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The role of PRP and adipose tissue-derived keratinocytes on burn wound healing in diabetic rats

[Image: see text] Introduction: Diabetic burn wounds and ulcers are significant complications of diabetic patients. The aim of this study is to investigate the use of platelet rich-plasma (PRP) and/or keratinocyte-like cells (KLCs) in diabetic thermal wound rat model and to evaluate EGF, FGF-2, TGF-...

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Autores principales: Hosseini Mansoub, Navid, Gürdal, Mehmet, Karadadaş, Elif, Kabadayi, Hilal, Vatansever, Seda, Ercan, Gulinnaz
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Tabriz University of Medical Sciences 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5915708/
https://www.ncbi.nlm.nih.gov/pubmed/29713597
http://dx.doi.org/10.15171/bi.2018.02
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author Hosseini Mansoub, Navid
Gürdal, Mehmet
Karadadaş, Elif
Kabadayi, Hilal
Vatansever, Seda
Ercan, Gulinnaz
author_facet Hosseini Mansoub, Navid
Gürdal, Mehmet
Karadadaş, Elif
Kabadayi, Hilal
Vatansever, Seda
Ercan, Gulinnaz
author_sort Hosseini Mansoub, Navid
collection PubMed
description [Image: see text] Introduction: Diabetic burn wounds and ulcers are significant complications of diabetic patients. The aim of this study is to investigate the use of platelet rich-plasma (PRP) and/or keratinocyte-like cells (KLCs) in diabetic thermal wound rat model and to evaluate EGF, FGF-2, TGF-β1, COL1α2, MCP-1 and VEGF-α as wound healing markers at gene expression level. Method: In this study, we used adipose tissue as the source of mesenchymal stem cells (MSCs) and differentiated MSCs into KLCs. KLCs were characterized and transferred to the burn areas on the dorsum of streptozotocine (STZ)-induced diabetic rats. We prepared PRP from rat blood and evaluated its effect alone or in combination with KLCs. On 3(rd), 7(th), 10(th) and 14(th) days after treatment, wound areas were measured and biopsy samples were excised from the wound areas of the KLCs and/or PRP-treated and untreated diabetic rats to analyze gene expression levels of wound healing markers by qPCR. Results: We observed that, wound contraction started earlier in the PRP and/or KLCs-treated groups in comparison to the control group. However, PRP and KLCs when applied in combination showed additive affect in wound healing. In all groups treated with KLCs and/or PRP, the gene expression levels of evaluated growth factors and COL1α2 increased, while MCP-1 levels decreased when compared to the untreated diabetic rats. In addition, the most prominent difference in qPCR results belongs to combined PRP and KLCs-treated group. Conclusion: We demonstrated that applying PRP and KLCs in combination has a greater potential for treatment of diabetic burn wounds.
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spelling pubmed-59157082018-04-30 The role of PRP and adipose tissue-derived keratinocytes on burn wound healing in diabetic rats Hosseini Mansoub, Navid Gürdal, Mehmet Karadadaş, Elif Kabadayi, Hilal Vatansever, Seda Ercan, Gulinnaz Bioimpacts Original Research [Image: see text] Introduction: Diabetic burn wounds and ulcers are significant complications of diabetic patients. The aim of this study is to investigate the use of platelet rich-plasma (PRP) and/or keratinocyte-like cells (KLCs) in diabetic thermal wound rat model and to evaluate EGF, FGF-2, TGF-β1, COL1α2, MCP-1 and VEGF-α as wound healing markers at gene expression level. Method: In this study, we used adipose tissue as the source of mesenchymal stem cells (MSCs) and differentiated MSCs into KLCs. KLCs were characterized and transferred to the burn areas on the dorsum of streptozotocine (STZ)-induced diabetic rats. We prepared PRP from rat blood and evaluated its effect alone or in combination with KLCs. On 3(rd), 7(th), 10(th) and 14(th) days after treatment, wound areas were measured and biopsy samples were excised from the wound areas of the KLCs and/or PRP-treated and untreated diabetic rats to analyze gene expression levels of wound healing markers by qPCR. Results: We observed that, wound contraction started earlier in the PRP and/or KLCs-treated groups in comparison to the control group. However, PRP and KLCs when applied in combination showed additive affect in wound healing. In all groups treated with KLCs and/or PRP, the gene expression levels of evaluated growth factors and COL1α2 increased, while MCP-1 levels decreased when compared to the untreated diabetic rats. In addition, the most prominent difference in qPCR results belongs to combined PRP and KLCs-treated group. Conclusion: We demonstrated that applying PRP and KLCs in combination has a greater potential for treatment of diabetic burn wounds. Tabriz University of Medical Sciences 2018 2017-11-18 /pmc/articles/PMC5915708/ /pubmed/29713597 http://dx.doi.org/10.15171/bi.2018.02 Text en © 2018 The Author(s) This work is published by BioImpacts as an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by-nc/4.0/). Non-commercial uses of the work are permitted, provided the original work is properly cited.
spellingShingle Original Research
Hosseini Mansoub, Navid
Gürdal, Mehmet
Karadadaş, Elif
Kabadayi, Hilal
Vatansever, Seda
Ercan, Gulinnaz
The role of PRP and adipose tissue-derived keratinocytes on burn wound healing in diabetic rats
title The role of PRP and adipose tissue-derived keratinocytes on burn wound healing in diabetic rats
title_full The role of PRP and adipose tissue-derived keratinocytes on burn wound healing in diabetic rats
title_fullStr The role of PRP and adipose tissue-derived keratinocytes on burn wound healing in diabetic rats
title_full_unstemmed The role of PRP and adipose tissue-derived keratinocytes on burn wound healing in diabetic rats
title_short The role of PRP and adipose tissue-derived keratinocytes on burn wound healing in diabetic rats
title_sort role of prp and adipose tissue-derived keratinocytes on burn wound healing in diabetic rats
topic Original Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5915708/
https://www.ncbi.nlm.nih.gov/pubmed/29713597
http://dx.doi.org/10.15171/bi.2018.02
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