Cargando…
Molecular processes underlying synergistic linuron mineralization in a triple‐species bacterial consortium biofilm revealed by differential transcriptomics
The proteobacteria Variovorax sp. WDL1, Comamonas testosteroni WDL7, and Hyphomicrobium sulfonivorans WDL6 compose a triple‐species consortium that synergistically degrades and grows on the phenylurea herbicide linuron. To acquire a better insight into the interactions between the consortium members...
Autores principales: | , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
John Wiley and Sons Inc.
2018
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5911999/ https://www.ncbi.nlm.nih.gov/pubmed/29314727 http://dx.doi.org/10.1002/mbo3.559 |
_version_ | 1783316314684653568 |
---|---|
author | Albers, Pieter Weytjens, Bram De Mot, René Marchal, Kathleen Springael, Dirk |
author_facet | Albers, Pieter Weytjens, Bram De Mot, René Marchal, Kathleen Springael, Dirk |
author_sort | Albers, Pieter |
collection | PubMed |
description | The proteobacteria Variovorax sp. WDL1, Comamonas testosteroni WDL7, and Hyphomicrobium sulfonivorans WDL6 compose a triple‐species consortium that synergistically degrades and grows on the phenylurea herbicide linuron. To acquire a better insight into the interactions between the consortium members and the underlying molecular mechanisms, we compared the transcriptomes of the key biodegrading strains WDL7 and WDL1 grown as biofilms in either isolation or consortium conditions by differential RNAseq analysis. Differentially expressed pathways and cellular systems were inferred using the network‐based algorithm PheNetic. Coculturing affected mainly metabolism in WDL1. Significantly enhanced expression of hylA encoding linuron hydrolase was observed. Moreover, differential expression of several pathways involved in carbohydrate, amino acid, nitrogen, and sulfur metabolism was observed indicating that WDL1 gains carbon and energy from linuron indirectly by consuming excretion products from WDL7 and/or WDL6. Moreover, in consortium conditions, WDL1 showed a pronounced stress response and overexpression of cell to cell interaction systems such as quorum sensing, contact‐dependent inhibition, and Type VI secretion. Since the latter two systems can mediate interference competition, it prompts the question if synergistic linuron degradation is the result of true adaptive cooperation or rather a facultative interaction between bacteria that coincidentally occupy complementary metabolic niches. |
format | Online Article Text |
id | pubmed-5911999 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | John Wiley and Sons Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-59119992018-05-02 Molecular processes underlying synergistic linuron mineralization in a triple‐species bacterial consortium biofilm revealed by differential transcriptomics Albers, Pieter Weytjens, Bram De Mot, René Marchal, Kathleen Springael, Dirk Microbiologyopen Original Research The proteobacteria Variovorax sp. WDL1, Comamonas testosteroni WDL7, and Hyphomicrobium sulfonivorans WDL6 compose a triple‐species consortium that synergistically degrades and grows on the phenylurea herbicide linuron. To acquire a better insight into the interactions between the consortium members and the underlying molecular mechanisms, we compared the transcriptomes of the key biodegrading strains WDL7 and WDL1 grown as biofilms in either isolation or consortium conditions by differential RNAseq analysis. Differentially expressed pathways and cellular systems were inferred using the network‐based algorithm PheNetic. Coculturing affected mainly metabolism in WDL1. Significantly enhanced expression of hylA encoding linuron hydrolase was observed. Moreover, differential expression of several pathways involved in carbohydrate, amino acid, nitrogen, and sulfur metabolism was observed indicating that WDL1 gains carbon and energy from linuron indirectly by consuming excretion products from WDL7 and/or WDL6. Moreover, in consortium conditions, WDL1 showed a pronounced stress response and overexpression of cell to cell interaction systems such as quorum sensing, contact‐dependent inhibition, and Type VI secretion. Since the latter two systems can mediate interference competition, it prompts the question if synergistic linuron degradation is the result of true adaptive cooperation or rather a facultative interaction between bacteria that coincidentally occupy complementary metabolic niches. John Wiley and Sons Inc. 2018-01-03 /pmc/articles/PMC5911999/ /pubmed/29314727 http://dx.doi.org/10.1002/mbo3.559 Text en © 2018 The Authors. MicrobiologyOpen published by John Wiley & Sons Ltd. This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Original Research Albers, Pieter Weytjens, Bram De Mot, René Marchal, Kathleen Springael, Dirk Molecular processes underlying synergistic linuron mineralization in a triple‐species bacterial consortium biofilm revealed by differential transcriptomics |
title | Molecular processes underlying synergistic linuron mineralization in a triple‐species bacterial consortium biofilm revealed by differential transcriptomics |
title_full | Molecular processes underlying synergistic linuron mineralization in a triple‐species bacterial consortium biofilm revealed by differential transcriptomics |
title_fullStr | Molecular processes underlying synergistic linuron mineralization in a triple‐species bacterial consortium biofilm revealed by differential transcriptomics |
title_full_unstemmed | Molecular processes underlying synergistic linuron mineralization in a triple‐species bacterial consortium biofilm revealed by differential transcriptomics |
title_short | Molecular processes underlying synergistic linuron mineralization in a triple‐species bacterial consortium biofilm revealed by differential transcriptomics |
title_sort | molecular processes underlying synergistic linuron mineralization in a triple‐species bacterial consortium biofilm revealed by differential transcriptomics |
topic | Original Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5911999/ https://www.ncbi.nlm.nih.gov/pubmed/29314727 http://dx.doi.org/10.1002/mbo3.559 |
work_keys_str_mv | AT alberspieter molecularprocessesunderlyingsynergisticlinuronmineralizationinatriplespeciesbacterialconsortiumbiofilmrevealedbydifferentialtranscriptomics AT weytjensbram molecularprocessesunderlyingsynergisticlinuronmineralizationinatriplespeciesbacterialconsortiumbiofilmrevealedbydifferentialtranscriptomics AT demotrene molecularprocessesunderlyingsynergisticlinuronmineralizationinatriplespeciesbacterialconsortiumbiofilmrevealedbydifferentialtranscriptomics AT marchalkathleen molecularprocessesunderlyingsynergisticlinuronmineralizationinatriplespeciesbacterialconsortiumbiofilmrevealedbydifferentialtranscriptomics AT springaeldirk molecularprocessesunderlyingsynergisticlinuronmineralizationinatriplespeciesbacterialconsortiumbiofilmrevealedbydifferentialtranscriptomics |