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Amplification and Specific Detection of Transforming Gene Region of Human Papillomavirus 16, 18 and 33 in Cervical Carcinoma by Means of the Polymerase Chain Reaction
We have established a highly sensitive method for specific detection of human papillomavirus (HPV) 16, 18 and 33, by using the polymerase chain reaction (PCR). A HPV‐related sequence (140 bp) in the E6 transforming region was specifically amplified and detected by gel electrophoresis and by the use...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Blackwell Publishing Ltd
1990
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5917956/ https://www.ncbi.nlm.nih.gov/pubmed/2157691 http://dx.doi.org/10.1111/j.1349-7006.1990.tb02498.x |
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author | Shimada, Masamitsu Fukushima, Michio Mukai, Hiroyuki Kato, Ikunoshin Nishikawa, Akira Fujinaga, Kei |
author_facet | Shimada, Masamitsu Fukushima, Michio Mukai, Hiroyuki Kato, Ikunoshin Nishikawa, Akira Fujinaga, Kei |
author_sort | Shimada, Masamitsu |
collection | PubMed |
description | We have established a highly sensitive method for specific detection of human papillomavirus (HPV) 16, 18 and 33, by using the polymerase chain reaction (PCR). A HPV‐related sequence (140 bp) in the E6 transforming region was specifically amplified and detected by gel electrophoresis and by the use of a specific oligonucleotide probe. The PCR could detect 10(‐5)–10(‐6) copies per cell (maximum sensitivity). Furthermore, HPV 16, 18 and 33 DNAs were synthesized in a common reaction solution and specifically detected by HPV type‐specific probes. The PCR detected the HPV sequence from tissues which were negative to Southern hybridization. This detection technique may contribute significantly to the precise analysis of HPV in small proliferative lesions in the cervix. |
format | Online Article Text |
id | pubmed-5917956 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 1990 |
publisher | Blackwell Publishing Ltd |
record_format | MEDLINE/PubMed |
spelling | pubmed-59179562018-05-11 Amplification and Specific Detection of Transforming Gene Region of Human Papillomavirus 16, 18 and 33 in Cervical Carcinoma by Means of the Polymerase Chain Reaction Shimada, Masamitsu Fukushima, Michio Mukai, Hiroyuki Kato, Ikunoshin Nishikawa, Akira Fujinaga, Kei Jpn J Cancer Res Rapid Communication We have established a highly sensitive method for specific detection of human papillomavirus (HPV) 16, 18 and 33, by using the polymerase chain reaction (PCR). A HPV‐related sequence (140 bp) in the E6 transforming region was specifically amplified and detected by gel electrophoresis and by the use of a specific oligonucleotide probe. The PCR could detect 10(‐5)–10(‐6) copies per cell (maximum sensitivity). Furthermore, HPV 16, 18 and 33 DNAs were synthesized in a common reaction solution and specifically detected by HPV type‐specific probes. The PCR detected the HPV sequence from tissues which were negative to Southern hybridization. This detection technique may contribute significantly to the precise analysis of HPV in small proliferative lesions in the cervix. Blackwell Publishing Ltd 1990-01 /pmc/articles/PMC5917956/ /pubmed/2157691 http://dx.doi.org/10.1111/j.1349-7006.1990.tb02498.x Text en |
spellingShingle | Rapid Communication Shimada, Masamitsu Fukushima, Michio Mukai, Hiroyuki Kato, Ikunoshin Nishikawa, Akira Fujinaga, Kei Amplification and Specific Detection of Transforming Gene Region of Human Papillomavirus 16, 18 and 33 in Cervical Carcinoma by Means of the Polymerase Chain Reaction |
title | Amplification and Specific Detection of Transforming Gene Region of Human Papillomavirus 16, 18 and 33 in Cervical Carcinoma by Means of the Polymerase Chain Reaction |
title_full | Amplification and Specific Detection of Transforming Gene Region of Human Papillomavirus 16, 18 and 33 in Cervical Carcinoma by Means of the Polymerase Chain Reaction |
title_fullStr | Amplification and Specific Detection of Transforming Gene Region of Human Papillomavirus 16, 18 and 33 in Cervical Carcinoma by Means of the Polymerase Chain Reaction |
title_full_unstemmed | Amplification and Specific Detection of Transforming Gene Region of Human Papillomavirus 16, 18 and 33 in Cervical Carcinoma by Means of the Polymerase Chain Reaction |
title_short | Amplification and Specific Detection of Transforming Gene Region of Human Papillomavirus 16, 18 and 33 in Cervical Carcinoma by Means of the Polymerase Chain Reaction |
title_sort | amplification and specific detection of transforming gene region of human papillomavirus 16, 18 and 33 in cervical carcinoma by means of the polymerase chain reaction |
topic | Rapid Communication |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5917956/ https://www.ncbi.nlm.nih.gov/pubmed/2157691 http://dx.doi.org/10.1111/j.1349-7006.1990.tb02498.x |
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