DIFFERENTIAL EXPRESSION OF CARCINOEMBRYONIC ANTIGEN AND NONSPECIFIC CROSSREACTING ANTIGEN GENES IN HUMAN COLON ADENOCARCINOMAS AND NORMAL COLON MUCOSA

The level of mRNA for carcinoembryonic antigen (CEA) and nonspecific crossreacting antigen (NCA) in human colon adenocarcinomas and normal colon mucosa was analyzed by Northern blot hybridization using as probes (32)P‐labeled CEA cDNA and synthetic oligodeoxyribonucleotides specific to CEA and NCA m...

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Detalles Bibliográficos
Autores principales: Sato, Chieko, Miyaki, Michiko, Oikawa, Shinzo, Nakazato, Hiroshi, Kosaki, Goro
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Blackwell Publishing Ltd 1988
Materias:
Rapid Communication
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5917497/
https://www.ncbi.nlm.nih.gov/pubmed/3133333
http://dx.doi.org/10.1111/j.1349-7006.1988.tb01610.x
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author Sato, Chieko
Miyaki, Michiko
Oikawa, Shinzo
Nakazato, Hiroshi
Kosaki, Goro
author_facet Sato, Chieko
Miyaki, Michiko
Oikawa, Shinzo
Nakazato, Hiroshi
Kosaki, Goro
author_sort Sato, Chieko
collection PubMed
description The level of mRNA for carcinoembryonic antigen (CEA) and nonspecific crossreacting antigen (NCA) in human colon adenocarcinomas and normal colon mucosa was analyzed by Northern blot hybridization using as probes (32)P‐labeled CEA cDNA and synthetic oligodeoxyribonucleotides specific to CEA and NCA mRNA sequences. The major 3.5‐kb mRNA and a minor 4.2‐kb mRNA are shown to be CEA‐specific and expressed in both tissues, albeit at slightly different degrees, suggesting that the expression of CEA is regulated posttranscriptionally. Another minor mRNA of 2.9 kb is NCA‐specific and expressed predominantly in cancerous tissues, suggesting its usefulness as a marker for colon cancer.
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spelling pubmed-59174972018-05-11 DIFFERENTIAL EXPRESSION OF CARCINOEMBRYONIC ANTIGEN AND NONSPECIFIC CROSSREACTING ANTIGEN GENES IN HUMAN COLON ADENOCARCINOMAS AND NORMAL COLON MUCOSA Sato, Chieko Miyaki, Michiko Oikawa, Shinzo Nakazato, Hiroshi Kosaki, Goro Jpn J Cancer Res Rapid Communication The level of mRNA for carcinoembryonic antigen (CEA) and nonspecific crossreacting antigen (NCA) in human colon adenocarcinomas and normal colon mucosa was analyzed by Northern blot hybridization using as probes (32)P‐labeled CEA cDNA and synthetic oligodeoxyribonucleotides specific to CEA and NCA mRNA sequences. The major 3.5‐kb mRNA and a minor 4.2‐kb mRNA are shown to be CEA‐specific and expressed in both tissues, albeit at slightly different degrees, suggesting that the expression of CEA is regulated posttranscriptionally. Another minor mRNA of 2.9 kb is NCA‐specific and expressed predominantly in cancerous tissues, suggesting its usefulness as a marker for colon cancer. Blackwell Publishing Ltd 1988-04 /pmc/articles/PMC5917497/ /pubmed/3133333 http://dx.doi.org/10.1111/j.1349-7006.1988.tb01610.x Text en
spellingShingle Rapid Communication
Sato, Chieko
Miyaki, Michiko
Oikawa, Shinzo
Nakazato, Hiroshi
Kosaki, Goro
DIFFERENTIAL EXPRESSION OF CARCINOEMBRYONIC ANTIGEN AND NONSPECIFIC CROSSREACTING ANTIGEN GENES IN HUMAN COLON ADENOCARCINOMAS AND NORMAL COLON MUCOSA
title DIFFERENTIAL EXPRESSION OF CARCINOEMBRYONIC ANTIGEN AND NONSPECIFIC CROSSREACTING ANTIGEN GENES IN HUMAN COLON ADENOCARCINOMAS AND NORMAL COLON MUCOSA
title_full DIFFERENTIAL EXPRESSION OF CARCINOEMBRYONIC ANTIGEN AND NONSPECIFIC CROSSREACTING ANTIGEN GENES IN HUMAN COLON ADENOCARCINOMAS AND NORMAL COLON MUCOSA
title_fullStr DIFFERENTIAL EXPRESSION OF CARCINOEMBRYONIC ANTIGEN AND NONSPECIFIC CROSSREACTING ANTIGEN GENES IN HUMAN COLON ADENOCARCINOMAS AND NORMAL COLON MUCOSA
title_full_unstemmed DIFFERENTIAL EXPRESSION OF CARCINOEMBRYONIC ANTIGEN AND NONSPECIFIC CROSSREACTING ANTIGEN GENES IN HUMAN COLON ADENOCARCINOMAS AND NORMAL COLON MUCOSA
title_short DIFFERENTIAL EXPRESSION OF CARCINOEMBRYONIC ANTIGEN AND NONSPECIFIC CROSSREACTING ANTIGEN GENES IN HUMAN COLON ADENOCARCINOMAS AND NORMAL COLON MUCOSA
title_sort differential expression of carcinoembryonic antigen and nonspecific crossreacting antigen genes in human colon adenocarcinomas and normal colon mucosa
topic Rapid Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5917497/
https://www.ncbi.nlm.nih.gov/pubmed/3133333
http://dx.doi.org/10.1111/j.1349-7006.1988.tb01610.x
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