Essential Metal Uptake in Gram-negative Bacteria: X-ray Fluorescence, Radioisotopes, and Cell Fractionation

We demonstrate a scalable method for the separation of the bacterial periplasm from the cytoplasm. This method is used to purify periplasmic protein for the purpose of biophysical characterization, and measure substrate transfer between periplasmic and cytoplasmic compartments. By carefully limiting...

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Detalles Bibliográficos
Autores principales: Radka, Christopher D., Radford, Lauren L., Massicano, Adriana V.F., DeLucas, Lawrence J., Lapi, Suzanne E., Aller, Stephen G.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MyJove Corporation 2018
Materias:
Chemistry
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5912328/
https://www.ncbi.nlm.nih.gov/pubmed/29443084
http://dx.doi.org/10.3791/57169

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5912328/
https://www.ncbi.nlm.nih.gov/pubmed/29443084
http://dx.doi.org/10.3791/57169

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