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Electron Microscopic Radioautographic Study on Protein Synthesis in Hepatocyte Mitochondria of Aging Mice

For the purpose of studying the aging changes of intramitochondrial protein synthesis in mouse hepatocytes, 10 groups of aging mice, each consisting of 3 individuals (total 30), from fetal day 19 to postnatal month 24, were injected during development with 3H-leucine, a protein precursor, sacrificed...

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Autor principal: Nagata, Tetsuji
Formato: Online Artículo Texto
Lenguaje:English
Publicado: TheScientificWorldJOURNAL 2006
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5917294/
https://www.ncbi.nlm.nih.gov/pubmed/17173177
http://dx.doi.org/10.1100/tsw.2006.265
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author Nagata, Tetsuji
author_facet Nagata, Tetsuji
author_sort Nagata, Tetsuji
collection PubMed
description For the purpose of studying the aging changes of intramitochondrial protein synthesis in mouse hepatocytes, 10 groups of aging mice, each consisting of 3 individuals (total 30), from fetal day 19 to postnatal month 24, were injected during development with 3H-leucine, a protein precursor, sacrificed 1 h later, and the liver tissues processed for electron microscopic (EM) radioautography. On EM radioautograms obtained from each animal, the number of mitochondria, the number of labeled mitochondria, and the mitochondrial labeling index labeled with silver grains due to H-leucine showing protein synthesis in each mononucleate hepatocytes were counted and the averages in respective aging groups were compared. From the results, it was demonstrated that the numbers of mitochondria, the numbers of labeled mitochondria, and the labeling indices of intramitochondrial protein syntheses in mononucleate hepatocytes of mice at various ages from embryonic day 19 to postnatal month 24 increased and decreased due to development and aging of animals.
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spelling pubmed-59172942018-06-03 Electron Microscopic Radioautographic Study on Protein Synthesis in Hepatocyte Mitochondria of Aging Mice Nagata, Tetsuji ScientificWorldJournal Review Article For the purpose of studying the aging changes of intramitochondrial protein synthesis in mouse hepatocytes, 10 groups of aging mice, each consisting of 3 individuals (total 30), from fetal day 19 to postnatal month 24, were injected during development with 3H-leucine, a protein precursor, sacrificed 1 h later, and the liver tissues processed for electron microscopic (EM) radioautography. On EM radioautograms obtained from each animal, the number of mitochondria, the number of labeled mitochondria, and the mitochondrial labeling index labeled with silver grains due to H-leucine showing protein synthesis in each mononucleate hepatocytes were counted and the averages in respective aging groups were compared. From the results, it was demonstrated that the numbers of mitochondria, the numbers of labeled mitochondria, and the labeling indices of intramitochondrial protein syntheses in mononucleate hepatocytes of mice at various ages from embryonic day 19 to postnatal month 24 increased and decreased due to development and aging of animals. TheScientificWorldJOURNAL 2006-12-15 /pmc/articles/PMC5917294/ /pubmed/17173177 http://dx.doi.org/10.1100/tsw.2006.265 Text en Copyright © 2006 Tetsuji Nagata. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Review Article
Nagata, Tetsuji
Electron Microscopic Radioautographic Study on Protein Synthesis in Hepatocyte Mitochondria of Aging Mice
title Electron Microscopic Radioautographic Study on Protein Synthesis in Hepatocyte Mitochondria of Aging Mice
title_full Electron Microscopic Radioautographic Study on Protein Synthesis in Hepatocyte Mitochondria of Aging Mice
title_fullStr Electron Microscopic Radioautographic Study on Protein Synthesis in Hepatocyte Mitochondria of Aging Mice
title_full_unstemmed Electron Microscopic Radioautographic Study on Protein Synthesis in Hepatocyte Mitochondria of Aging Mice
title_short Electron Microscopic Radioautographic Study on Protein Synthesis in Hepatocyte Mitochondria of Aging Mice
title_sort electron microscopic radioautographic study on protein synthesis in hepatocyte mitochondria of aging mice
topic Review Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5917294/
https://www.ncbi.nlm.nih.gov/pubmed/17173177
http://dx.doi.org/10.1100/tsw.2006.265
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