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RNA-Seq analysis of interferon inducible p204-mediated network in anti-tumor immunity

p204, a murine member of the interferon-inducible p200 protein family, and its human analogue, IFI16, have been shown to function as tumor suppressors in vitro, but the molecular events involved, in particular in vivo, remain unclear. Herein we induced the Lewis Lung carcinoma (LLC) murine model of...

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Autores principales: Jian, Jinlong, Wei, Wei, Yin, Guowei, Hettinghouse, Aubryanna, Liu, Chuanju, Shi, Yongxiang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5915582/
https://www.ncbi.nlm.nih.gov/pubmed/29691417
http://dx.doi.org/10.1038/s41598-018-24561-2
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author Jian, Jinlong
Wei, Wei
Yin, Guowei
Hettinghouse, Aubryanna
Liu, Chuanju
Shi, Yongxiang
author_facet Jian, Jinlong
Wei, Wei
Yin, Guowei
Hettinghouse, Aubryanna
Liu, Chuanju
Shi, Yongxiang
author_sort Jian, Jinlong
collection PubMed
description p204, a murine member of the interferon-inducible p200 protein family, and its human analogue, IFI16, have been shown to function as tumor suppressors in vitro, but the molecular events involved, in particular in vivo, remain unclear. Herein we induced the Lewis Lung carcinoma (LLC) murine model of human lung cancer in p204 null mice (KO) and their control littermates (WT). We compared the transcriptome in spleen from WT and p204 KO mice using a high-throughput RNA-sequencing array. A total 30.02 Gb of clean data were obtained, and overall Q30% was greater than 90.54%. More than 75% of clean data from 12 transcriptome samples were mapped to exons. The results showed that only 11 genes exhibited altered expression in untreated p204 KO mice relative to untreated WT mice, while 393 altered genes were identified in tumor-bearing p204 KO mice when compared with tumor-bearing WT mice. Further differentially expressed gene cluster and gene ontology consortium classification revealed that numerous cytokines and their receptors, chemoattractant molecules, and adhesion molecules were significantly induced in p204 KO mice. This study provides novel insights to the p204 network in anti-tumor immune response and also presents a foundation for future work concerning p204-mediated gene expressions and pathways.
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spelling pubmed-59155822018-04-30 RNA-Seq analysis of interferon inducible p204-mediated network in anti-tumor immunity Jian, Jinlong Wei, Wei Yin, Guowei Hettinghouse, Aubryanna Liu, Chuanju Shi, Yongxiang Sci Rep Article p204, a murine member of the interferon-inducible p200 protein family, and its human analogue, IFI16, have been shown to function as tumor suppressors in vitro, but the molecular events involved, in particular in vivo, remain unclear. Herein we induced the Lewis Lung carcinoma (LLC) murine model of human lung cancer in p204 null mice (KO) and their control littermates (WT). We compared the transcriptome in spleen from WT and p204 KO mice using a high-throughput RNA-sequencing array. A total 30.02 Gb of clean data were obtained, and overall Q30% was greater than 90.54%. More than 75% of clean data from 12 transcriptome samples were mapped to exons. The results showed that only 11 genes exhibited altered expression in untreated p204 KO mice relative to untreated WT mice, while 393 altered genes were identified in tumor-bearing p204 KO mice when compared with tumor-bearing WT mice. Further differentially expressed gene cluster and gene ontology consortium classification revealed that numerous cytokines and their receptors, chemoattractant molecules, and adhesion molecules were significantly induced in p204 KO mice. This study provides novel insights to the p204 network in anti-tumor immune response and also presents a foundation for future work concerning p204-mediated gene expressions and pathways. Nature Publishing Group UK 2018-04-24 /pmc/articles/PMC5915582/ /pubmed/29691417 http://dx.doi.org/10.1038/s41598-018-24561-2 Text en © The Author(s) 2018 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Jian, Jinlong
Wei, Wei
Yin, Guowei
Hettinghouse, Aubryanna
Liu, Chuanju
Shi, Yongxiang
RNA-Seq analysis of interferon inducible p204-mediated network in anti-tumor immunity
title RNA-Seq analysis of interferon inducible p204-mediated network in anti-tumor immunity
title_full RNA-Seq analysis of interferon inducible p204-mediated network in anti-tumor immunity
title_fullStr RNA-Seq analysis of interferon inducible p204-mediated network in anti-tumor immunity
title_full_unstemmed RNA-Seq analysis of interferon inducible p204-mediated network in anti-tumor immunity
title_short RNA-Seq analysis of interferon inducible p204-mediated network in anti-tumor immunity
title_sort rna-seq analysis of interferon inducible p204-mediated network in anti-tumor immunity
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5915582/
https://www.ncbi.nlm.nih.gov/pubmed/29691417
http://dx.doi.org/10.1038/s41598-018-24561-2
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