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XENOGENIZATION OF TUMOR CELLS BY TRANSFECTION WITH PLASMID CONTAINING env GENE OF FRIEND LEUKEMIA VIRUS

A rat hepatocellular carcinoma cell line (cKDH‐8 cl‐11) showed decreased tumorigenicity after transfection with an envelope gene derived from a Friend leukemia virus (FV‐env gene). FV‐env gene product was found by indirect immunofluorescence staining to be expressed on the cell surface of the FV‐env...

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Autores principales: Sugiura, Chihiro, Itaya, Toshiyuki, Kondoh, Nobuo, Oikawa, Tsuneyuki, Kuzumaki, Noboru, Takeichi, Noritoshi, Hosokawa, Masuo, Kobayashi, Hiroshi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Blackwell Publishing Ltd 1988
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5917664/
https://www.ncbi.nlm.nih.gov/pubmed/3148594
http://dx.doi.org/10.1111/j.1349-7006.1988.tb01553.x
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author Sugiura, Chihiro
Itaya, Toshiyuki
Kondoh, Nobuo
Oikawa, Tsuneyuki
Kuzumaki, Noboru
Takeichi, Noritoshi
Hosokawa, Masuo
Kobayashi, Hiroshi
author_facet Sugiura, Chihiro
Itaya, Toshiyuki
Kondoh, Nobuo
Oikawa, Tsuneyuki
Kuzumaki, Noboru
Takeichi, Noritoshi
Hosokawa, Masuo
Kobayashi, Hiroshi
author_sort Sugiura, Chihiro
collection PubMed
description A rat hepatocellular carcinoma cell line (cKDH‐8 cl‐11) showed decreased tumorigenicity after transfection with an envelope gene derived from a Friend leukemia virus (FV‐env gene). FV‐env gene product was found by indirect immunofluorescence staining to be expressed on the cell surface of the FV‐env gene‐transfected cells. The FV‐env‐transfected cells (FV‐env cKDH‐8), however, grew well in X‐irradiated immunosuppressed rats, indicating that the reduction in tumorigenicity of the transfected cells is based on immunological reaction in the host. The rats which rejected FV‐env cKDH‐8 cells showed resistance to rechallenge with the parent cKDH‐8 cl‐11 tumor cells. These results suggest that the FV‐env gene product may elicit antitumor immunity against FV‐env cKDH‐8 cells in a host with a resultant reduction in the tumorigenicity of these cells.
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spelling pubmed-59176642018-05-11 XENOGENIZATION OF TUMOR CELLS BY TRANSFECTION WITH PLASMID CONTAINING env GENE OF FRIEND LEUKEMIA VIRUS Sugiura, Chihiro Itaya, Toshiyuki Kondoh, Nobuo Oikawa, Tsuneyuki Kuzumaki, Noboru Takeichi, Noritoshi Hosokawa, Masuo Kobayashi, Hiroshi Jpn J Cancer Res Rapid Communication A rat hepatocellular carcinoma cell line (cKDH‐8 cl‐11) showed decreased tumorigenicity after transfection with an envelope gene derived from a Friend leukemia virus (FV‐env gene). FV‐env gene product was found by indirect immunofluorescence staining to be expressed on the cell surface of the FV‐env gene‐transfected cells. The FV‐env‐transfected cells (FV‐env cKDH‐8), however, grew well in X‐irradiated immunosuppressed rats, indicating that the reduction in tumorigenicity of the transfected cells is based on immunological reaction in the host. The rats which rejected FV‐env cKDH‐8 cells showed resistance to rechallenge with the parent cKDH‐8 cl‐11 tumor cells. These results suggest that the FV‐env gene product may elicit antitumor immunity against FV‐env cKDH‐8 cells in a host with a resultant reduction in the tumorigenicity of these cells. Blackwell Publishing Ltd 1988-12 /pmc/articles/PMC5917664/ /pubmed/3148594 http://dx.doi.org/10.1111/j.1349-7006.1988.tb01553.x Text en
spellingShingle Rapid Communication
Sugiura, Chihiro
Itaya, Toshiyuki
Kondoh, Nobuo
Oikawa, Tsuneyuki
Kuzumaki, Noboru
Takeichi, Noritoshi
Hosokawa, Masuo
Kobayashi, Hiroshi
XENOGENIZATION OF TUMOR CELLS BY TRANSFECTION WITH PLASMID CONTAINING env GENE OF FRIEND LEUKEMIA VIRUS
title XENOGENIZATION OF TUMOR CELLS BY TRANSFECTION WITH PLASMID CONTAINING env GENE OF FRIEND LEUKEMIA VIRUS
title_full XENOGENIZATION OF TUMOR CELLS BY TRANSFECTION WITH PLASMID CONTAINING env GENE OF FRIEND LEUKEMIA VIRUS
title_fullStr XENOGENIZATION OF TUMOR CELLS BY TRANSFECTION WITH PLASMID CONTAINING env GENE OF FRIEND LEUKEMIA VIRUS
title_full_unstemmed XENOGENIZATION OF TUMOR CELLS BY TRANSFECTION WITH PLASMID CONTAINING env GENE OF FRIEND LEUKEMIA VIRUS
title_short XENOGENIZATION OF TUMOR CELLS BY TRANSFECTION WITH PLASMID CONTAINING env GENE OF FRIEND LEUKEMIA VIRUS
title_sort xenogenization of tumor cells by transfection with plasmid containing env gene of friend leukemia virus
topic Rapid Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5917664/
https://www.ncbi.nlm.nih.gov/pubmed/3148594
http://dx.doi.org/10.1111/j.1349-7006.1988.tb01553.x
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