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Expression of MdCCD7 in the scion determines the extent of sylleptic branching and the primary shoot growth rate of apple trees

Branching has a major influence on the overall shape and productivity of a plant. Strigolactones (SLs) have been identified as plant hormones that have a key role in suppressing the outgrowth of axillary meristems. CAROTENOID CLEAVAGE DIOXYGENASE (CCD) genes are integral to the biosynthesis of SLs a...

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Autores principales: Foster, Toshi M, Ledger, Susan E, Janssen, Bart J, Luo, Zhiwei, Drummond, Revel S M, Tomes, Sumathi, Karunairetnam, Sakuntala, Waite, Chethi N, Funnell, Keith A, van Hooijdonk, Ben M, Saei, Ali, Seleznyova, Alla N, Snowden, Kimberley C
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5913623/
https://www.ncbi.nlm.nih.gov/pubmed/29190381
http://dx.doi.org/10.1093/jxb/erx404
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author Foster, Toshi M
Ledger, Susan E
Janssen, Bart J
Luo, Zhiwei
Drummond, Revel S M
Tomes, Sumathi
Karunairetnam, Sakuntala
Waite, Chethi N
Funnell, Keith A
van Hooijdonk, Ben M
Saei, Ali
Seleznyova, Alla N
Snowden, Kimberley C
author_facet Foster, Toshi M
Ledger, Susan E
Janssen, Bart J
Luo, Zhiwei
Drummond, Revel S M
Tomes, Sumathi
Karunairetnam, Sakuntala
Waite, Chethi N
Funnell, Keith A
van Hooijdonk, Ben M
Saei, Ali
Seleznyova, Alla N
Snowden, Kimberley C
author_sort Foster, Toshi M
collection PubMed
description Branching has a major influence on the overall shape and productivity of a plant. Strigolactones (SLs) have been identified as plant hormones that have a key role in suppressing the outgrowth of axillary meristems. CAROTENOID CLEAVAGE DIOXYGENASE (CCD) genes are integral to the biosynthesis of SLs and are well characterized in annual plants, but their role in woody perennials is relatively unknown. We identified CCD7 and CCD8 orthologues from apple and demonstrated that MdCCD7 and MdCCD8 are able to complement the Arabidopsis branching mutants max3 and max4 respectively, indicating conserved function. RNAi lines of MdCCD7 show reduced gene expression and increased branching in apple. We performed reciprocal grafting experiments with combinations of MdCCD7 RNAi and wild-type ‘Royal Gala’ as rootstocks and scion. Unexpectedly, wild-type roots were unable to suppress branching in MdCCD7 RNAi scions. Another key finding was that MdCCD7 RNAi scions initiated phytomers at an increased rate relative to the wild type, resulting in a greater node number and primary shoot length. We suggest that localized SL biosynthesis in the shoot, rather than roots, controls axillary bud outgrowth and shoot growth rate in apple.
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spelling pubmed-59136232018-04-30 Expression of MdCCD7 in the scion determines the extent of sylleptic branching and the primary shoot growth rate of apple trees Foster, Toshi M Ledger, Susan E Janssen, Bart J Luo, Zhiwei Drummond, Revel S M Tomes, Sumathi Karunairetnam, Sakuntala Waite, Chethi N Funnell, Keith A van Hooijdonk, Ben M Saei, Ali Seleznyova, Alla N Snowden, Kimberley C J Exp Bot Research Papers Branching has a major influence on the overall shape and productivity of a plant. Strigolactones (SLs) have been identified as plant hormones that have a key role in suppressing the outgrowth of axillary meristems. CAROTENOID CLEAVAGE DIOXYGENASE (CCD) genes are integral to the biosynthesis of SLs and are well characterized in annual plants, but their role in woody perennials is relatively unknown. We identified CCD7 and CCD8 orthologues from apple and demonstrated that MdCCD7 and MdCCD8 are able to complement the Arabidopsis branching mutants max3 and max4 respectively, indicating conserved function. RNAi lines of MdCCD7 show reduced gene expression and increased branching in apple. We performed reciprocal grafting experiments with combinations of MdCCD7 RNAi and wild-type ‘Royal Gala’ as rootstocks and scion. Unexpectedly, wild-type roots were unable to suppress branching in MdCCD7 RNAi scions. Another key finding was that MdCCD7 RNAi scions initiated phytomers at an increased rate relative to the wild type, resulting in a greater node number and primary shoot length. We suggest that localized SL biosynthesis in the shoot, rather than roots, controls axillary bud outgrowth and shoot growth rate in apple. Oxford University Press 2018-04-13 2017-11-28 /pmc/articles/PMC5913623/ /pubmed/29190381 http://dx.doi.org/10.1093/jxb/erx404 Text en © The Author(s) 2017. Published by Oxford University Press on behalf of the Society for Experimental Biology. http://creativecommons.org/licenses/by/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Papers
Foster, Toshi M
Ledger, Susan E
Janssen, Bart J
Luo, Zhiwei
Drummond, Revel S M
Tomes, Sumathi
Karunairetnam, Sakuntala
Waite, Chethi N
Funnell, Keith A
van Hooijdonk, Ben M
Saei, Ali
Seleznyova, Alla N
Snowden, Kimberley C
Expression of MdCCD7 in the scion determines the extent of sylleptic branching and the primary shoot growth rate of apple trees
title Expression of MdCCD7 in the scion determines the extent of sylleptic branching and the primary shoot growth rate of apple trees
title_full Expression of MdCCD7 in the scion determines the extent of sylleptic branching and the primary shoot growth rate of apple trees
title_fullStr Expression of MdCCD7 in the scion determines the extent of sylleptic branching and the primary shoot growth rate of apple trees
title_full_unstemmed Expression of MdCCD7 in the scion determines the extent of sylleptic branching and the primary shoot growth rate of apple trees
title_short Expression of MdCCD7 in the scion determines the extent of sylleptic branching and the primary shoot growth rate of apple trees
title_sort expression of mdccd7 in the scion determines the extent of sylleptic branching and the primary shoot growth rate of apple trees
topic Research Papers
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5913623/
https://www.ncbi.nlm.nih.gov/pubmed/29190381
http://dx.doi.org/10.1093/jxb/erx404
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