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Induction of γ‐Interferon by Avarol in Human Peripheral Blood Lymphocytes

Avarol is a cytostatic and anti‐human immunodeficiency virus (HIV) agent. In this study, the avarol caused induction of γ‐interferon (IFN‐γ) in buffy coat cells (human peripheral blood lymphocytes) is demonstrated by immunological and molecular biological techniques. IFN‐γ production was detected af...

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Autores principales: Voth, Rita, Rossol, Siegbert, Hess, Georg, Laubenstein, Hans P., Büschenfelde, Karl‐H. Meyer zum, Schröder, Heinz C., Bachmann, Michael, Reuter, Petra, Müller, Werner E. G.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Blackwell Publishing Ltd 1988
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5917560/
https://www.ncbi.nlm.nih.gov/pubmed/3136118
http://dx.doi.org/10.1111/j.1349-7006.1988.tb00035.x
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author Voth, Rita
Rossol, Siegbert
Hess, Georg
Laubenstein, Hans P.
Büschenfelde, Karl‐H. Meyer zum
Schröder, Heinz C.
Bachmann, Michael
Reuter, Petra
Müller, Werner E. G.
author_facet Voth, Rita
Rossol, Siegbert
Hess, Georg
Laubenstein, Hans P.
Büschenfelde, Karl‐H. Meyer zum
Schröder, Heinz C.
Bachmann, Michael
Reuter, Petra
Müller, Werner E. G.
author_sort Voth, Rita
collection PubMed
description Avarol is a cytostatic and anti‐human immunodeficiency virus (HIV) agent. In this study, the avarol caused induction of γ‐interferon (IFN‐γ) in buffy coat cells (human peripheral blood lymphocytes) is demonstrated by immunological and molecular biological techniques. IFN‐γ production was detected after a 24‐hr incubation period with avarol; maximal production was obtained after 5 days in the presence of the optimal avarol concentration of 0.75 μg/ml. Blotting experiments using human IFN‐γ cDNA and β‐actin cDNA containing plasmids showed that in the absence of avarol no IFN‐γ transcripts were present in lymphocytes. Already after a 24‐hr incubation with avarol, IFN‐γ gene induction was detected, and maximal induction was found after a 5‐day incubation period. The enhanced IFN‐γ production seems to be caused by a change at the transcriptional and/or post‐transcriptional level, but not during subsequent nucleocyto‐plasmic transport of mRNA. This molecular event is specific, at least in relation to the expression of the β‐actin gene. Our studies demonstrate that avarol displays, besides its potential anti‐tumor and anti‐HIV activity, a potential immunomodulating effect.
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spelling pubmed-59175602018-05-11 Induction of γ‐Interferon by Avarol in Human Peripheral Blood Lymphocytes Voth, Rita Rossol, Siegbert Hess, Georg Laubenstein, Hans P. Büschenfelde, Karl‐H. Meyer zum Schröder, Heinz C. Bachmann, Michael Reuter, Petra Müller, Werner E. G. Jpn J Cancer Res Article Avarol is a cytostatic and anti‐human immunodeficiency virus (HIV) agent. In this study, the avarol caused induction of γ‐interferon (IFN‐γ) in buffy coat cells (human peripheral blood lymphocytes) is demonstrated by immunological and molecular biological techniques. IFN‐γ production was detected after a 24‐hr incubation period with avarol; maximal production was obtained after 5 days in the presence of the optimal avarol concentration of 0.75 μg/ml. Blotting experiments using human IFN‐γ cDNA and β‐actin cDNA containing plasmids showed that in the absence of avarol no IFN‐γ transcripts were present in lymphocytes. Already after a 24‐hr incubation with avarol, IFN‐γ gene induction was detected, and maximal induction was found after a 5‐day incubation period. The enhanced IFN‐γ production seems to be caused by a change at the transcriptional and/or post‐transcriptional level, but not during subsequent nucleocyto‐plasmic transport of mRNA. This molecular event is specific, at least in relation to the expression of the β‐actin gene. Our studies demonstrate that avarol displays, besides its potential anti‐tumor and anti‐HIV activity, a potential immunomodulating effect. Blackwell Publishing Ltd 1988-05 /pmc/articles/PMC5917560/ /pubmed/3136118 http://dx.doi.org/10.1111/j.1349-7006.1988.tb00035.x Text en
spellingShingle Article
Voth, Rita
Rossol, Siegbert
Hess, Georg
Laubenstein, Hans P.
Büschenfelde, Karl‐H. Meyer zum
Schröder, Heinz C.
Bachmann, Michael
Reuter, Petra
Müller, Werner E. G.
Induction of γ‐Interferon by Avarol in Human Peripheral Blood Lymphocytes
title Induction of γ‐Interferon by Avarol in Human Peripheral Blood Lymphocytes
title_full Induction of γ‐Interferon by Avarol in Human Peripheral Blood Lymphocytes
title_fullStr Induction of γ‐Interferon by Avarol in Human Peripheral Blood Lymphocytes
title_full_unstemmed Induction of γ‐Interferon by Avarol in Human Peripheral Blood Lymphocytes
title_short Induction of γ‐Interferon by Avarol in Human Peripheral Blood Lymphocytes
title_sort induction of γ‐interferon by avarol in human peripheral blood lymphocytes
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5917560/
https://www.ncbi.nlm.nih.gov/pubmed/3136118
http://dx.doi.org/10.1111/j.1349-7006.1988.tb00035.x
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