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A Variant Schmidt‐Ruppin Strain of Rous Sarcoma Virus with Increased Affinity for Mammalian Cells

SR‐RSV‐D(H), a variant virus with extremely high tropism for mammalian cells, was isolated by passage of the Schmidt‐Ruppin strain of Rous sarcoma virus of subgroup D (SR‐RSV‐D) through hamster cells. This variant virus has acquired an altered envelope glycoprotein, encoded by the env gene, that has...

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Autores principales: Kawai, Sadaaki, Nishizawa, Makoto, Shinno‐Kohno, Hideko, Shiroki, Kazuko
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Blackwell Publishing Ltd 1989
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5917936/
https://www.ncbi.nlm.nih.gov/pubmed/2560773
http://dx.doi.org/10.1111/j.1349-7006.1989.tb01652.x
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author Kawai, Sadaaki
Nishizawa, Makoto
Shinno‐Kohno, Hideko
Shiroki, Kazuko
author_facet Kawai, Sadaaki
Nishizawa, Makoto
Shinno‐Kohno, Hideko
Shiroki, Kazuko
author_sort Kawai, Sadaaki
collection PubMed
description SR‐RSV‐D(H), a variant virus with extremely high tropism for mammalian cells, was isolated by passage of the Schmidt‐Ruppin strain of Rous sarcoma virus of subgroup D (SR‐RSV‐D) through hamster cells. This variant virus has acquired an altered envelope glycoprotein, encoded by the env gene, that has high affinity for receptors on the surface of mammalian cells. The variant virus transforms rat cells at about 100 times the efficiency of the parental virus, SR‐RSV‐D(S), as assayed by focus formation. Addition of amphotericin B (Fungizone) to the medium at a concentration of 0.2 μg/ml completely inhibited rat cell transformation by SR‐RSV‐D(H), possibly by blocking virus penetration into the cells, whereas the drug showed no inhibitory effect on transformation of chick embryo flbroblast (CEF) cells by the variant virus or on transformation of rat cells by the parental virus. The efficiency of transformation of rat cells by the variant virus was much less than its efficiency of transformation of CEF cells. Analysis of infection of rat cells suggested that the virus can infect rat cells as efficiently as CEF cells but that rat cells were not transformed by the virus as fully as CEF cells because of inefficiency of some post‐penetrational step involved in viral gene expression. The finding that El AY cells, rat cells expressing adenovirus El A gene, were transformed by SR‐RSV‐D(H) as efficiently as CEF cells supports this conclusion and suggests that expression of the E1A gene in rat cells may overcome the defect in the transforming step(s) in rat cells.
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spelling pubmed-59179362018-05-11 A Variant Schmidt‐Ruppin Strain of Rous Sarcoma Virus with Increased Affinity for Mammalian Cells Kawai, Sadaaki Nishizawa, Makoto Shinno‐Kohno, Hideko Shiroki, Kazuko Jpn J Cancer Res Article SR‐RSV‐D(H), a variant virus with extremely high tropism for mammalian cells, was isolated by passage of the Schmidt‐Ruppin strain of Rous sarcoma virus of subgroup D (SR‐RSV‐D) through hamster cells. This variant virus has acquired an altered envelope glycoprotein, encoded by the env gene, that has high affinity for receptors on the surface of mammalian cells. The variant virus transforms rat cells at about 100 times the efficiency of the parental virus, SR‐RSV‐D(S), as assayed by focus formation. Addition of amphotericin B (Fungizone) to the medium at a concentration of 0.2 μg/ml completely inhibited rat cell transformation by SR‐RSV‐D(H), possibly by blocking virus penetration into the cells, whereas the drug showed no inhibitory effect on transformation of chick embryo flbroblast (CEF) cells by the variant virus or on transformation of rat cells by the parental virus. The efficiency of transformation of rat cells by the variant virus was much less than its efficiency of transformation of CEF cells. Analysis of infection of rat cells suggested that the virus can infect rat cells as efficiently as CEF cells but that rat cells were not transformed by the virus as fully as CEF cells because of inefficiency of some post‐penetrational step involved in viral gene expression. The finding that El AY cells, rat cells expressing adenovirus El A gene, were transformed by SR‐RSV‐D(H) as efficiently as CEF cells supports this conclusion and suggests that expression of the E1A gene in rat cells may overcome the defect in the transforming step(s) in rat cells. Blackwell Publishing Ltd 1989-12 /pmc/articles/PMC5917936/ /pubmed/2560773 http://dx.doi.org/10.1111/j.1349-7006.1989.tb01652.x Text en
spellingShingle Article
Kawai, Sadaaki
Nishizawa, Makoto
Shinno‐Kohno, Hideko
Shiroki, Kazuko
A Variant Schmidt‐Ruppin Strain of Rous Sarcoma Virus with Increased Affinity for Mammalian Cells
title A Variant Schmidt‐Ruppin Strain of Rous Sarcoma Virus with Increased Affinity for Mammalian Cells
title_full A Variant Schmidt‐Ruppin Strain of Rous Sarcoma Virus with Increased Affinity for Mammalian Cells
title_fullStr A Variant Schmidt‐Ruppin Strain of Rous Sarcoma Virus with Increased Affinity for Mammalian Cells
title_full_unstemmed A Variant Schmidt‐Ruppin Strain of Rous Sarcoma Virus with Increased Affinity for Mammalian Cells
title_short A Variant Schmidt‐Ruppin Strain of Rous Sarcoma Virus with Increased Affinity for Mammalian Cells
title_sort variant schmidt‐ruppin strain of rous sarcoma virus with increased affinity for mammalian cells
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5917936/
https://www.ncbi.nlm.nih.gov/pubmed/2560773
http://dx.doi.org/10.1111/j.1349-7006.1989.tb01652.x
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