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In vitro synthesis of gene-length single-stranded DNA
Single-stranded DNA (ssDNA) increases the likelihood of homology directed repair with reduced cellular toxicity. However, ssDNA synthesis strategies are limited by the maximum length attainable, ranging from a few hundred nucleotides for chemical synthesis to a few thousand nucleotides for enzymatic...
Autores principales: | Veneziano, Rémi, Shepherd, Tyson R., Ratanalert, Sakul, Bellou, Leila, Tao, Chaoqun, Bathe, Mark |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5916881/ https://www.ncbi.nlm.nih.gov/pubmed/29695837 http://dx.doi.org/10.1038/s41598-018-24677-5 |
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