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Sandwich Radioimmunometric Assay with Murine Monoclonal Antibody, NCC‐ST‐439, for Serological Diagnosis of Human Cancers

Sandwich radioimmunometric assay (RIA) for a new tumor‐associated carbohydrate antigen defined by a monoclonal antibody (MoAb), NCC‐ST‐439, was developed and the antigen levels were determined in sera of normal donors, and patients with various malignant and non‐malignant disorders. In normal donors...

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Autores principales: Sugano, Kokichi, Ohkura, Hisanao, Maruyama, Takashi, Watanabe, Masahiko, Hirohashi, Setsuo, Shimosato, Yukio, Kodaira, Susumu, Abe, Osahiko
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Blackwell Publishing Ltd 1988
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5917558/
https://www.ncbi.nlm.nih.gov/pubmed/3136114
http://dx.doi.org/10.1111/j.1349-7006.1988.tb00031.x
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author Sugano, Kokichi
Ohkura, Hisanao
Maruyama, Takashi
Watanabe, Masahiko
Hirohashi, Setsuo
Shimosato, Yukio
Kodaira, Susumu
Abe, Osahiko
author_facet Sugano, Kokichi
Ohkura, Hisanao
Maruyama, Takashi
Watanabe, Masahiko
Hirohashi, Setsuo
Shimosato, Yukio
Kodaira, Susumu
Abe, Osahiko
author_sort Sugano, Kokichi
collection PubMed
description Sandwich radioimmunometric assay (RIA) for a new tumor‐associated carbohydrate antigen defined by a monoclonal antibody (MoAb), NCC‐ST‐439, was developed and the antigen levels were determined in sera of normal donors, and patients with various malignant and non‐malignant disorders. In normal donors, 97.0% (226/233) of sera were antigen‐negative (less than 12 units/ml) except for 7 serum samples from young females. In patients with malignant disorders, 34.2% (82/240) were antigen‐positive, in particular 64.0% (16/25) of patients with pancreatic carcinoma, 66.7% (16/24) of patients with recurrent colorectal carcinoma and 54,5% (6/11) of patients with recurrent breast carcinoma. In patients with non‐malignant disorders, 6.0% (7/116) were antigen‐positive. The positive rate in benign hepatobiliary disorders, including gallstones, hepatitis and liver cirrhosis, was especially low at 4.3% (1/23). We concluded that determination of serum NCC‐ST‐439 antigen would be useful in serodiagnosis of carcinoma patients.
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spelling pubmed-59175582018-05-11 Sandwich Radioimmunometric Assay with Murine Monoclonal Antibody, NCC‐ST‐439, for Serological Diagnosis of Human Cancers Sugano, Kokichi Ohkura, Hisanao Maruyama, Takashi Watanabe, Masahiko Hirohashi, Setsuo Shimosato, Yukio Kodaira, Susumu Abe, Osahiko Jpn J Cancer Res Article Sandwich radioimmunometric assay (RIA) for a new tumor‐associated carbohydrate antigen defined by a monoclonal antibody (MoAb), NCC‐ST‐439, was developed and the antigen levels were determined in sera of normal donors, and patients with various malignant and non‐malignant disorders. In normal donors, 97.0% (226/233) of sera were antigen‐negative (less than 12 units/ml) except for 7 serum samples from young females. In patients with malignant disorders, 34.2% (82/240) were antigen‐positive, in particular 64.0% (16/25) of patients with pancreatic carcinoma, 66.7% (16/24) of patients with recurrent colorectal carcinoma and 54,5% (6/11) of patients with recurrent breast carcinoma. In patients with non‐malignant disorders, 6.0% (7/116) were antigen‐positive. The positive rate in benign hepatobiliary disorders, including gallstones, hepatitis and liver cirrhosis, was especially low at 4.3% (1/23). We concluded that determination of serum NCC‐ST‐439 antigen would be useful in serodiagnosis of carcinoma patients. Blackwell Publishing Ltd 1988-05 /pmc/articles/PMC5917558/ /pubmed/3136114 http://dx.doi.org/10.1111/j.1349-7006.1988.tb00031.x Text en
spellingShingle Article
Sugano, Kokichi
Ohkura, Hisanao
Maruyama, Takashi
Watanabe, Masahiko
Hirohashi, Setsuo
Shimosato, Yukio
Kodaira, Susumu
Abe, Osahiko
Sandwich Radioimmunometric Assay with Murine Monoclonal Antibody, NCC‐ST‐439, for Serological Diagnosis of Human Cancers
title Sandwich Radioimmunometric Assay with Murine Monoclonal Antibody, NCC‐ST‐439, for Serological Diagnosis of Human Cancers
title_full Sandwich Radioimmunometric Assay with Murine Monoclonal Antibody, NCC‐ST‐439, for Serological Diagnosis of Human Cancers
title_fullStr Sandwich Radioimmunometric Assay with Murine Monoclonal Antibody, NCC‐ST‐439, for Serological Diagnosis of Human Cancers
title_full_unstemmed Sandwich Radioimmunometric Assay with Murine Monoclonal Antibody, NCC‐ST‐439, for Serological Diagnosis of Human Cancers
title_short Sandwich Radioimmunometric Assay with Murine Monoclonal Antibody, NCC‐ST‐439, for Serological Diagnosis of Human Cancers
title_sort sandwich radioimmunometric assay with murine monoclonal antibody, ncc‐st‐439, for serological diagnosis of human cancers
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5917558/
https://www.ncbi.nlm.nih.gov/pubmed/3136114
http://dx.doi.org/10.1111/j.1349-7006.1988.tb00031.x
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