Isolation and Characterization of ras‐Transfected BALB/3T3 Clone Showing Morphological Transformation by 12‐O‐Tetradecanoyl‐phorbol‐13‐acetate

The transformation frequency of mouse BALB/3T3 cells was significantly enhanced after transfection with an activated ras oncogene (v‐Ha‐ras) followed by treatment with a tumor promoter, 12‐O‐tetradecanoyl‐phorbol‐13‐acetate (TPA), suggesting that the ras oncogene acted as an initiator in two‐stage c...

Descripción completa

Detalles Bibliográficos
Autores principales: Sasaki, Kiyoshi, Mizusawa, Hiroshi, Ishidate, Motoi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Blackwell Publishing Ltd 1988
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5917616/
https://www.ncbi.nlm.nih.gov/pubmed/3141328
http://dx.doi.org/10.1111/j.1349-7006.1988.tb00056.x
_version_ 1783317250143420416
author Sasaki, Kiyoshi
Mizusawa, Hiroshi
Ishidate, Motoi
author_facet Sasaki, Kiyoshi
Mizusawa, Hiroshi
Ishidate, Motoi
author_sort Sasaki, Kiyoshi
collection PubMed
description The transformation frequency of mouse BALB/3T3 cells was significantly enhanced after transfection with an activated ras oncogene (v‐Ha‐ras) followed by treatment with a tumor promoter, 12‐O‐tetradecanoyl‐phorbol‐13‐acetate (TPA), suggesting that the ras oncogene acted as an initiator in two‐stage carcinogenesis. A cell clone (Bhas42) containing the ras oncogene was isolated from the ras‐transfected BALB/3T3 cells. Bhas42 cells were flat and showed contact inhibition, but the addition of TPA to quiescent Bhas42 cultures resulted in a dramatic change of cell morphology to spindle shape, doubling of the cell population, and increased DNA synthesis.
format Online
Article
Text
id pubmed-5917616
institution National Center for Biotechnology Information
language English
publishDate 1988
publisher Blackwell Publishing Ltd
record_format MEDLINE/PubMed
spelling pubmed-59176162018-05-11 Isolation and Characterization of ras‐Transfected BALB/3T3 Clone Showing Morphological Transformation by 12‐O‐Tetradecanoyl‐phorbol‐13‐acetate Sasaki, Kiyoshi Mizusawa, Hiroshi Ishidate, Motoi Jpn J Cancer Res Article The transformation frequency of mouse BALB/3T3 cells was significantly enhanced after transfection with an activated ras oncogene (v‐Ha‐ras) followed by treatment with a tumor promoter, 12‐O‐tetradecanoyl‐phorbol‐13‐acetate (TPA), suggesting that the ras oncogene acted as an initiator in two‐stage carcinogenesis. A cell clone (Bhas42) containing the ras oncogene was isolated from the ras‐transfected BALB/3T3 cells. Bhas42 cells were flat and showed contact inhibition, but the addition of TPA to quiescent Bhas42 cultures resulted in a dramatic change of cell morphology to spindle shape, doubling of the cell population, and increased DNA synthesis. Blackwell Publishing Ltd 1988-08 /pmc/articles/PMC5917616/ /pubmed/3141328 http://dx.doi.org/10.1111/j.1349-7006.1988.tb00056.x Text en
spellingShingle Article
Sasaki, Kiyoshi
Mizusawa, Hiroshi
Ishidate, Motoi
Isolation and Characterization of ras‐Transfected BALB/3T3 Clone Showing Morphological Transformation by 12‐O‐Tetradecanoyl‐phorbol‐13‐acetate
title Isolation and Characterization of ras‐Transfected BALB/3T3 Clone Showing Morphological Transformation by 12‐O‐Tetradecanoyl‐phorbol‐13‐acetate
title_full Isolation and Characterization of ras‐Transfected BALB/3T3 Clone Showing Morphological Transformation by 12‐O‐Tetradecanoyl‐phorbol‐13‐acetate
title_fullStr Isolation and Characterization of ras‐Transfected BALB/3T3 Clone Showing Morphological Transformation by 12‐O‐Tetradecanoyl‐phorbol‐13‐acetate
title_full_unstemmed Isolation and Characterization of ras‐Transfected BALB/3T3 Clone Showing Morphological Transformation by 12‐O‐Tetradecanoyl‐phorbol‐13‐acetate
title_short Isolation and Characterization of ras‐Transfected BALB/3T3 Clone Showing Morphological Transformation by 12‐O‐Tetradecanoyl‐phorbol‐13‐acetate
title_sort isolation and characterization of ras‐transfected balb/3t3 clone showing morphological transformation by 12‐o‐tetradecanoyl‐phorbol‐13‐acetate
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5917616/
https://www.ncbi.nlm.nih.gov/pubmed/3141328
http://dx.doi.org/10.1111/j.1349-7006.1988.tb00056.x
work_keys_str_mv AT sasakikiyoshi isolationandcharacterizationofrastransfectedbalb3t3cloneshowingmorphologicaltransformationby12otetradecanoylphorbol13acetate
AT mizusawahiroshi isolationandcharacterizationofrastransfectedbalb3t3cloneshowingmorphologicaltransformationby12otetradecanoylphorbol13acetate
AT ishidatemotoi isolationandcharacterizationofrastransfectedbalb3t3cloneshowingmorphologicaltransformationby12otetradecanoylphorbol13acetate

Ejemplares similares