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Amplification and Specific Detection of Transforming Gene Region of Human Papillomavirus 16, 18 and 33 in Cervical Carcinoma by Means of the Polymerase Chain Reaction

We have established a highly sensitive method for specific detection of human papillomavirus (HPV) 16, 18 and 33, by using the polymerase chain reaction (PCR). A HPV‐related sequence (140 bp) in the E6 transforming region was specifically amplified and detected by gel electrophoresis and by the use...

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Detalles Bibliográficos
Autores principales: Shimada, Masamitsu, Fukushima, Michio, Mukai, Hiroyuki, Kato, Ikunoshin, Nishikawa, Akira, Fujinaga, Kei
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Blackwell Publishing Ltd 1990
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5917956/
https://www.ncbi.nlm.nih.gov/pubmed/2157691
http://dx.doi.org/10.1111/j.1349-7006.1990.tb02498.x
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author Shimada, Masamitsu
Fukushima, Michio
Mukai, Hiroyuki
Kato, Ikunoshin
Nishikawa, Akira
Fujinaga, Kei
author_facet Shimada, Masamitsu
Fukushima, Michio
Mukai, Hiroyuki
Kato, Ikunoshin
Nishikawa, Akira
Fujinaga, Kei
author_sort Shimada, Masamitsu
collection PubMed
description We have established a highly sensitive method for specific detection of human papillomavirus (HPV) 16, 18 and 33, by using the polymerase chain reaction (PCR). A HPV‐related sequence (140 bp) in the E6 transforming region was specifically amplified and detected by gel electrophoresis and by the use of a specific oligonucleotide probe. The PCR could detect 10(‐5)–10(‐6) copies per cell (maximum sensitivity). Furthermore, HPV 16, 18 and 33 DNAs were synthesized in a common reaction solution and specifically detected by HPV type‐specific probes. The PCR detected the HPV sequence from tissues which were negative to Southern hybridization. This detection technique may contribute significantly to the precise analysis of HPV in small proliferative lesions in the cervix.
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spelling pubmed-59179562018-05-11 Amplification and Specific Detection of Transforming Gene Region of Human Papillomavirus 16, 18 and 33 in Cervical Carcinoma by Means of the Polymerase Chain Reaction Shimada, Masamitsu Fukushima, Michio Mukai, Hiroyuki Kato, Ikunoshin Nishikawa, Akira Fujinaga, Kei Jpn J Cancer Res Rapid Communication We have established a highly sensitive method for specific detection of human papillomavirus (HPV) 16, 18 and 33, by using the polymerase chain reaction (PCR). A HPV‐related sequence (140 bp) in the E6 transforming region was specifically amplified and detected by gel electrophoresis and by the use of a specific oligonucleotide probe. The PCR could detect 10(‐5)–10(‐6) copies per cell (maximum sensitivity). Furthermore, HPV 16, 18 and 33 DNAs were synthesized in a common reaction solution and specifically detected by HPV type‐specific probes. The PCR detected the HPV sequence from tissues which were negative to Southern hybridization. This detection technique may contribute significantly to the precise analysis of HPV in small proliferative lesions in the cervix. Blackwell Publishing Ltd 1990-01 /pmc/articles/PMC5917956/ /pubmed/2157691 http://dx.doi.org/10.1111/j.1349-7006.1990.tb02498.x Text en
spellingShingle Rapid Communication
Shimada, Masamitsu
Fukushima, Michio
Mukai, Hiroyuki
Kato, Ikunoshin
Nishikawa, Akira
Fujinaga, Kei
Amplification and Specific Detection of Transforming Gene Region of Human Papillomavirus 16, 18 and 33 in Cervical Carcinoma by Means of the Polymerase Chain Reaction
title Amplification and Specific Detection of Transforming Gene Region of Human Papillomavirus 16, 18 and 33 in Cervical Carcinoma by Means of the Polymerase Chain Reaction
title_full Amplification and Specific Detection of Transforming Gene Region of Human Papillomavirus 16, 18 and 33 in Cervical Carcinoma by Means of the Polymerase Chain Reaction
title_fullStr Amplification and Specific Detection of Transforming Gene Region of Human Papillomavirus 16, 18 and 33 in Cervical Carcinoma by Means of the Polymerase Chain Reaction
title_full_unstemmed Amplification and Specific Detection of Transforming Gene Region of Human Papillomavirus 16, 18 and 33 in Cervical Carcinoma by Means of the Polymerase Chain Reaction
title_short Amplification and Specific Detection of Transforming Gene Region of Human Papillomavirus 16, 18 and 33 in Cervical Carcinoma by Means of the Polymerase Chain Reaction
title_sort amplification and specific detection of transforming gene region of human papillomavirus 16, 18 and 33 in cervical carcinoma by means of the polymerase chain reaction
topic Rapid Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5917956/
https://www.ncbi.nlm.nih.gov/pubmed/2157691
http://dx.doi.org/10.1111/j.1349-7006.1990.tb02498.x
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