Targeting Long Noncoding RNA HMMR-AS1 Suppresses and Radiosensitizes Glioblastoma

Emergent evidences revealed that long noncoding RNAs (lncRNAs) participate in neoplastic progression. HMMR is an oncogene that is highly expressed in glioblastoma (GBM) and supports GBM growth. Whether lncRNAs regulate HMMR in GBM remains unknown. Herein, we identify that an HMMR antisense lncRNA, H...

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Detalles Bibliográficos
Autores principales: Li, Junyang, Ji, Xiangjun, Wang, Handong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Neoplasia Press 2018
Materias:
Original article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5915996/
https://www.ncbi.nlm.nih.gov/pubmed/29574252
http://dx.doi.org/10.1016/j.neo.2018.02.010
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author Li, Junyang
Ji, Xiangjun
Wang, Handong
author_facet Li, Junyang
Ji, Xiangjun
Wang, Handong
author_sort Li, Junyang
collection PubMed
description Emergent evidences revealed that long noncoding RNAs (lncRNAs) participate in neoplastic progression. HMMR is an oncogene that is highly expressed in glioblastoma (GBM) and supports GBM growth. Whether lncRNAs regulate HMMR in GBM remains unknown. Herein, we identify that an HMMR antisense lncRNA, HMMR-AS1, is hyperexpressed in GBM cell lines and stabilizes HMMR mRNA. Knockdown of HMMR-AS1 reduces HMMR expression; inhibits cell migration, invasion, and mesenchymal phenotypes; and suppresses GBM cell growth both in vitro and in vivo. Moreover, knockdown of HMMR-AS1 radiosensitizes GBM by reducing DNA repair proteins ATM, RAD51, and BMI1. Our data demonstrate a mechanism of sense-antisense interference between HMMR and HMMR-AS1 in GBM and suggest that targeting HMMR-AS1 is a potential strategy for GBM treatment.
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spelling pubmed-59159962018-04-27 Targeting Long Noncoding RNA HMMR-AS1 Suppresses and Radiosensitizes Glioblastoma Li, Junyang Ji, Xiangjun Wang, Handong Neoplasia Original article Emergent evidences revealed that long noncoding RNAs (lncRNAs) participate in neoplastic progression. HMMR is an oncogene that is highly expressed in glioblastoma (GBM) and supports GBM growth. Whether lncRNAs regulate HMMR in GBM remains unknown. Herein, we identify that an HMMR antisense lncRNA, HMMR-AS1, is hyperexpressed in GBM cell lines and stabilizes HMMR mRNA. Knockdown of HMMR-AS1 reduces HMMR expression; inhibits cell migration, invasion, and mesenchymal phenotypes; and suppresses GBM cell growth both in vitro and in vivo. Moreover, knockdown of HMMR-AS1 radiosensitizes GBM by reducing DNA repair proteins ATM, RAD51, and BMI1. Our data demonstrate a mechanism of sense-antisense interference between HMMR and HMMR-AS1 in GBM and suggest that targeting HMMR-AS1 is a potential strategy for GBM treatment. Neoplasia Press 2018-03-22 /pmc/articles/PMC5915996/ /pubmed/29574252 http://dx.doi.org/10.1016/j.neo.2018.02.010 Text en © 2017 Published by Elsevier Inc. on behalf of SOCIETY. . http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Original article
Li, Junyang
Ji, Xiangjun
Wang, Handong
Targeting Long Noncoding RNA HMMR-AS1 Suppresses and Radiosensitizes Glioblastoma
title Targeting Long Noncoding RNA HMMR-AS1 Suppresses and Radiosensitizes Glioblastoma
title_full Targeting Long Noncoding RNA HMMR-AS1 Suppresses and Radiosensitizes Glioblastoma
title_fullStr Targeting Long Noncoding RNA HMMR-AS1 Suppresses and Radiosensitizes Glioblastoma
title_full_unstemmed Targeting Long Noncoding RNA HMMR-AS1 Suppresses and Radiosensitizes Glioblastoma
title_short Targeting Long Noncoding RNA HMMR-AS1 Suppresses and Radiosensitizes Glioblastoma
title_sort targeting long noncoding rna hmmr-as1 suppresses and radiosensitizes glioblastoma
topic Original article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5915996/
https://www.ncbi.nlm.nih.gov/pubmed/29574252
http://dx.doi.org/10.1016/j.neo.2018.02.010
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