tibet Plateau
title_full High‐throughput analysis of anammox bacteria in wetland and dryland soils along the altitudinal gradient in Qinghai–Tibet Plateau
title_fullStr High‐throughput analysis of anammox bacteria in wetland and dryland soils along the altitudinal gradient in Qinghai–Tibet Plateau
title_full_unstemmed High‐throughput analysis of anammox bacteria in wetland and dryland soils along the altitudinal gradient in Qinghai–Tibet Plateau
title_short High‐throughput analysis of anammox bacteria in wetland and dryland soils along the altitudinal gradient in Qinghai–Tibet Plateau
title_sort high‐throughput analysis of anammox bacteria in wetland and dryland soils along the altitudinal gradient in qinghai–tibet plateau
topic Original Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5911990/
https://www.ncbi.nlm.nih.gov/pubmed/29285891
http://dx.doi.org/10.1002/mbo3.556
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