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Transcriptome Analysis Reveals genes involved in flavonoid biosynthesis and accumulation in Dendrobium catenatum From Different Locations

In this study, we applied transcriptome and UHPLC-MS technologies to investigate the flavonoids and their biosynthesis- and accumulation-related genes in Dendrobium catenatum from three different locations. Eight flavonoid glycosides were identified using standard references or previously isolated s...

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Autores principales: Lei, Zhouxi, Zhou, Chunhua, Ji, Xiaoyu, Wei, Gang, Huang, Yuechun, Yu, Wenxia, Luo, Yingyi, Qiu, Yue
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5913234/
https://www.ncbi.nlm.nih.gov/pubmed/29686299
http://dx.doi.org/10.1038/s41598-018-24751-y
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author Lei, Zhouxi
Zhou, Chunhua
Ji, Xiaoyu
Wei, Gang
Huang, Yuechun
Yu, Wenxia
Luo, Yingyi
Qiu, Yue
author_facet Lei, Zhouxi
Zhou, Chunhua
Ji, Xiaoyu
Wei, Gang
Huang, Yuechun
Yu, Wenxia
Luo, Yingyi
Qiu, Yue
author_sort Lei, Zhouxi
collection PubMed
description In this study, we applied transcriptome and UHPLC-MS technologies to investigate the flavonoids and their biosynthesis- and accumulation-related genes in Dendrobium catenatum from three different locations. Eight flavonoid glycosides were identified using standard references or previously isolated substances with MS data analysis. The total flavonoid contents were determined by reagents, and all the data were analyzed. In total, 23139 unigenes were obtained using the Dendrobium catenatum genome data. Of these, 10398 were annotated in the Gene Ontology (GO) database, 4203 were annotated in the KEGG database, and 10917 were annotated in the EuKaryotic Orthologous Groups (KOG) database. Thirty-one of the unigenes annotated by the KEGG database were involved in flavonoid pathways. The genes involved in bio-modification, accumulation, transportation and the regulation of the flavonoid bio-synthesis process were investigated. In conclusion, the flavonoids in Dendrobium catenatum from three different locations were different in quantitative and qualitative which may contribute to the establishment of quality control method for this herbal plant. These differences were determined by flavonoids biosynthesis process and they were concluded by sorting out the expression level of certain biosynthesis related genes.
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spelling pubmed-59132342018-04-30 Transcriptome Analysis Reveals genes involved in flavonoid biosynthesis and accumulation in Dendrobium catenatum From Different Locations Lei, Zhouxi Zhou, Chunhua Ji, Xiaoyu Wei, Gang Huang, Yuechun Yu, Wenxia Luo, Yingyi Qiu, Yue Sci Rep Article In this study, we applied transcriptome and UHPLC-MS technologies to investigate the flavonoids and their biosynthesis- and accumulation-related genes in Dendrobium catenatum from three different locations. Eight flavonoid glycosides were identified using standard references or previously isolated substances with MS data analysis. The total flavonoid contents were determined by reagents, and all the data were analyzed. In total, 23139 unigenes were obtained using the Dendrobium catenatum genome data. Of these, 10398 were annotated in the Gene Ontology (GO) database, 4203 were annotated in the KEGG database, and 10917 were annotated in the EuKaryotic Orthologous Groups (KOG) database. Thirty-one of the unigenes annotated by the KEGG database were involved in flavonoid pathways. The genes involved in bio-modification, accumulation, transportation and the regulation of the flavonoid bio-synthesis process were investigated. In conclusion, the flavonoids in Dendrobium catenatum from three different locations were different in quantitative and qualitative which may contribute to the establishment of quality control method for this herbal plant. These differences were determined by flavonoids biosynthesis process and they were concluded by sorting out the expression level of certain biosynthesis related genes. Nature Publishing Group UK 2018-04-23 /pmc/articles/PMC5913234/ /pubmed/29686299 http://dx.doi.org/10.1038/s41598-018-24751-y Text en © The Author(s) 2018 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Lei, Zhouxi
Zhou, Chunhua
Ji, Xiaoyu
Wei, Gang
Huang, Yuechun
Yu, Wenxia
Luo, Yingyi
Qiu, Yue
Transcriptome Analysis Reveals genes involved in flavonoid biosynthesis and accumulation in Dendrobium catenatum From Different Locations
title Transcriptome Analysis Reveals genes involved in flavonoid biosynthesis and accumulation in Dendrobium catenatum From Different Locations
title_full Transcriptome Analysis Reveals genes involved in flavonoid biosynthesis and accumulation in Dendrobium catenatum From Different Locations
title_fullStr Transcriptome Analysis Reveals genes involved in flavonoid biosynthesis and accumulation in Dendrobium catenatum From Different Locations
title_full_unstemmed Transcriptome Analysis Reveals genes involved in flavonoid biosynthesis and accumulation in Dendrobium catenatum From Different Locations
title_short Transcriptome Analysis Reveals genes involved in flavonoid biosynthesis and accumulation in Dendrobium catenatum From Different Locations
title_sort transcriptome analysis reveals genes involved in flavonoid biosynthesis and accumulation in dendrobium catenatum from different locations
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5913234/
https://www.ncbi.nlm.nih.gov/pubmed/29686299
http://dx.doi.org/10.1038/s41598-018-24751-y
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