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The use of premature chromosome condensation to study in interphase cells the influence of environmental factors on human genetic material
Nowadays, there is a constantly increasing concern regarding the mutagenic and carcinogenic potential of a variety of harmful environmental factors to which humans are exposed in their natural and anthropogenic environment. These factors exert their hazardous potential in humans' personal (diet...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
TheScientificWorldJOURNAL
2006
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5917154/ https://www.ncbi.nlm.nih.gov/pubmed/17013523 http://dx.doi.org/10.1100/tsw.2006.210 |
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author | Hatzi, Vasiliki I. Terzoudi, Georgia I. Paraskevopoulou, Christina Makropoulos, Vasilios Matthopoulos, Demetrios P. Pantelias, Gabriel E. |
author_facet | Hatzi, Vasiliki I. Terzoudi, Georgia I. Paraskevopoulou, Christina Makropoulos, Vasilios Matthopoulos, Demetrios P. Pantelias, Gabriel E. |
author_sort | Hatzi, Vasiliki I. |
collection | PubMed |
description | Nowadays, there is a constantly increasing concern regarding the mutagenic and carcinogenic potential of a variety of harmful environmental factors to which humans are exposed in their natural and anthropogenic environment. These factors exert their hazardous potential in humans' personal (diet, smoking, pharmaceuticals, cosmetics) and occupational environment that constitute part of the anthropogenic environment. It is well known that genetic damage due to these factors has dramatic implications for human health. Since most of the environmental genotoxic factors induce arrest or delay in cell cycle progression, the conventional analysis of chromosomes at metaphase may underestimate their genotoxic potential. Premature Chromosome Condensation (PCC) induced either by means of cell fusion or specific chemicals, enables the microscopic visualization of interphase chromosomes whose morphology depends on the cell cycle stage, as well as the analysis of structural and numerical aberrations at the G1 and G2 phases of the cell cycle. The PCC has been successfully used in problems involving cell cycle analysis, diagnosis and prognosis of human leukaemia, assessment of interphase chromosome malformations resulting from exposure to radiation or chemicals, as well as elucidation of the mechanisms underlying the conversion of DNA damage into chromosomal damage. In this report, particular emphasis is given to the advantages of the PCC methodology used as an alternative to conventional metaphase analysis in answering questions in the fields of radiobiology, biological dosimetry, toxicogenetics, clinical cytogenetics and experimental therapeutics. |
format | Online Article Text |
id | pubmed-5917154 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2006 |
publisher | TheScientificWorldJOURNAL |
record_format | MEDLINE/PubMed |
spelling | pubmed-59171542018-06-03 The use of premature chromosome condensation to study in interphase cells the influence of environmental factors on human genetic material Hatzi, Vasiliki I. Terzoudi, Georgia I. Paraskevopoulou, Christina Makropoulos, Vasilios Matthopoulos, Demetrios P. Pantelias, Gabriel E. ScientificWorldJournal Review Article Nowadays, there is a constantly increasing concern regarding the mutagenic and carcinogenic potential of a variety of harmful environmental factors to which humans are exposed in their natural and anthropogenic environment. These factors exert their hazardous potential in humans' personal (diet, smoking, pharmaceuticals, cosmetics) and occupational environment that constitute part of the anthropogenic environment. It is well known that genetic damage due to these factors has dramatic implications for human health. Since most of the environmental genotoxic factors induce arrest or delay in cell cycle progression, the conventional analysis of chromosomes at metaphase may underestimate their genotoxic potential. Premature Chromosome Condensation (PCC) induced either by means of cell fusion or specific chemicals, enables the microscopic visualization of interphase chromosomes whose morphology depends on the cell cycle stage, as well as the analysis of structural and numerical aberrations at the G1 and G2 phases of the cell cycle. The PCC has been successfully used in problems involving cell cycle analysis, diagnosis and prognosis of human leukaemia, assessment of interphase chromosome malformations resulting from exposure to radiation or chemicals, as well as elucidation of the mechanisms underlying the conversion of DNA damage into chromosomal damage. In this report, particular emphasis is given to the advantages of the PCC methodology used as an alternative to conventional metaphase analysis in answering questions in the fields of radiobiology, biological dosimetry, toxicogenetics, clinical cytogenetics and experimental therapeutics. TheScientificWorldJOURNAL 2006-09-25 /pmc/articles/PMC5917154/ /pubmed/17013523 http://dx.doi.org/10.1100/tsw.2006.210 Text en Copyright © 2006 Vasiliki I. Hatzi et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Review Article Hatzi, Vasiliki I. Terzoudi, Georgia I. Paraskevopoulou, Christina Makropoulos, Vasilios Matthopoulos, Demetrios P. Pantelias, Gabriel E. The use of premature chromosome condensation to study in interphase cells the influence of environmental factors on human genetic material |
title | The use of premature chromosome condensation to study in interphase cells the influence of environmental factors on human genetic material |
title_full | The use of premature chromosome condensation to study in interphase cells the influence of environmental factors on human genetic material |
title_fullStr | The use of premature chromosome condensation to study in interphase cells the influence of environmental factors on human genetic material |
title_full_unstemmed | The use of premature chromosome condensation to study in interphase cells the influence of environmental factors on human genetic material |
title_short | The use of premature chromosome condensation to study in interphase cells the influence of environmental factors on human genetic material |
title_sort | use of premature chromosome condensation to study in interphase cells the influence of environmental factors on human genetic material |
topic | Review Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5917154/ https://www.ncbi.nlm.nih.gov/pubmed/17013523 http://dx.doi.org/10.1100/tsw.2006.210 |
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