Construction of Mouse A9 Clones Containing a Single Human Chromosome Tagged with Neomycin‐resistance Gene via Microcell Fusion

Normal human fibroblasts (MRC‐5 or NTI‐4) were transfected with pSV2‐neo plasmid DNA. Fifty G418‐resistant fibroblast clones were isolated and independently fused to mouse A9 cells. The cell hybrids were selected and isolated in the medium containing G418 plus ouabaln. Since micronuclei were more ef...

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Detalles Bibliográficos
Autores principales: Koi, Minoru, Shimizu, Motoyuki, Morita, Hiroyuki, Yamada, Hideto, Oshimura, Mitsuo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Blackwell Publishing Ltd 1989
Materias:
Rapid Communication
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5917765/
https://www.ncbi.nlm.nih.gov/pubmed/2502516
http://dx.doi.org/10.1111/j.1349-7006.1989.tb02329.x

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5917765/
https://www.ncbi.nlm.nih.gov/pubmed/2502516
http://dx.doi.org/10.1111/j.1349-7006.1989.tb02329.x

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