Cargando…
A novel sheet-like virus particle array is a hallmark of Zika virus infection
Zika virus (ZIKV) is an emerging flavivirus that caused thousands of human infections in recent years. Compared to other human flaviviruses, ZIKV replication is not well understood. Using fluorescent, transmission electron, and focused ion beam-scanning electron microscopy, we examined ZIKV replicat...
Autores principales: | , , , , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2018
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5915449/ https://www.ncbi.nlm.nih.gov/pubmed/29691373 http://dx.doi.org/10.1038/s41426-018-0071-8 |
_version_ | 1783316862755405824 |
---|---|
author | Liu, Jun Kline, Brandon A. Kenny, Tara A. Smith, Darci R. Soloveva, Veronica Beitzel, Brett Pang, Song Lockett, Stephen Hess, Harald F. Palacios, Gustavo Kuhn, Jens H. Sun, Mei G. Zeng, Xiankun |
author_facet | Liu, Jun Kline, Brandon A. Kenny, Tara A. Smith, Darci R. Soloveva, Veronica Beitzel, Brett Pang, Song Lockett, Stephen Hess, Harald F. Palacios, Gustavo Kuhn, Jens H. Sun, Mei G. Zeng, Xiankun |
author_sort | Liu, Jun |
collection | PubMed |
description | Zika virus (ZIKV) is an emerging flavivirus that caused thousands of human infections in recent years. Compared to other human flaviviruses, ZIKV replication is not well understood. Using fluorescent, transmission electron, and focused ion beam-scanning electron microscopy, we examined ZIKV replication dynamics in Vero 76 cells and in the brains of infected laboratory mice. We observed the progressive development of a perinuclear flaviviral replication factory both in vitro and in vivo. In vitro, we illustrated the ZIKV lifecycle from particle cell entry to egress. ZIKV particles assembled and aggregated in an induced convoluted membrane structure and ZIKV strain-specific membranous vesicles. While most mature virus particles egressed via membrane budding, some particles also likely trafficked through late endosomes and egressed through membrane abscission. Interestingly, we consistently observed a novel sheet-like virus particle array consisting of a single layer of ZIKV particles. Our study further defines ZIKV replication and identifies a novel hallmark of ZIKV infection. |
format | Online Article Text |
id | pubmed-5915449 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-59154492018-04-25 A novel sheet-like virus particle array is a hallmark of Zika virus infection Liu, Jun Kline, Brandon A. Kenny, Tara A. Smith, Darci R. Soloveva, Veronica Beitzel, Brett Pang, Song Lockett, Stephen Hess, Harald F. Palacios, Gustavo Kuhn, Jens H. Sun, Mei G. Zeng, Xiankun Emerg Microbes Infect Article Zika virus (ZIKV) is an emerging flavivirus that caused thousands of human infections in recent years. Compared to other human flaviviruses, ZIKV replication is not well understood. Using fluorescent, transmission electron, and focused ion beam-scanning electron microscopy, we examined ZIKV replication dynamics in Vero 76 cells and in the brains of infected laboratory mice. We observed the progressive development of a perinuclear flaviviral replication factory both in vitro and in vivo. In vitro, we illustrated the ZIKV lifecycle from particle cell entry to egress. ZIKV particles assembled and aggregated in an induced convoluted membrane structure and ZIKV strain-specific membranous vesicles. While most mature virus particles egressed via membrane budding, some particles also likely trafficked through late endosomes and egressed through membrane abscission. Interestingly, we consistently observed a novel sheet-like virus particle array consisting of a single layer of ZIKV particles. Our study further defines ZIKV replication and identifies a novel hallmark of ZIKV infection. Nature Publishing Group UK 2018-04-25 /pmc/articles/PMC5915449/ /pubmed/29691373 http://dx.doi.org/10.1038/s41426-018-0071-8 Text en © The Author(s) 2018 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visithttp://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Article Liu, Jun Kline, Brandon A. Kenny, Tara A. Smith, Darci R. Soloveva, Veronica Beitzel, Brett Pang, Song Lockett, Stephen Hess, Harald F. Palacios, Gustavo Kuhn, Jens H. Sun, Mei G. Zeng, Xiankun A novel sheet-like virus particle array is a hallmark of Zika virus infection |
title | A novel sheet-like virus particle array is a hallmark of Zika virus infection |
title_full | A novel sheet-like virus particle array is a hallmark of Zika virus infection |
title_fullStr | A novel sheet-like virus particle array is a hallmark of Zika virus infection |
title_full_unstemmed | A novel sheet-like virus particle array is a hallmark of Zika virus infection |
title_short | A novel sheet-like virus particle array is a hallmark of Zika virus infection |
title_sort | novel sheet-like virus particle array is a hallmark of zika virus infection |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5915449/ https://www.ncbi.nlm.nih.gov/pubmed/29691373 http://dx.doi.org/10.1038/s41426-018-0071-8 |
work_keys_str_mv | AT liujun anovelsheetlikevirusparticlearrayisahallmarkofzikavirusinfection AT klinebrandona anovelsheetlikevirusparticlearrayisahallmarkofzikavirusinfection AT kennytaraa anovelsheetlikevirusparticlearrayisahallmarkofzikavirusinfection AT smithdarcir anovelsheetlikevirusparticlearrayisahallmarkofzikavirusinfection AT solovevaveronica anovelsheetlikevirusparticlearrayisahallmarkofzikavirusinfection AT beitzelbrett anovelsheetlikevirusparticlearrayisahallmarkofzikavirusinfection AT pangsong anovelsheetlikevirusparticlearrayisahallmarkofzikavirusinfection AT lockettstephen anovelsheetlikevirusparticlearrayisahallmarkofzikavirusinfection AT hessharaldf anovelsheetlikevirusparticlearrayisahallmarkofzikavirusinfection AT palaciosgustavo anovelsheetlikevirusparticlearrayisahallmarkofzikavirusinfection AT kuhnjensh anovelsheetlikevirusparticlearrayisahallmarkofzikavirusinfection AT sunmeig anovelsheetlikevirusparticlearrayisahallmarkofzikavirusinfection AT zengxiankun anovelsheetlikevirusparticlearrayisahallmarkofzikavirusinfection AT liujun novelsheetlikevirusparticlearrayisahallmarkofzikavirusinfection AT klinebrandona novelsheetlikevirusparticlearrayisahallmarkofzikavirusinfection AT kennytaraa novelsheetlikevirusparticlearrayisahallmarkofzikavirusinfection AT smithdarcir novelsheetlikevirusparticlearrayisahallmarkofzikavirusinfection AT solovevaveronica novelsheetlikevirusparticlearrayisahallmarkofzikavirusinfection AT beitzelbrett novelsheetlikevirusparticlearrayisahallmarkofzikavirusinfection AT pangsong novelsheetlikevirusparticlearrayisahallmarkofzikavirusinfection AT lockettstephen novelsheetlikevirusparticlearrayisahallmarkofzikavirusinfection AT hessharaldf novelsheetlikevirusparticlearrayisahallmarkofzikavirusinfection AT palaciosgustavo novelsheetlikevirusparticlearrayisahallmarkofzikavirusinfection AT kuhnjensh novelsheetlikevirusparticlearrayisahallmarkofzikavirusinfection AT sunmeig novelsheetlikevirusparticlearrayisahallmarkofzikavirusinfection AT zengxiankun novelsheetlikevirusparticlearrayisahallmarkofzikavirusinfection |