Cargando…
Establishment of Megakaryoblastic Cell Lines by Coinfection of Abelson Murine Leukemia Virus and Recombinant SV40‐retrovirus
Murine embryonic cells including yolk sac prepared from 8‐day embryos were co‐infected with Abelson murine leukemia virus (A‐MuLV) and/or a recombinant retrovirus containing large T and small t antigens, and early region of simian virus 40 (M‐SV40). By coinfection with A‐MuLV and M‐SV40, megakaryobl...
Autores principales: | , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Blackwell Publishing Ltd
1988
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5917582/ https://www.ncbi.nlm.nih.gov/pubmed/2842281 http://dx.doi.org/10.1111/j.1349-7006.1988.tb02229.x |
_version_ | 1783317242917683200 |
---|---|
author | Kajigaya, Sachiko Suda, Toshio Suda, Junko Eguchi, Mitsuoki Moroi, Masaaki Sudo, Tetsuo Saito, Masaki Miira, Yasusada |
author_facet | Kajigaya, Sachiko Suda, Toshio Suda, Junko Eguchi, Mitsuoki Moroi, Masaaki Sudo, Tetsuo Saito, Masaki Miira, Yasusada |
author_sort | Kajigaya, Sachiko |
collection | PubMed |
description | Murine embryonic cells including yolk sac prepared from 8‐day embryos were co‐infected with Abelson murine leukemia virus (A‐MuLV) and/or a recombinant retrovirus containing large T and small t antigens, and early region of simian virus 40 (M‐SV40). By coinfection with A‐MuLV and M‐SV40, megakaryoblastic cells were obtained in addition to mast cells and fibroblastic cells. However, following infection with A‐MuLV or M‐SV40 alone, no megakaryoblastic cells were detected, although mast cells and/or fibroblastic cells developed. The same results were obtained in several experiments. By single‐cell transfer, 6 acetyl‐cholinesterase (AchE)‐positive clonal cell lines were established. Characteristics of megakaryocytes, such as AchE, glycoproteins lIb and IlIa, and platelet peroxidase were detected in two representative cells (C1 and C8). More significant changes expressing differentiation were observed following treatment with phorbol myristate acetate or pokeweed mitogen‐stimulated murine spleen cell conditioned medium, although release of platelets was not observed. This is the first report showing development of megakaryocytic cells as the result of coinfection with retroviruses. |
format | Online Article Text |
id | pubmed-5917582 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 1988 |
publisher | Blackwell Publishing Ltd |
record_format | MEDLINE/PubMed |
spelling | pubmed-59175822018-05-11 Establishment of Megakaryoblastic Cell Lines by Coinfection of Abelson Murine Leukemia Virus and Recombinant SV40‐retrovirus Kajigaya, Sachiko Suda, Toshio Suda, Junko Eguchi, Mitsuoki Moroi, Masaaki Sudo, Tetsuo Saito, Masaki Miira, Yasusada Jpn J Cancer Res Article Murine embryonic cells including yolk sac prepared from 8‐day embryos were co‐infected with Abelson murine leukemia virus (A‐MuLV) and/or a recombinant retrovirus containing large T and small t antigens, and early region of simian virus 40 (M‐SV40). By coinfection with A‐MuLV and M‐SV40, megakaryoblastic cells were obtained in addition to mast cells and fibroblastic cells. However, following infection with A‐MuLV or M‐SV40 alone, no megakaryoblastic cells were detected, although mast cells and/or fibroblastic cells developed. The same results were obtained in several experiments. By single‐cell transfer, 6 acetyl‐cholinesterase (AchE)‐positive clonal cell lines were established. Characteristics of megakaryocytes, such as AchE, glycoproteins lIb and IlIa, and platelet peroxidase were detected in two representative cells (C1 and C8). More significant changes expressing differentiation were observed following treatment with phorbol myristate acetate or pokeweed mitogen‐stimulated murine spleen cell conditioned medium, although release of platelets was not observed. This is the first report showing development of megakaryocytic cells as the result of coinfection with retroviruses. Blackwell Publishing Ltd 1988-06 /pmc/articles/PMC5917582/ /pubmed/2842281 http://dx.doi.org/10.1111/j.1349-7006.1988.tb02229.x Text en |
spellingShingle | Article Kajigaya, Sachiko Suda, Toshio Suda, Junko Eguchi, Mitsuoki Moroi, Masaaki Sudo, Tetsuo Saito, Masaki Miira, Yasusada Establishment of Megakaryoblastic Cell Lines by Coinfection of Abelson Murine Leukemia Virus and Recombinant SV40‐retrovirus |
title | Establishment of Megakaryoblastic Cell Lines by Coinfection of Abelson Murine Leukemia Virus and Recombinant SV40‐retrovirus |
title_full | Establishment of Megakaryoblastic Cell Lines by Coinfection of Abelson Murine Leukemia Virus and Recombinant SV40‐retrovirus |
title_fullStr | Establishment of Megakaryoblastic Cell Lines by Coinfection of Abelson Murine Leukemia Virus and Recombinant SV40‐retrovirus |
title_full_unstemmed | Establishment of Megakaryoblastic Cell Lines by Coinfection of Abelson Murine Leukemia Virus and Recombinant SV40‐retrovirus |
title_short | Establishment of Megakaryoblastic Cell Lines by Coinfection of Abelson Murine Leukemia Virus and Recombinant SV40‐retrovirus |
title_sort | establishment of megakaryoblastic cell lines by coinfection of abelson murine leukemia virus and recombinant sv40‐retrovirus |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5917582/ https://www.ncbi.nlm.nih.gov/pubmed/2842281 http://dx.doi.org/10.1111/j.1349-7006.1988.tb02229.x |
work_keys_str_mv | AT kajigayasachiko establishmentofmegakaryoblasticcelllinesbycoinfectionofabelsonmurineleukemiavirusandrecombinantsv40retrovirus AT sudatoshio establishmentofmegakaryoblasticcelllinesbycoinfectionofabelsonmurineleukemiavirusandrecombinantsv40retrovirus AT sudajunko establishmentofmegakaryoblasticcelllinesbycoinfectionofabelsonmurineleukemiavirusandrecombinantsv40retrovirus AT eguchimitsuoki establishmentofmegakaryoblasticcelllinesbycoinfectionofabelsonmurineleukemiavirusandrecombinantsv40retrovirus AT moroimasaaki establishmentofmegakaryoblasticcelllinesbycoinfectionofabelsonmurineleukemiavirusandrecombinantsv40retrovirus AT sudotetsuo establishmentofmegakaryoblasticcelllinesbycoinfectionofabelsonmurineleukemiavirusandrecombinantsv40retrovirus AT saitomasaki establishmentofmegakaryoblasticcelllinesbycoinfectionofabelsonmurineleukemiavirusandrecombinantsv40retrovirus AT miirayasusada establishmentofmegakaryoblasticcelllinesbycoinfectionofabelsonmurineleukemiavirusandrecombinantsv40retrovirus |