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Coalition of E-cadherin and vascular endothelial growth factor expression in predicting malignant transformation in common oral potentially malignant disorders

BACKGROUND: Reduced E-cadherin expression and increased VEGF expression is known to be involved in tissue growth and transformation of Oral Potentially Malignant Disorders (OPMDs) and has been correlated with their differing histological grades in numerous studies. AIM: To evaluate Immunohistochemic...

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Autores principales: Sharada, P, Swaminathan, Uma, Nagamalini, BR, Kumar, K Vinod, Ashwini, BK, Lavanya, VLN
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Medknow Publications & Media Pvt Ltd 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5917540/
https://www.ncbi.nlm.nih.gov/pubmed/29731555
http://dx.doi.org/10.4103/jomfp.JOMFP_13_18
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author Sharada, P
Swaminathan, Uma
Nagamalini, BR
Kumar, K Vinod
Ashwini, BK
Lavanya, VLN
author_facet Sharada, P
Swaminathan, Uma
Nagamalini, BR
Kumar, K Vinod
Ashwini, BK
Lavanya, VLN
author_sort Sharada, P
collection PubMed
description BACKGROUND: Reduced E-cadherin expression and increased VEGF expression is known to be involved in tissue growth and transformation of Oral Potentially Malignant Disorders (OPMDs) and has been correlated with their differing histological grades in numerous studies. AIM: To evaluate Immunohistochemical (IHC) expression of both E-cadherin and VEGF in predicting the malignant transformation potential of common OPMDs. MATERIALS AND METHODS: Ten cases each of Normal Oral mucosa (NOM), Mild Oral Epithelial Dysplasia (OED), Moderate OED, Severe OED, Oral Submucous Fibrosis, (OSMF) and Oral Squamous Cell Carcinoma (OSCC) were stained and evaluated for the expression of Ecadherin and VEGF. Quick score (QS) for expression intensity in all epithelial layers was calculated for both markers and results statistically analysed using Kruskal –Wallis ANOVA and Mann-Whitney “U” test. RESULTS: E-cadherin expression was continuous and membranous in all the layers of NOM and reduced with progressing grades of OED to OSCC. In OSMF, expression was intermediate between moderate and severe OED. VEGF expression increased as the disease progressed from normal to increasing grades of OED to malignancy. In OSMF, expression was similar to that in mild OED. VEGF, E-cadherin expression for basal and parabasilar cells showed a strong statistically significant negative correlation in NOM. A very strong statistically significant positive correlation with perfect monotonic relation was noted in superficial cells in severe OED group and OSCC group. CONCLUSION: E-Cadherin and VEGF could be used as combination markers to predict the potential risk for malignant transformation in OEDs.
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spelling pubmed-59175402018-05-04 Coalition of E-cadherin and vascular endothelial growth factor expression in predicting malignant transformation in common oral potentially malignant disorders Sharada, P Swaminathan, Uma Nagamalini, BR Kumar, K Vinod Ashwini, BK Lavanya, VLN J Oral Maxillofac Pathol Original Article BACKGROUND: Reduced E-cadherin expression and increased VEGF expression is known to be involved in tissue growth and transformation of Oral Potentially Malignant Disorders (OPMDs) and has been correlated with their differing histological grades in numerous studies. AIM: To evaluate Immunohistochemical (IHC) expression of both E-cadherin and VEGF in predicting the malignant transformation potential of common OPMDs. MATERIALS AND METHODS: Ten cases each of Normal Oral mucosa (NOM), Mild Oral Epithelial Dysplasia (OED), Moderate OED, Severe OED, Oral Submucous Fibrosis, (OSMF) and Oral Squamous Cell Carcinoma (OSCC) were stained and evaluated for the expression of Ecadherin and VEGF. Quick score (QS) for expression intensity in all epithelial layers was calculated for both markers and results statistically analysed using Kruskal –Wallis ANOVA and Mann-Whitney “U” test. RESULTS: E-cadherin expression was continuous and membranous in all the layers of NOM and reduced with progressing grades of OED to OSCC. In OSMF, expression was intermediate between moderate and severe OED. VEGF expression increased as the disease progressed from normal to increasing grades of OED to malignancy. In OSMF, expression was similar to that in mild OED. VEGF, E-cadherin expression for basal and parabasilar cells showed a strong statistically significant negative correlation in NOM. A very strong statistically significant positive correlation with perfect monotonic relation was noted in superficial cells in severe OED group and OSCC group. CONCLUSION: E-Cadherin and VEGF could be used as combination markers to predict the potential risk for malignant transformation in OEDs. Medknow Publications & Media Pvt Ltd 2018 /pmc/articles/PMC5917540/ /pubmed/29731555 http://dx.doi.org/10.4103/jomfp.JOMFP_13_18 Text en Copyright: © 2018 Journal of Oral and Maxillofacial Pathology http://creativecommons.org/licenses/by-nc-sa/4.0 This is an open access journal, and articles are distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 4.0 License, which allows others to remix, tweak, and build upon the work non-commercially, as long as appropriate credit is given and the new creations are licensed under the identical terms.
spellingShingle Original Article
Sharada, P
Swaminathan, Uma
Nagamalini, BR
Kumar, K Vinod
Ashwini, BK
Lavanya, VLN
Coalition of E-cadherin and vascular endothelial growth factor expression in predicting malignant transformation in common oral potentially malignant disorders
title Coalition of E-cadherin and vascular endothelial growth factor expression in predicting malignant transformation in common oral potentially malignant disorders
title_full Coalition of E-cadherin and vascular endothelial growth factor expression in predicting malignant transformation in common oral potentially malignant disorders
title_fullStr Coalition of E-cadherin and vascular endothelial growth factor expression in predicting malignant transformation in common oral potentially malignant disorders
title_full_unstemmed Coalition of E-cadherin and vascular endothelial growth factor expression in predicting malignant transformation in common oral potentially malignant disorders
title_short Coalition of E-cadherin and vascular endothelial growth factor expression in predicting malignant transformation in common oral potentially malignant disorders
title_sort coalition of e-cadherin and vascular endothelial growth factor expression in predicting malignant transformation in common oral potentially malignant disorders
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5917540/
https://www.ncbi.nlm.nih.gov/pubmed/29731555
http://dx.doi.org/10.4103/jomfp.JOMFP_13_18
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