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Effect of biogenic selenium nanoparticles on ERG11 and CDR1 gene expression in both fluconazole-resistant and -susceptible Candida albicans isolates

BACKGROUND AND PURPOSE: Candida albicans is the most common Candida species (sp.) isolated from fungal infections. Azole resistance in Candida species has been considerably increased in the last decades. Given the toxicity of the antimicrobial drugs, resistance to antifungal agents, and drug interac...

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Autores principales: Parsameher, Nasrin, Rezaei, Sassan, Khodavasiy, Sadegh, Salari, Samira, Hadizade, Sanaz, Kord, Mohammad, Ayatollahi Mousavi, Seyed Amin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Iranian Society of Medical Mycology 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5914922/
https://www.ncbi.nlm.nih.gov/pubmed/29707669
http://dx.doi.org/10.29252/cmm.3.3.16
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author Parsameher, Nasrin
Rezaei, Sassan
Khodavasiy, Sadegh
Salari, Samira
Hadizade, Sanaz
Kord, Mohammad
Ayatollahi Mousavi, Seyed Amin
author_facet Parsameher, Nasrin
Rezaei, Sassan
Khodavasiy, Sadegh
Salari, Samira
Hadizade, Sanaz
Kord, Mohammad
Ayatollahi Mousavi, Seyed Amin
author_sort Parsameher, Nasrin
collection PubMed
description BACKGROUND AND PURPOSE: Candida albicans is the most common Candida species (sp.) isolated from fungal infections. Azole resistance in Candida species has been considerably increased in the last decades. Given the toxicity of the antimicrobial drugs, resistance to antifungal agents, and drug interactions, the identification of new antifungal agents seems essential. In this study, we assessed the antifungal effects of biogenic selenium nanoparticles on C. albicans and determined the expression of ERG11 and CDR1 genes. MATERIALS AND METHODS: Selenium nanoparticles were synthesized with Bacillus sp. MSH-1. The ultrastructure of selenium nanoparticles was evaluated with a transmission electron microscope. The antifungal susceptibility test was performed according to the modified Clinical and Laboratory Standards Institute M27-A3 standard protocol. The expression levels of the CDR1 and ERG11 genes were analyzed using the quantitative real-time polymerase chain reaction (PCR) assay. RESULTS: The azole-resistant C. albicans and wild type C. albicans strains were inhibited by 100 and 70 µg/mL of selenium nanoparticle concentrations, respectively. The expression of CDR1 and ERG11 genes was significantly down-regulated in these selenium nanoparticle concentrations. CONCLUSION: As the findings indicated, selenium nanoparticles had an appropriate antifungal activity against fluconazole-resistant and -susceptible C. albicans strains. Accordingly, these nanoparticles reduced the expression of CDR1 and ERG11 genes associated with azole resistance. Further studies are needed to investigate the synergistic effects of selenium nanoparticles using other antifungal drugs.
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spelling pubmed-59149222018-04-27 Effect of biogenic selenium nanoparticles on ERG11 and CDR1 gene expression in both fluconazole-resistant and -susceptible Candida albicans isolates Parsameher, Nasrin Rezaei, Sassan Khodavasiy, Sadegh Salari, Samira Hadizade, Sanaz Kord, Mohammad Ayatollahi Mousavi, Seyed Amin Curr Med Mycol Original Article BACKGROUND AND PURPOSE: Candida albicans is the most common Candida species (sp.) isolated from fungal infections. Azole resistance in Candida species has been considerably increased in the last decades. Given the toxicity of the antimicrobial drugs, resistance to antifungal agents, and drug interactions, the identification of new antifungal agents seems essential. In this study, we assessed the antifungal effects of biogenic selenium nanoparticles on C. albicans and determined the expression of ERG11 and CDR1 genes. MATERIALS AND METHODS: Selenium nanoparticles were synthesized with Bacillus sp. MSH-1. The ultrastructure of selenium nanoparticles was evaluated with a transmission electron microscope. The antifungal susceptibility test was performed according to the modified Clinical and Laboratory Standards Institute M27-A3 standard protocol. The expression levels of the CDR1 and ERG11 genes were analyzed using the quantitative real-time polymerase chain reaction (PCR) assay. RESULTS: The azole-resistant C. albicans and wild type C. albicans strains were inhibited by 100 and 70 µg/mL of selenium nanoparticle concentrations, respectively. The expression of CDR1 and ERG11 genes was significantly down-regulated in these selenium nanoparticle concentrations. CONCLUSION: As the findings indicated, selenium nanoparticles had an appropriate antifungal activity against fluconazole-resistant and -susceptible C. albicans strains. Accordingly, these nanoparticles reduced the expression of CDR1 and ERG11 genes associated with azole resistance. Further studies are needed to investigate the synergistic effects of selenium nanoparticles using other antifungal drugs. Iranian Society of Medical Mycology 2017-09 /pmc/articles/PMC5914922/ /pubmed/29707669 http://dx.doi.org/10.29252/cmm.3.3.16 Text en © 2017, Published by Mazandaran University of Medical Sciences on behalf of Iranian Society of Medical Mycology and Invasive Fungi Research Center. This is an Open Access article distributed under the terms of the Creative Commons Attribution License, (http://creativecommons.org/licenses/by/4.0/) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Article
Parsameher, Nasrin
Rezaei, Sassan
Khodavasiy, Sadegh
Salari, Samira
Hadizade, Sanaz
Kord, Mohammad
Ayatollahi Mousavi, Seyed Amin
Effect of biogenic selenium nanoparticles on ERG11 and CDR1 gene expression in both fluconazole-resistant and -susceptible Candida albicans isolates
title Effect of biogenic selenium nanoparticles on ERG11 and CDR1 gene expression in both fluconazole-resistant and -susceptible Candida albicans isolates
title_full Effect of biogenic selenium nanoparticles on ERG11 and CDR1 gene expression in both fluconazole-resistant and -susceptible Candida albicans isolates
title_fullStr Effect of biogenic selenium nanoparticles on ERG11 and CDR1 gene expression in both fluconazole-resistant and -susceptible Candida albicans isolates
title_full_unstemmed Effect of biogenic selenium nanoparticles on ERG11 and CDR1 gene expression in both fluconazole-resistant and -susceptible Candida albicans isolates
title_short Effect of biogenic selenium nanoparticles on ERG11 and CDR1 gene expression in both fluconazole-resistant and -susceptible Candida albicans isolates
title_sort effect of biogenic selenium nanoparticles on erg11 and cdr1 gene expression in both fluconazole-resistant and -susceptible candida albicans isolates
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5914922/
https://www.ncbi.nlm.nih.gov/pubmed/29707669
http://dx.doi.org/10.29252/cmm.3.3.16
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