Isolation and Characterization of ras‐Transfected BALB/3T3 Clone Showing Morphological Transformation by 12‐O‐Tetradecanoyl‐phorbol‐13‐acetate

The transformation frequency of mouse BALB/3T3 cells was significantly enhanced after transfection with an activated ras oncogene (v‐Ha‐ras) followed by treatment with a tumor promoter, 12‐O‐tetradecanoyl‐phorbol‐13‐acetate (TPA), suggesting that the ras oncogene acted as an initiator in two‐stage c...

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Detalles Bibliográficos
Autores principales: Sasaki, Kiyoshi, Mizusawa, Hiroshi, Ishidate, Motoi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Blackwell Publishing Ltd 1988
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5917616/
https://www.ncbi.nlm.nih.gov/pubmed/3141328
http://dx.doi.org/10.1111/j.1349-7006.1988.tb00056.x
Descripción
Sumario:The transformation frequency of mouse BALB/3T3 cells was significantly enhanced after transfection with an activated ras oncogene (v‐Ha‐ras) followed by treatment with a tumor promoter, 12‐O‐tetradecanoyl‐phorbol‐13‐acetate (TPA), suggesting that the ras oncogene acted as an initiator in two‐stage carcinogenesis. A cell clone (Bhas42) containing the ras oncogene was isolated from the ras‐transfected BALB/3T3 cells. Bhas42 cells were flat and showed contact inhibition, but the addition of TPA to quiescent Bhas42 cultures resulted in a dramatic change of cell morphology to spindle shape, doubling of the cell population, and increased DNA synthesis.

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