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Neurotropism and behavioral changes associated with Zika infection in the vector Aedes aegypti
Understanding Zika virus infection dynamics is essential, as its recent emergence revealed possible devastating neuropathologies in humans, thus causing a major threat to public health worldwide. Recent research allowed breakthrough in our understanding of the virus and host pathogenesis; however, l...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5915379/ https://www.ncbi.nlm.nih.gov/pubmed/29691362 http://dx.doi.org/10.1038/s41426-018-0069-2 |
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author | Gaburro, Julie Bhatti, Asim Harper, Jenni Jeanne, Isabelle Dearnley, Megan Green, Diane Nahavandi, Saeid Paradkar, Prasad N. Duchemin, Jean-Bernard |
author_facet | Gaburro, Julie Bhatti, Asim Harper, Jenni Jeanne, Isabelle Dearnley, Megan Green, Diane Nahavandi, Saeid Paradkar, Prasad N. Duchemin, Jean-Bernard |
author_sort | Gaburro, Julie |
collection | PubMed |
description | Understanding Zika virus infection dynamics is essential, as its recent emergence revealed possible devastating neuropathologies in humans, thus causing a major threat to public health worldwide. Recent research allowed breakthrough in our understanding of the virus and host pathogenesis; however, little is known on its impact on its main vector, Aedes aegypti. Here we show how Zika virus targets Aedes aegypti’s neurons and induces changes in its behavior. Results are compared to dengue virus, another flavivirus, which triggers a different pattern of behavioral changes. We used microelectrode array technology to record electrical spiking activity of mosquito primary neurons post infections and discovered that only Zika virus causes an increase in spiking activity of the neuronal network. Confocal microscopy also revealed an increase in synapse connections for Zika virus-infected neuronal networks. Interestingly, the results also showed that mosquito responds to infection by overexpressing glutamate regulatory genes while maintaining virus levels. This neuro-excitation, possibly via glutamate, could contribute to the observed behavioral changes in Zika virus-infected Aedes aegypti females. This study reveals the importance of virus-vector interaction in arbovirus neurotropism, in humans and vector. However, it appears that the consequences differ in the two hosts, with neuropathology in human host, while behavioral changes in the mosquito vector that may be advantageous to the virus. |
format | Online Article Text |
id | pubmed-5915379 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-59153792018-04-25 Neurotropism and behavioral changes associated with Zika infection in the vector Aedes aegypti Gaburro, Julie Bhatti, Asim Harper, Jenni Jeanne, Isabelle Dearnley, Megan Green, Diane Nahavandi, Saeid Paradkar, Prasad N. Duchemin, Jean-Bernard Emerg Microbes Infect Article Understanding Zika virus infection dynamics is essential, as its recent emergence revealed possible devastating neuropathologies in humans, thus causing a major threat to public health worldwide. Recent research allowed breakthrough in our understanding of the virus and host pathogenesis; however, little is known on its impact on its main vector, Aedes aegypti. Here we show how Zika virus targets Aedes aegypti’s neurons and induces changes in its behavior. Results are compared to dengue virus, another flavivirus, which triggers a different pattern of behavioral changes. We used microelectrode array technology to record electrical spiking activity of mosquito primary neurons post infections and discovered that only Zika virus causes an increase in spiking activity of the neuronal network. Confocal microscopy also revealed an increase in synapse connections for Zika virus-infected neuronal networks. Interestingly, the results also showed that mosquito responds to infection by overexpressing glutamate regulatory genes while maintaining virus levels. This neuro-excitation, possibly via glutamate, could contribute to the observed behavioral changes in Zika virus-infected Aedes aegypti females. This study reveals the importance of virus-vector interaction in arbovirus neurotropism, in humans and vector. However, it appears that the consequences differ in the two hosts, with neuropathology in human host, while behavioral changes in the mosquito vector that may be advantageous to the virus. Nature Publishing Group UK 2018-04-25 /pmc/articles/PMC5915379/ /pubmed/29691362 http://dx.doi.org/10.1038/s41426-018-0069-2 Text en © The Author(s) 2018 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Article Gaburro, Julie Bhatti, Asim Harper, Jenni Jeanne, Isabelle Dearnley, Megan Green, Diane Nahavandi, Saeid Paradkar, Prasad N. Duchemin, Jean-Bernard Neurotropism and behavioral changes associated with Zika infection in the vector Aedes aegypti |
title | Neurotropism and behavioral changes associated with Zika infection in the vector Aedes aegypti |
title_full | Neurotropism and behavioral changes associated with Zika infection in the vector Aedes aegypti |
title_fullStr | Neurotropism and behavioral changes associated with Zika infection in the vector Aedes aegypti |
title_full_unstemmed | Neurotropism and behavioral changes associated with Zika infection in the vector Aedes aegypti |
title_short | Neurotropism and behavioral changes associated with Zika infection in the vector Aedes aegypti |
title_sort | neurotropism and behavioral changes associated with zika infection in the vector aedes aegypti |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5915379/ https://www.ncbi.nlm.nih.gov/pubmed/29691362 http://dx.doi.org/10.1038/s41426-018-0069-2 |
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