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Effects of n-3 fatty acids and exercise on oxidative stress parameters in type 2 diabetic: a randomized clinical trial

BACKGROUND: The relationship between diabetes and oxidative stress has been previously reported. Exercise represents a useful non-pharmacological strategy for the treatment in type 2 diabetic (T2DM) patients, but high intensity exercise can induce a transient inflammatory state and increase oxidativ...

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Autores principales: Fayh, Ana Paula Trussardi, Borges, Katiuce, Cunha, Giovani Santos, Krause, Mauricio, Rocha, Ricardo, de Bittencourt, Paulo Ivo Homem, Moreira, José Cláudio Fonseca, Friedman, Rogério, da Silva Rossato, Juliane, Fernandes, Jõao Roberto, Reischak-Oliveira, Alvaro
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5914016/
https://www.ncbi.nlm.nih.gov/pubmed/29713249
http://dx.doi.org/10.1186/s12970-018-0222-2
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author Fayh, Ana Paula Trussardi
Borges, Katiuce
Cunha, Giovani Santos
Krause, Mauricio
Rocha, Ricardo
de Bittencourt, Paulo Ivo Homem
Moreira, José Cláudio Fonseca
Friedman, Rogério
da Silva Rossato, Juliane
Fernandes, Jõao Roberto
Reischak-Oliveira, Alvaro
author_facet Fayh, Ana Paula Trussardi
Borges, Katiuce
Cunha, Giovani Santos
Krause, Mauricio
Rocha, Ricardo
de Bittencourt, Paulo Ivo Homem
Moreira, José Cláudio Fonseca
Friedman, Rogério
da Silva Rossato, Juliane
Fernandes, Jõao Roberto
Reischak-Oliveira, Alvaro
author_sort Fayh, Ana Paula Trussardi
collection PubMed
description BACKGROUND: The relationship between diabetes and oxidative stress has been previously reported. Exercise represents a useful non-pharmacological strategy for the treatment in type 2 diabetic (T2DM) patients, but high intensity exercise can induce a transient inflammatory state and increase oxidative stress. Nutritional strategies that may contribute to the reduction of oxidative stress induced by acute exercise are necessary. The aim of this study was to examine if n-3 PUFA supplementation intervention can attenuate the inflammatory response and oxidative stress associated with high intensity exercise in this population. As a primary outcome, lipoperoxidation measurements (TBARS and F2-isoprostanes) were selected. METHODS: Thirty T2DM patients, without chronic complications, were randomly allocated into two groups: placebo (gelatin capsules) or n-3 PUFA (capsules containing 180 mg of eicosapentaenoic acid and 120 mg of docosahexaenoic acid). Blood samples were collected fasting before and after 8 weeks supplementation. In the beginning and at the end of protocol, an acute exercise was performed (treadmill), and new blood samples were collected before and immediately after the exercise for measurements of oxidative stress and high-sensitivity C-reactive protein (hs-CRP). RESULTS: After the supplementation period, a decrease in triglycerides levels was observed only in n-3 PUFA supplementation group (mean difference and 95% CI of 0.002 (0.000–0.004), p = 0.005). Supplementation also significantly reduced TRAP levels after exercise (mean difference and 95% CI to 9641 (− 20,068–39,351) for − 33,884 (− 56,976 - -10,793), p = 0.004, Cohen’s d effect size = 1.12), but no significant difference was observed in n-3 PUFA supplementation group in lipoperoxidation parameters as TBARS (mean difference and 95% CI to − 3.8 (− 10–2.4) for − 2.9 (− 1.6–7.4) or F2-isoprostanes (mean difference and 95% CI -0.05 (− 0.19–0.10) for − 0.02 (− 0.19–0.16), p > 0.05 for both. CONCLUSION: PUFA n-3 supplementation reduced triglycerides as well as TRAP levels after exercise, without a significant effect on inflammatory and oxidative stress markers. This study is registered at ClinicalTrials.gov with the registration number of NCT03182712.
