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Density of GM3 with Normal Primary Structure Determines Mouse Melanoma Antigenicity; a New Concept of Tumor Antigen
We attempted to induce anti‐melanoma cytotoxic T cells (CTL) and suppressor T cells (Ts) inhibiting CTL generation by using liposomes carrying various densities of GM3 as tumor antigens. We found that liposomes carrying 6–16 mol% of GM3 with normal primary structure successfully generated anti‐melan...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Blackwell Publishing Ltd
1989
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5917884/ https://www.ncbi.nlm.nih.gov/pubmed/2533193 http://dx.doi.org/10.1111/j.1349-7006.1989.tb01638.x |
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author | Harada, Yoshitada Sakatsume, Minoru Nores, Gustavo A. Hakomori, Sen‐itiroh Taniguchi, Masaru |
author_facet | Harada, Yoshitada Sakatsume, Minoru Nores, Gustavo A. Hakomori, Sen‐itiroh Taniguchi, Masaru |
author_sort | Harada, Yoshitada |
collection | PubMed |
description | We attempted to induce anti‐melanoma cytotoxic T cells (CTL) and suppressor T cells (Ts) inhibiting CTL generation by using liposomes carrying various densities of GM3 as tumor antigens. We found that liposomes carrying 6–16 mol% of GM3 with normal primary structure successfully generated anti‐melanoma CTL and suppressor T cells, while liposomes with GM3 outside this range had little or no such activity. Anti‐melanoma CTL induced by GM3(NeuGc)‐liposomes belonged to CD4(‐)/CD8(‐)double‐negative CD3(+) CTL while GM3(NeuAc)‐liposomes induced two types of T cells, CD4(+) T cells and double‐negative I‐J positive T cells which mediated inhibition of the induction of anti‐melanoma CTL responses. These cell types were tbe same as those induced by mitomycin C‐treated melanoma cells for CTL induction and soluble melanoma antigen for Ts generation. The results clearly demonstrate that even GM3 with normal primary structure can, at a certain density, generate melanoma antlgenicity. |
format | Online Article Text |
id | pubmed-5917884 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 1989 |
publisher | Blackwell Publishing Ltd |
record_format | MEDLINE/PubMed |
spelling | pubmed-59178842018-05-11 Density of GM3 with Normal Primary Structure Determines Mouse Melanoma Antigenicity; a New Concept of Tumor Antigen Harada, Yoshitada Sakatsume, Minoru Nores, Gustavo A. Hakomori, Sen‐itiroh Taniguchi, Masaru Jpn J Cancer Res Article We attempted to induce anti‐melanoma cytotoxic T cells (CTL) and suppressor T cells (Ts) inhibiting CTL generation by using liposomes carrying various densities of GM3 as tumor antigens. We found that liposomes carrying 6–16 mol% of GM3 with normal primary structure successfully generated anti‐melanoma CTL and suppressor T cells, while liposomes with GM3 outside this range had little or no such activity. Anti‐melanoma CTL induced by GM3(NeuGc)‐liposomes belonged to CD4(‐)/CD8(‐)double‐negative CD3(+) CTL while GM3(NeuAc)‐liposomes induced two types of T cells, CD4(+) T cells and double‐negative I‐J positive T cells which mediated inhibition of the induction of anti‐melanoma CTL responses. These cell types were tbe same as those induced by mitomycin C‐treated melanoma cells for CTL induction and soluble melanoma antigen for Ts generation. The results clearly demonstrate that even GM3 with normal primary structure can, at a certain density, generate melanoma antlgenicity. Blackwell Publishing Ltd 1989-10 /pmc/articles/PMC5917884/ /pubmed/2533193 http://dx.doi.org/10.1111/j.1349-7006.1989.tb01638.x Text en |
spellingShingle | Article Harada, Yoshitada Sakatsume, Minoru Nores, Gustavo A. Hakomori, Sen‐itiroh Taniguchi, Masaru Density of GM3 with Normal Primary Structure Determines Mouse Melanoma Antigenicity; a New Concept of Tumor Antigen |
title | Density of GM3 with Normal Primary Structure Determines Mouse Melanoma Antigenicity; a New Concept of Tumor Antigen |
title_full | Density of GM3 with Normal Primary Structure Determines Mouse Melanoma Antigenicity; a New Concept of Tumor Antigen |
title_fullStr | Density of GM3 with Normal Primary Structure Determines Mouse Melanoma Antigenicity; a New Concept of Tumor Antigen |
title_full_unstemmed | Density of GM3 with Normal Primary Structure Determines Mouse Melanoma Antigenicity; a New Concept of Tumor Antigen |
title_short | Density of GM3 with Normal Primary Structure Determines Mouse Melanoma Antigenicity; a New Concept of Tumor Antigen |
title_sort | density of gm3 with normal primary structure determines mouse melanoma antigenicity; a new concept of tumor antigen |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5917884/ https://www.ncbi.nlm.nih.gov/pubmed/2533193 http://dx.doi.org/10.1111/j.1349-7006.1989.tb01638.x |
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