Cargando…
In vitro Invasion of Endothelial Cell Monolayer by Rat Ascites Hepatoma Cells
To study the tissue preference of invasion, we developed an assay system for the invasion of endothelial cells as a modification of the previously established assay of tumor cell invasion of meso‐thelial cells. Rat ascites hepatoma cells (AH 130) that had been seeded on a monolayer of cultured endot...
Autores principales: | , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Blackwell Publishing Ltd
1989
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5917849/ https://www.ncbi.nlm.nih.gov/pubmed/2513297 http://dx.doi.org/10.1111/j.1349-7006.1989.tb01720.x |
_version_ | 1783317302576414720 |
---|---|
author | Ohigashi, Hiroaki Shinkai, Kiyoko Mukai, Mutsuko Ishikawa, Osamu Imaoka, Shingi Iwanaga, Takeshi Akedo, Hitoshi |
author_facet | Ohigashi, Hiroaki Shinkai, Kiyoko Mukai, Mutsuko Ishikawa, Osamu Imaoka, Shingi Iwanaga, Takeshi Akedo, Hitoshi |
author_sort | Ohigashi, Hiroaki |
collection | PubMed |
description | To study the tissue preference of invasion, we developed an assay system for the invasion of endothelial cells as a modification of the previously established assay of tumor cell invasion of meso‐thelial cells. Rat ascites hepatoma cells (AH 130) that had been seeded on a monolayer of cultured endothelial cells penetrated and formed tumor cell colonies under the monolayer. The penetration was time‐dependent and the number of penetrated tumor cells and colonies was proportional to the number of tumor cells seeded. Comparison of the in vitro tumor cell invasion of endothelial cell monolayer with that of cultured mesothelial cell layer showed that a clone from the tumor cells (CI‐30) which was highly penetrative into the mesothelial cell layer had only a limited ability to penetrate the endothelial cell layer. |
format | Online Article Text |
id | pubmed-5917849 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 1989 |
publisher | Blackwell Publishing Ltd |
record_format | MEDLINE/PubMed |
spelling | pubmed-59178492018-05-11 In vitro Invasion of Endothelial Cell Monolayer by Rat Ascites Hepatoma Cells Ohigashi, Hiroaki Shinkai, Kiyoko Mukai, Mutsuko Ishikawa, Osamu Imaoka, Shingi Iwanaga, Takeshi Akedo, Hitoshi Jpn J Cancer Res Rapid Communication To study the tissue preference of invasion, we developed an assay system for the invasion of endothelial cells as a modification of the previously established assay of tumor cell invasion of meso‐thelial cells. Rat ascites hepatoma cells (AH 130) that had been seeded on a monolayer of cultured endothelial cells penetrated and formed tumor cell colonies under the monolayer. The penetration was time‐dependent and the number of penetrated tumor cells and colonies was proportional to the number of tumor cells seeded. Comparison of the in vitro tumor cell invasion of endothelial cell monolayer with that of cultured mesothelial cell layer showed that a clone from the tumor cells (CI‐30) which was highly penetrative into the mesothelial cell layer had only a limited ability to penetrate the endothelial cell layer. Blackwell Publishing Ltd 1989-09 /pmc/articles/PMC5917849/ /pubmed/2513297 http://dx.doi.org/10.1111/j.1349-7006.1989.tb01720.x Text en |
spellingShingle | Rapid Communication Ohigashi, Hiroaki Shinkai, Kiyoko Mukai, Mutsuko Ishikawa, Osamu Imaoka, Shingi Iwanaga, Takeshi Akedo, Hitoshi In vitro Invasion of Endothelial Cell Monolayer by Rat Ascites Hepatoma Cells |
title | In vitro Invasion of Endothelial Cell Monolayer by Rat Ascites Hepatoma Cells |
title_full | In vitro Invasion of Endothelial Cell Monolayer by Rat Ascites Hepatoma Cells |
title_fullStr | In vitro Invasion of Endothelial Cell Monolayer by Rat Ascites Hepatoma Cells |
title_full_unstemmed | In vitro Invasion of Endothelial Cell Monolayer by Rat Ascites Hepatoma Cells |
title_short | In vitro Invasion of Endothelial Cell Monolayer by Rat Ascites Hepatoma Cells |
title_sort | in vitro invasion of endothelial cell monolayer by rat ascites hepatoma cells |
topic | Rapid Communication |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5917849/ https://www.ncbi.nlm.nih.gov/pubmed/2513297 http://dx.doi.org/10.1111/j.1349-7006.1989.tb01720.x |
work_keys_str_mv | AT ohigashihiroaki invitroinvasionofendothelialcellmonolayerbyratasciteshepatomacells AT shinkaikiyoko invitroinvasionofendothelialcellmonolayerbyratasciteshepatomacells AT mukaimutsuko invitroinvasionofendothelialcellmonolayerbyratasciteshepatomacells AT ishikawaosamu invitroinvasionofendothelialcellmonolayerbyratasciteshepatomacells AT imaokashingi invitroinvasionofendothelialcellmonolayerbyratasciteshepatomacells AT iwanagatakeshi invitroinvasionofendothelialcellmonolayerbyratasciteshepatomacells AT akedohitoshi invitroinvasionofendothelialcellmonolayerbyratasciteshepatomacells |