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Effect of Sustained Hypoxia on Autophagy of Genioglossus Muscle-Derived Stem Cells
BACKGROUND: Previous studies have demonstrated that sustained hypoxia in people with obstructive sleep apnea (OSA) impairs upper airway muscle activity, but the underlying mechanism remains poorly understood. As autophagy acts as an important regulator under hypoxia stress, we performed an in vitro...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
International Scientific Literature, Inc.
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5916094/ https://www.ncbi.nlm.nih.gov/pubmed/29652869 http://dx.doi.org/10.12659/MSM.906195 |
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author | Zhang, Dongsheng Jia, Shanshan Wang, Hengkun Huang, Shengyun Xiao, Lili Ma, Li Liu, Guangping Gong, Kun Xu, Le |
author_facet | Zhang, Dongsheng Jia, Shanshan Wang, Hengkun Huang, Shengyun Xiao, Lili Ma, Li Liu, Guangping Gong, Kun Xu, Le |
author_sort | Zhang, Dongsheng |
collection | PubMed |
description | BACKGROUND: Previous studies have demonstrated that sustained hypoxia in people with obstructive sleep apnea (OSA) impairs upper airway muscle activity, but the underlying mechanism remains poorly understood. As autophagy acts as an important regulator under hypoxia stress, we performed an in vitro investigation of the effects of sustained hypoxia on autophagy of genioglossus muscle-derived stem cells (GG MDSC), an important component of the upper airway muscle. MATERIAL/METHODS: Genioglossus MDSCs were obtained from Sprague-Dawley (SD) rats and identified by using immunofluorescence staining for CD34, Sca-1, and desmin. GG MDSCs were incubated under normoxic or sustained hypoxic conditions for different periods of time. Western blotting was used to detect LC3 and Beclin 1, which are 2 important proteins in autophagy flux, and autophagolysosomes accumulation was observed by transmission electron microscopy (TEM). The mRNA and protein levels of HIF-1α and BNIP3 were evaluated by RT-PCR and Western blot analysis, respectively. RESULTS: Our study shows that sustained hypoxia promotes the expression of LC3BII and Beclin 1 in GG MDSCs in a time-dependent manner. TEM showed an increased number of autophagolysosomes in GG MDSCs under sustained hypoxia for 12 and 24 h. In addition, hypoxia activated the HIF-1α/BNIP3 signal pathway both at protein levels (shown by Western blot) and at mRNA levels (shown by RT-PCR). CONCLUSIONS: Our study shows that sustained hypoxia promotes autophagy in GG MDSCs, and the HIF-1α/BNIP3 signal pathway was involved in this process. |
format | Online Article Text |
id | pubmed-5916094 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | International Scientific Literature, Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-59160942018-04-27 Effect of Sustained Hypoxia on Autophagy of Genioglossus Muscle-Derived Stem Cells Zhang, Dongsheng Jia, Shanshan Wang, Hengkun Huang, Shengyun Xiao, Lili Ma, Li Liu, Guangping Gong, Kun Xu, Le Med Sci Monit Animal Study BACKGROUND: Previous studies have demonstrated that sustained hypoxia in people with obstructive sleep apnea (OSA) impairs upper airway muscle activity, but the underlying mechanism remains poorly understood. As autophagy acts as an important regulator under hypoxia stress, we performed an in vitro investigation of the effects of sustained hypoxia on autophagy of genioglossus muscle-derived stem cells (GG MDSC), an important component of the upper airway muscle. MATERIAL/METHODS: Genioglossus MDSCs were obtained from Sprague-Dawley (SD) rats and identified by using immunofluorescence staining for CD34, Sca-1, and desmin. GG MDSCs were incubated under normoxic or sustained hypoxic conditions for different periods of time. Western blotting was used to detect LC3 and Beclin 1, which are 2 important proteins in autophagy flux, and autophagolysosomes accumulation was observed by transmission electron microscopy (TEM). The mRNA and protein levels of HIF-1α and BNIP3 were evaluated by RT-PCR and Western blot analysis, respectively. RESULTS: Our study shows that sustained hypoxia promotes the expression of LC3BII and Beclin 1 in GG MDSCs in a time-dependent manner. TEM showed an increased number of autophagolysosomes in GG MDSCs under sustained hypoxia for 12 and 24 h. In addition, hypoxia activated the HIF-1α/BNIP3 signal pathway both at protein levels (shown by Western blot) and at mRNA levels (shown by RT-PCR). CONCLUSIONS: Our study shows that sustained hypoxia promotes autophagy in GG MDSCs, and the HIF-1α/BNIP3 signal pathway was involved in this process. International Scientific Literature, Inc. 2018-04-13 /pmc/articles/PMC5916094/ /pubmed/29652869 http://dx.doi.org/10.12659/MSM.906195 Text en © Med Sci Monit, 2018 This work is licensed under Creative Common Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0 (https://creativecommons.org/licenses/by-nc-nd/4.0/) ) |
spellingShingle | Animal Study Zhang, Dongsheng Jia, Shanshan Wang, Hengkun Huang, Shengyun Xiao, Lili Ma, Li Liu, Guangping Gong, Kun Xu, Le Effect of Sustained Hypoxia on Autophagy of Genioglossus Muscle-Derived Stem Cells |
title | Effect of Sustained Hypoxia on Autophagy of Genioglossus Muscle-Derived Stem Cells |
title_full | Effect of Sustained Hypoxia on Autophagy of Genioglossus Muscle-Derived Stem Cells |
title_fullStr | Effect of Sustained Hypoxia on Autophagy of Genioglossus Muscle-Derived Stem Cells |
title_full_unstemmed | Effect of Sustained Hypoxia on Autophagy of Genioglossus Muscle-Derived Stem Cells |
title_short | Effect of Sustained Hypoxia on Autophagy of Genioglossus Muscle-Derived Stem Cells |
title_sort | effect of sustained hypoxia on autophagy of genioglossus muscle-derived stem cells |
topic | Animal Study |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5916094/ https://www.ncbi.nlm.nih.gov/pubmed/29652869 http://dx.doi.org/10.12659/MSM.906195 |
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