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spelling pubmed-59140162018-04-30 Effects of n-3 fatty acids and exercise on oxidative stress parameters in type 2 diabetic: a randomized clinical trial Fayh, Ana Paula Trussardi Borges, Katiuce Cunha, Giovani Santos Krause, Mauricio Rocha, Ricardo de Bittencourt, Paulo Ivo Homem Moreira, José Cláudio Fonseca Friedman, Rogério da Silva Rossato, Juliane Fernandes, Jõao Roberto Reischak-Oliveira, Alvaro J Int Soc Sports Nutr Research Article BACKGROUND: The relationship between diabetes and oxidative stress has been previously reported. Exercise represents a useful non-pharmacological strategy for the treatment in type 2 diabetic (T2DM) patients, but high intensity exercise can induce a transient inflammatory state and increase oxidative stress. Nutritional strategies that may contribute to the reduction of oxidative stress induced by acute exercise are necessary. The aim of this study was to examine if n-3 PUFA supplementation intervention can attenuate the inflammatory response and oxidative stress associated with high intensity exercise in this population. As a primary outcome, lipoperoxidation measurements (TBARS and F2-isoprostanes) were selected. METHODS: Thirty T2DM patients, without chronic complications, were randomly allocated into two groups: placebo (gelatin capsules) or n-3 PUFA (capsules containing 180 mg of eicosapentaenoic acid and 120 mg of docosahexaenoic acid). Blood samples were collected fasting before and after 8 weeks supplementation. In the beginning and at the end of protocol, an acute exercise was performed (treadmill), and new blood samples were collected before and immediately after the exercise for measurements of oxidative stress and high-sensitivity C-reactive protein (hs-CRP). RESULTS: After the supplementation period, a decrease in triglycerides levels was observed only in n-3 PUFA supplementation group (mean difference and 95% CI of 0.002 (0.000–0.004), p = 0.005). Supplementation also significantly reduced TRAP levels after exercise (mean difference and 95% CI to 9641 (− 20,068–39,351) for − 33,884 (− 56,976 - -10,793), p = 0.004, Cohen’s d effect size = 1.12), but no significant difference was observed in n-3 PUFA supplementation group in lipoperoxidation parameters as TBARS (mean difference and 95% CI to − 3.8 (− 10–2.4) for − 2.9 (− 1.6–7.4) or F2-isoprostanes (mean difference and 95% CI -0.05 (− 0.19–0.10) for − 0.02 (− 0.19–0.16), p > 0.05 for both. CONCLUSION: PUFA n-3 supplementation reduced triglycerides as well as TRAP levels after exercise, without a significant effect on inflammatory and oxidative stress markers. This study is registered at ClinicalTrials.gov with the registration number of NCT03182712. BioMed Central 2018-04-23 /pmc/articles/PMC5914016/ /pubmed/29713249 http://dx.doi.org/10.1186/s12970-018-0222-2 Text en © The Author(s). 2018 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research Article
Fayh, Ana Paula Trussardi
Borges, Katiuce
Cunha, Giovani Santos
Krause, Mauricio
Rocha, Ricardo
de Bittencourt, Paulo Ivo Homem
Moreira, José Cláudio Fonseca
Friedman, Rogério
da Silva Rossato, Juliane
Fernandes, Jõao Roberto
Reischak-Oliveira, Alvaro
Effects of n-3 fatty acids and exercise on oxidative stress parameters in type 2 diabetic: a randomized clinical trial
title Effects of n-3 fatty acids and exercise on oxidative stress parameters in type 2 diabetic: a randomized clinical trial
title_full Effects of n-3 fatty acids and exercise on oxidative stress parameters in type 2 diabetic: a randomized clinical trial
title_fullStr Effects of n-3 fatty acids and exercise on oxidative stress parameters in type 2 diabetic: a randomized clinical trial
title_full_unstemmed Effects of n-3 fatty acids and exercise on oxidative stress parameters in type 2 diabetic: a randomized clinical trial
title_short Effects of n-3 fatty acids and exercise on oxidative stress parameters in type 2 diabetic: a randomized clinical trial
title_sort effects of n-3 fatty acids and exercise on oxidative stress parameters in type 2 diabetic: a randomized clinical trial
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5914016/
https://www.ncbi.nlm.nih.gov/pubmed/29713249
http://dx.doi.org/10.1186/s12970-018-0222-2
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