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High Induction of Poly(ADP‐ribose) Polymerase Activity in Bleomycin‐resistant HeLa Cells

Poly(ADP‐ribose) polymerase activity was measured in bleomycin (BLM) ‐ resistant HeLa (HeLa‐BLM(r)) and the parental HeLa cells after BLM treatment. HeLa‐BLM(r) cells, which had been subcultured in growth medium containing 1 μg/ml of BLM, showed a 3.75‐fold higher enzyme activity than did HeLa cells...

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Detalles Bibliográficos
Autores principales: Urade, Masahiro, Sugi, Masakazu, Mima, Takashi, Ogura, Takafumi, Matsuya, Tokuzo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Blackwell Publishing Ltd 1989
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5917762/
https://www.ncbi.nlm.nih.gov/pubmed/2473971
http://dx.doi.org/10.1111/j.1349-7006.1989.tb02337.x
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author Urade, Masahiro
Sugi, Masakazu
Mima, Takashi
Ogura, Takafumi
Matsuya, Tokuzo
author_facet Urade, Masahiro
Sugi, Masakazu
Mima, Takashi
Ogura, Takafumi
Matsuya, Tokuzo
author_sort Urade, Masahiro
collection PubMed
description Poly(ADP‐ribose) polymerase activity was measured in bleomycin (BLM) ‐ resistant HeLa (HeLa‐BLM(r)) and the parental HeLa cells after BLM treatment. HeLa‐BLM(r) cells, which had been subcultured in growth medium containing 1 μg/ml of BLM, showed a 3.75‐fold higher enzyme activity than did HeLa cells, but this activity was decreased to the same level as that of HeLa cells after 48 h of BLM‐free cultivation. When HeLa and HeLa‐BLM(r) cells after a 48‐h cultivation in BLM‐free growth medium were treated with BLM, the enzyme activity was induced at a higher level (2.5–3.8 times) in HeLa‐BLM(r) than in HeLa cells and was inhibited markedly in HeLa‐BLM(r) and slightly in HeLa cells by nicotinamide, an inhibitor of this enzyme. The BLM‐induced cell killing by nicotinamide was highly potentiated (about 18 times) in HeLa‐BLM(r) as compared to HeLa cells.
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spelling pubmed-59177622018-05-11 High Induction of Poly(ADP‐ribose) Polymerase Activity in Bleomycin‐resistant HeLa Cells Urade, Masahiro Sugi, Masakazu Mima, Takashi Ogura, Takafumi Matsuya, Tokuzo Jpn J Cancer Res Article Poly(ADP‐ribose) polymerase activity was measured in bleomycin (BLM) ‐ resistant HeLa (HeLa‐BLM(r)) and the parental HeLa cells after BLM treatment. HeLa‐BLM(r) cells, which had been subcultured in growth medium containing 1 μg/ml of BLM, showed a 3.75‐fold higher enzyme activity than did HeLa cells, but this activity was decreased to the same level as that of HeLa cells after 48 h of BLM‐free cultivation. When HeLa and HeLa‐BLM(r) cells after a 48‐h cultivation in BLM‐free growth medium were treated with BLM, the enzyme activity was induced at a higher level (2.5–3.8 times) in HeLa‐BLM(r) than in HeLa cells and was inhibited markedly in HeLa‐BLM(r) and slightly in HeLa cells by nicotinamide, an inhibitor of this enzyme. The BLM‐induced cell killing by nicotinamide was highly potentiated (about 18 times) in HeLa‐BLM(r) as compared to HeLa cells. Blackwell Publishing Ltd 1989-05 /pmc/articles/PMC5917762/ /pubmed/2473971 http://dx.doi.org/10.1111/j.1349-7006.1989.tb02337.x Text en
spellingShingle Article
Urade, Masahiro
Sugi, Masakazu
Mima, Takashi
Ogura, Takafumi
Matsuya, Tokuzo
High Induction of Poly(ADP‐ribose) Polymerase Activity in Bleomycin‐resistant HeLa Cells
title High Induction of Poly(ADP‐ribose) Polymerase Activity in Bleomycin‐resistant HeLa Cells
title_full High Induction of Poly(ADP‐ribose) Polymerase Activity in Bleomycin‐resistant HeLa Cells
title_fullStr High Induction of Poly(ADP‐ribose) Polymerase Activity in Bleomycin‐resistant HeLa Cells
title_full_unstemmed High Induction of Poly(ADP‐ribose) Polymerase Activity in Bleomycin‐resistant HeLa Cells
title_short High Induction of Poly(ADP‐ribose) Polymerase Activity in Bleomycin‐resistant HeLa Cells
title_sort high induction of poly(adp‐ribose) polymerase activity in bleomycin‐resistant hela cells
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5917762/
https://www.ncbi.nlm.nih.gov/pubmed/2473971
http://dx.doi.org/10.1111/j.1349-7006.1989.tb02337.x
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