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Suppression of the pelo protein by Wolbachia and its effect on dengue virus in Aedes aegypti

The endosymbiont Wolbachia is known to block replication of several important arboviruses, including dengue virus (DENV), in the mosquito vector Aedes aegypti. So far, the exact mechanism of this viral inhibition is not fully understood. A recent study in Drosophila melanogaster has demonstrated an...

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Autores principales: Asad, Sultan, Hussain, Mazhar, Hugo, Leon, Osei-Amo, Solomon, Zhang, Guangmei, Watterson, Daniel, Asgari, Sassan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5912784/
https://www.ncbi.nlm.nih.gov/pubmed/29641562
http://dx.doi.org/10.1371/journal.pntd.0006405
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author Asad, Sultan
Hussain, Mazhar
Hugo, Leon
Osei-Amo, Solomon
Zhang, Guangmei
Watterson, Daniel
Asgari, Sassan
author_facet Asad, Sultan
Hussain, Mazhar
Hugo, Leon
Osei-Amo, Solomon
Zhang, Guangmei
Watterson, Daniel
Asgari, Sassan
author_sort Asad, Sultan
collection PubMed
description The endosymbiont Wolbachia is known to block replication of several important arboviruses, including dengue virus (DENV), in the mosquito vector Aedes aegypti. So far, the exact mechanism of this viral inhibition is not fully understood. A recent study in Drosophila melanogaster has demonstrated an interaction between the pelo gene and Drosophila C virus. In this study, we explored the possible involvement of the pelo protein, that is involved in protein translation, in Wolbachia-mediated antiviral response and mosquito-DENV interaction. We found that pelo is upregulated during DENV replication and its silencing leads to reduced DENV virion production suggesting that it facilities DENV replication. However, in the presence of Wolbachia, specifically in female mosquitoes, the pelo protein is downregulated and its subcellular localization is altered, which could contribute to reduction in DENV replication in Ae. aegypti. In addition, we show that the microRNA aae-miR-2940-5p, whose abundance is highly enriched in Wolbachia-infected mosquitoes, might mediate regulation of pelo. Our data reveals identification of pelo as a host factor that is positively involved in DENV replication, and its suppression in the presence of Wolbachia may contribute to virus blocking exhibited by the endosymbiont.
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spelling pubmed-59127842018-05-04 Suppression of the pelo protein by Wolbachia and its effect on dengue virus in Aedes aegypti Asad, Sultan Hussain, Mazhar Hugo, Leon Osei-Amo, Solomon Zhang, Guangmei Watterson, Daniel Asgari, Sassan PLoS Negl Trop Dis Research Article The endosymbiont Wolbachia is known to block replication of several important arboviruses, including dengue virus (DENV), in the mosquito vector Aedes aegypti. So far, the exact mechanism of this viral inhibition is not fully understood. A recent study in Drosophila melanogaster has demonstrated an interaction between the pelo gene and Drosophila C virus. In this study, we explored the possible involvement of the pelo protein, that is involved in protein translation, in Wolbachia-mediated antiviral response and mosquito-DENV interaction. We found that pelo is upregulated during DENV replication and its silencing leads to reduced DENV virion production suggesting that it facilities DENV replication. However, in the presence of Wolbachia, specifically in female mosquitoes, the pelo protein is downregulated and its subcellular localization is altered, which could contribute to reduction in DENV replication in Ae. aegypti. In addition, we show that the microRNA aae-miR-2940-5p, whose abundance is highly enriched in Wolbachia-infected mosquitoes, might mediate regulation of pelo. Our data reveals identification of pelo as a host factor that is positively involved in DENV replication, and its suppression in the presence of Wolbachia may contribute to virus blocking exhibited by the endosymbiont. Public Library of Science 2018-04-11 /pmc/articles/PMC5912784/ /pubmed/29641562 http://dx.doi.org/10.1371/journal.pntd.0006405 Text en © 2018 Asad et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Asad, Sultan
Hussain, Mazhar
Hugo, Leon
Osei-Amo, Solomon
Zhang, Guangmei
Watterson, Daniel
Asgari, Sassan
Suppression of the pelo protein by Wolbachia and its effect on dengue virus in Aedes aegypti
title Suppression of the pelo protein by Wolbachia and its effect on dengue virus in Aedes aegypti
title_full Suppression of the pelo protein by Wolbachia and its effect on dengue virus in Aedes aegypti
title_fullStr Suppression of the pelo protein by Wolbachia and its effect on dengue virus in Aedes aegypti
title_full_unstemmed Suppression of the pelo protein by Wolbachia and its effect on dengue virus in Aedes aegypti
title_short Suppression of the pelo protein by Wolbachia and its effect on dengue virus in Aedes aegypti
title_sort suppression of the pelo protein by wolbachia and its effect on dengue virus in aedes aegypti
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5912784/
https://www.ncbi.nlm.nih.gov/pubmed/29641562
http://dx.doi.org/10.1371/journal.pntd.0006405
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