Cargando…

Micro RNAs from DNA Viruses are Found Widely in Plasma in a Large Observational Human Population

Viral infections associate with disease risk and select families of viruses encode miRNAs that control an efficient viral cycle. The association of viral miRNA expression with disease in a large human population has not been previously explored. We sequenced plasma RNA from 40 participants of the Fr...

Descripción completa

Detalles Bibliográficos
Autores principales: Koupenova, Milka, Mick, Eric, Corkrey, Heather A., Huan, Tianxiao, Clancy, Lauren, Shah, Ravi, Benjamin, Emelia J., Levy, Daniel, Kurt-Jones, Evelyn A., Tanriverdi, Kahraman, Freedman, Jane E.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5913337/
https://www.ncbi.nlm.nih.gov/pubmed/29686252
http://dx.doi.org/10.1038/s41598-018-24765-6
_version_ 1783316522040557568
author Koupenova, Milka
Mick, Eric
Corkrey, Heather A.
Huan, Tianxiao
Clancy, Lauren
Shah, Ravi
Benjamin, Emelia J.
Levy, Daniel
Kurt-Jones, Evelyn A.
Tanriverdi, Kahraman
Freedman, Jane E.
author_facet Koupenova, Milka
Mick, Eric
Corkrey, Heather A.
Huan, Tianxiao
Clancy, Lauren
Shah, Ravi
Benjamin, Emelia J.
Levy, Daniel
Kurt-Jones, Evelyn A.
Tanriverdi, Kahraman
Freedman, Jane E.
author_sort Koupenova, Milka
collection PubMed
description Viral infections associate with disease risk and select families of viruses encode miRNAs that control an efficient viral cycle. The association of viral miRNA expression with disease in a large human population has not been previously explored. We sequenced plasma RNA from 40 participants of the Framingham Heart Study (FHS, Offspring Cohort, Visit 8) and identified 3 viral miRNAs from 3 different human Herpesviridae. These miRNAs were mostly related to viral latency and have not been previously detected in human plasma. Viral miRNA expression was then screened in the plasma of 2763 participants of the remaining cohort utilizing high-throughput RT-qPCR. All 3 viral miRNAs associated with combinations of inflammatory or prothrombotic circulating biomarkers (sTNFRII, IL-6, sICAM1, OPG, P-selectin) but did not associate with hypertension, coronary heart disease or cancer. Using a large observational population, we demonstrate that the presence of select viral miRNAs in the human circulation associate with inflammatory biomarkers and possibly immune response, but fail to associate with overt disease. This study greatly extends smaller singular observations of viral miRNAs in the human circulation and suggests that select viral miRNAs, such as those for latency, may not impact disease manifestation.
format Online
Article
Text
id pubmed-5913337
institution National Center for Biotechnology Information
language English
publishDate 2018
publisher Nature Publishing Group UK
record_format MEDLINE/PubMed
spelling pubmed-59133372018-04-30 Micro RNAs from DNA Viruses are Found Widely in Plasma in a Large Observational Human Population Koupenova, Milka Mick, Eric Corkrey, Heather A. Huan, Tianxiao Clancy, Lauren Shah, Ravi Benjamin, Emelia J. Levy, Daniel Kurt-Jones, Evelyn A. Tanriverdi, Kahraman Freedman, Jane E. Sci Rep Article Viral infections associate with disease risk and select families of viruses encode miRNAs that control an efficient viral cycle. The association of viral miRNA expression with disease in a large human population has not been previously explored. We sequenced plasma RNA from 40 participants of the Framingham Heart Study (FHS, Offspring Cohort, Visit 8) and identified 3 viral miRNAs from 3 different human Herpesviridae. These miRNAs were mostly related to viral latency and have not been previously detected in human plasma. Viral miRNA expression was then screened in the plasma of 2763 participants of the remaining cohort utilizing high-throughput RT-qPCR. All 3 viral miRNAs associated with combinations of inflammatory or prothrombotic circulating biomarkers (sTNFRII, IL-6, sICAM1, OPG, P-selectin) but did not associate with hypertension, coronary heart disease or cancer. Using a large observational population, we demonstrate that the presence of select viral miRNAs in the human circulation associate with inflammatory biomarkers and possibly immune response, but fail to associate with overt disease. This study greatly extends smaller singular observations of viral miRNAs in the human circulation and suggests that select viral miRNAs, such as those for latency, may not impact disease manifestation. Nature Publishing Group UK 2018-04-23 /pmc/articles/PMC5913337/ /pubmed/29686252 http://dx.doi.org/10.1038/s41598-018-24765-6 Text en © The Author(s) 2018 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Koupenova, Milka
Mick, Eric
Corkrey, Heather A.
Huan, Tianxiao
Clancy, Lauren
Shah, Ravi
Benjamin, Emelia J.
Levy, Daniel
Kurt-Jones, Evelyn A.
Tanriverdi, Kahraman
Freedman, Jane E.
Micro RNAs from DNA Viruses are Found Widely in Plasma in a Large Observational Human Population
title Micro RNAs from DNA Viruses are Found Widely in Plasma in a Large Observational Human Population
title_full Micro RNAs from DNA Viruses are Found Widely in Plasma in a Large Observational Human Population
title_fullStr Micro RNAs from DNA Viruses are Found Widely in Plasma in a Large Observational Human Population
title_full_unstemmed Micro RNAs from DNA Viruses are Found Widely in Plasma in a Large Observational Human Population
title_short Micro RNAs from DNA Viruses are Found Widely in Plasma in a Large Observational Human Population
title_sort micro rnas from dna viruses are found widely in plasma in a large observational human population
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5913337/
https://www.ncbi.nlm.nih.gov/pubmed/29686252
http://dx.doi.org/10.1038/s41598-018-24765-6
work_keys_str_mv AT koupenovamilka micrornasfromdnavirusesarefoundwidelyinplasmainalargeobservationalhumanpopulation
AT mickeric micrornasfromdnavirusesarefoundwidelyinplasmainalargeobservationalhumanpopulation
AT corkreyheathera micrornasfromdnavirusesarefoundwidelyinplasmainalargeobservationalhumanpopulation
AT huantianxiao micrornasfromdnavirusesarefoundwidelyinplasmainalargeobservationalhumanpopulation
AT clancylauren micrornasfromdnavirusesarefoundwidelyinplasmainalargeobservationalhumanpopulation
AT shahravi micrornasfromdnavirusesarefoundwidelyinplasmainalargeobservationalhumanpopulation
AT benjaminemeliaj micrornasfromdnavirusesarefoundwidelyinplasmainalargeobservationalhumanpopulation
AT levydaniel micrornasfromdnavirusesarefoundwidelyinplasmainalargeobservationalhumanpopulation
AT kurtjonesevelyna micrornasfromdnavirusesarefoundwidelyinplasmainalargeobservationalhumanpopulation
AT tanriverdikahraman micrornasfromdnavirusesarefoundwidelyinplasmainalargeobservationalhumanpopulation
AT freedmanjanee micrornasfromdnavirusesarefoundwidelyinplasmainalargeobservationalhumanpopulation