Rostrocaudal Areal Patterning of Human PSC-Derived Cortical Neurons by FGF8 Signaling

The cerebral cortex is subdivided into distinct areas that have particular functions. The rostrocaudal (R-C) gradient of fibroblast growth factor 8 (FGF8) signaling defines this areal identity during neural development. In this study, we recapitulated cortical R-C patterning in human pluripotent ste...

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Autores principales: Imaizumi, Kent, Fujimori, Koki, Ishii, Seiji, Otomo, Asako, Hosoi, Yasushi, Miyajima, Hiroaki, Warita, Hitoshi, Aoki, Masashi, Hadano, Shinji, Akamatsu, Wado, Okano, Hideyuki
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Society for Neuroscience 2018
Materias:
New Research
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5917473/
https://www.ncbi.nlm.nih.gov/pubmed/29707616
http://dx.doi.org/10.1523/ENEURO.0368-17.2018
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author Imaizumi, Kent
Fujimori, Koki
Ishii, Seiji
Otomo, Asako
Hosoi, Yasushi
Miyajima, Hiroaki
Warita, Hitoshi
Aoki, Masashi
Hadano, Shinji
Akamatsu, Wado
Okano, Hideyuki
author_facet Imaizumi, Kent
Fujimori, Koki
Ishii, Seiji
Otomo, Asako
Hosoi, Yasushi
Miyajima, Hiroaki
Warita, Hitoshi
Aoki, Masashi
Hadano, Shinji
Akamatsu, Wado
Okano, Hideyuki
author_sort Imaizumi, Kent
collection PubMed
description The cerebral cortex is subdivided into distinct areas that have particular functions. The rostrocaudal (R-C) gradient of fibroblast growth factor 8 (FGF8) signaling defines this areal identity during neural development. In this study, we recapitulated cortical R-C patterning in human pluripotent stem cell (PSC) cultures. Modulation of FGF8 signaling appropriately regulated the R-C markers, and the patterns of global gene expression resembled those of the corresponding areas of human fetal brains. Furthermore, we demonstrated the utility of this culture system in modeling the area-specific forebrain phenotypes [presumptive upper motor neuron (UMN) phenotypes] of amyotrophic lateral sclerosis (ALS). We anticipate that our culture system will contribute to studies of human neurodevelopment and neurological disease modeling.
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spelling pubmed-59174732018-04-27 Rostrocaudal Areal Patterning of Human PSC-Derived Cortical Neurons by FGF8 Signaling Imaizumi, Kent Fujimori, Koki Ishii, Seiji Otomo, Asako Hosoi, Yasushi Miyajima, Hiroaki Warita, Hitoshi Aoki, Masashi Hadano, Shinji Akamatsu, Wado Okano, Hideyuki eNeuro New Research The cerebral cortex is subdivided into distinct areas that have particular functions. The rostrocaudal (R-C) gradient of fibroblast growth factor 8 (FGF8) signaling defines this areal identity during neural development. In this study, we recapitulated cortical R-C patterning in human pluripotent stem cell (PSC) cultures. Modulation of FGF8 signaling appropriately regulated the R-C markers, and the patterns of global gene expression resembled those of the corresponding areas of human fetal brains. Furthermore, we demonstrated the utility of this culture system in modeling the area-specific forebrain phenotypes [presumptive upper motor neuron (UMN) phenotypes] of amyotrophic lateral sclerosis (ALS). We anticipate that our culture system will contribute to studies of human neurodevelopment and neurological disease modeling. Society for Neuroscience 2018-04-26 /pmc/articles/PMC5917473/ /pubmed/29707616 http://dx.doi.org/10.1523/ENEURO.0368-17.2018 Text en Copyright © 2018 Imaizumi et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.
spellingShingle New Research
Imaizumi, Kent
Fujimori, Koki
Ishii, Seiji
Otomo, Asako
Hosoi, Yasushi
Miyajima, Hiroaki
Warita, Hitoshi
Aoki, Masashi
Hadano, Shinji
Akamatsu, Wado
Okano, Hideyuki
Rostrocaudal Areal Patterning of Human PSC-Derived Cortical Neurons by FGF8 Signaling
title Rostrocaudal Areal Patterning of Human PSC-Derived Cortical Neurons by FGF8 Signaling
title_full Rostrocaudal Areal Patterning of Human PSC-Derived Cortical Neurons by FGF8 Signaling
title_fullStr Rostrocaudal Areal Patterning of Human PSC-Derived Cortical Neurons by FGF8 Signaling
title_full_unstemmed Rostrocaudal Areal Patterning of Human PSC-Derived Cortical Neurons by FGF8 Signaling
title_short Rostrocaudal Areal Patterning of Human PSC-Derived Cortical Neurons by FGF8 Signaling
title_sort rostrocaudal areal patterning of human psc-derived cortical neurons by fgf8 signaling
topic New Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5917473/
https://www.ncbi.nlm.nih.gov/pubmed/29707616
http://dx.doi.org/10.1523/ENEURO.0368-17.2018
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