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Characterization and evaluation of graphene oxide scaffold for periodontal wound healing of class II furcation defects in dog
INTRODUCTION: The 3-dimensional scaffold plays a key role in volume and quality of repair tissue in periodontal tissue engineering therapy. We fabricated a novel 3D collagen scaffold containing carbon-based 2-dimensional layered material, named graphene oxide (GO). The aim of this study was to chara...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Dove Medical Press
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5912619/ https://www.ncbi.nlm.nih.gov/pubmed/29713167 http://dx.doi.org/10.2147/IJN.S163206 |
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author | Kawamoto, Kohei Miyaji, Hirofumi Nishida, Erika Miyata, Saori Kato, Akihito Tateyama, Akito Furihata, Tomokazu Shitomi, Kanako Iwanaga, Toshihiko Sugaya, Tsutomu |
author_facet | Kawamoto, Kohei Miyaji, Hirofumi Nishida, Erika Miyata, Saori Kato, Akihito Tateyama, Akito Furihata, Tomokazu Shitomi, Kanako Iwanaga, Toshihiko Sugaya, Tsutomu |
author_sort | Kawamoto, Kohei |
collection | PubMed |
description | INTRODUCTION: The 3-dimensional scaffold plays a key role in volume and quality of repair tissue in periodontal tissue engineering therapy. We fabricated a novel 3D collagen scaffold containing carbon-based 2-dimensional layered material, named graphene oxide (GO). The aim of this study was to characterize and assess GO scaffold for periodontal tissue healing of class II furcation defects in dog. MATERIALS AND METHODS: GO scaffolds were prepared by coating the surface of a 3D collagen sponge scaffold with GO dispersion. Scaffolds were characterized using cytotoxicity and tissue reactivity tests. In addition, GO scaffold was implanted into dog class II furcation defects and periodontal healing was investigated at 4 weeks postsurgery. RESULTS: GO scaffold exhibited low cytotoxicity and enhanced cellular ingrowth behavior and rat bone forming ability. In addition, GO scaffold stimulated healing of dog class II furcation defects. Periodontal attachment formation, including alveolar bone, periodontal ligament-like tissue, and cementum-like tissue, was significantly increased by GO scaffold implantation, compared with untreated scaffold. CONCLUSION: The results suggest that GO scaffold is biocompatible and possesses excellent bone and periodontal tissue formation ability. Therefore, GO scaffold would be beneficial for periodontal tissue engineering therapy. |
format | Online Article Text |
id | pubmed-5912619 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Dove Medical Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-59126192018-04-30 Characterization and evaluation of graphene oxide scaffold for periodontal wound healing of class II furcation defects in dog Kawamoto, Kohei Miyaji, Hirofumi Nishida, Erika Miyata, Saori Kato, Akihito Tateyama, Akito Furihata, Tomokazu Shitomi, Kanako Iwanaga, Toshihiko Sugaya, Tsutomu Int J Nanomedicine Original Research INTRODUCTION: The 3-dimensional scaffold plays a key role in volume and quality of repair tissue in periodontal tissue engineering therapy. We fabricated a novel 3D collagen scaffold containing carbon-based 2-dimensional layered material, named graphene oxide (GO). The aim of this study was to characterize and assess GO scaffold for periodontal tissue healing of class II furcation defects in dog. MATERIALS AND METHODS: GO scaffolds were prepared by coating the surface of a 3D collagen sponge scaffold with GO dispersion. Scaffolds were characterized using cytotoxicity and tissue reactivity tests. In addition, GO scaffold was implanted into dog class II furcation defects and periodontal healing was investigated at 4 weeks postsurgery. RESULTS: GO scaffold exhibited low cytotoxicity and enhanced cellular ingrowth behavior and rat bone forming ability. In addition, GO scaffold stimulated healing of dog class II furcation defects. Periodontal attachment formation, including alveolar bone, periodontal ligament-like tissue, and cementum-like tissue, was significantly increased by GO scaffold implantation, compared with untreated scaffold. CONCLUSION: The results suggest that GO scaffold is biocompatible and possesses excellent bone and periodontal tissue formation ability. Therefore, GO scaffold would be beneficial for periodontal tissue engineering therapy. Dove Medical Press 2018-04-18 /pmc/articles/PMC5912619/ /pubmed/29713167 http://dx.doi.org/10.2147/IJN.S163206 Text en © 2018 Kawamoto et al. This work is published and licensed by Dove Medical Press Limited The full terms of this license are available at https://www.dovepress.com/terms.php and incorporate the Creative Commons Attribution – Non Commercial (unported, v3.0) License (http://creativecommons.org/licenses/by-nc/3.0/). By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed. |
spellingShingle | Original Research Kawamoto, Kohei Miyaji, Hirofumi Nishida, Erika Miyata, Saori Kato, Akihito Tateyama, Akito Furihata, Tomokazu Shitomi, Kanako Iwanaga, Toshihiko Sugaya, Tsutomu Characterization and evaluation of graphene oxide scaffold for periodontal wound healing of class II furcation defects in dog |
title | Characterization and evaluation of graphene oxide scaffold for periodontal wound healing of class II furcation defects in dog |
title_full | Characterization and evaluation of graphene oxide scaffold for periodontal wound healing of class II furcation defects in dog |
title_fullStr | Characterization and evaluation of graphene oxide scaffold for periodontal wound healing of class II furcation defects in dog |
title_full_unstemmed | Characterization and evaluation of graphene oxide scaffold for periodontal wound healing of class II furcation defects in dog |
title_short | Characterization and evaluation of graphene oxide scaffold for periodontal wound healing of class II furcation defects in dog |
title_sort | characterization and evaluation of graphene oxide scaffold for periodontal wound healing of class ii furcation defects in dog |
topic | Original Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5912619/ https://www.ncbi.nlm.nih.gov/pubmed/29713167 http://dx.doi.org/10.2147/IJN.S163206 |
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