Cargando…

Prevalence of Human Papillomavirus Types 16 and 18 in Cervical Carcinomas: A Study by Dot and Southern Blot Hybridization and the Polymerase Chain Reaction

Histologically classified biopsies from 83 women with invasive cervical carcinoma were analyzed by dot blot hybridization for human papillomavirus (HPV) types 16 and 18 infection. Sixty of the 83 (72.3%) were found to contain HPV DNA, of which 43 (51.8%) contained HPV 16 DNA, 12 (14.5%) contained HP...

Descripción completa

Detalles Bibliográficos
Autores principales: Low, Seng‐Hui, Thong, Tuck‐Weng, Ho, Tew‐Hong, Lee, Yoke‐Sun, Morita, Takashi, Singh, Mulkit, Yap, Eu‐Hian, Chan, Yow‐Cheong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Blackwell Publishing Ltd 1990
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5917980/
https://www.ncbi.nlm.nih.gov/pubmed/2176202
http://dx.doi.org/10.1111/j.1349-7006.1990.tb02522.x
_version_ 1783317332189249536
author Low, Seng‐Hui
Thong, Tuck‐Weng
Ho, Tew‐Hong
Lee, Yoke‐Sun
Morita, Takashi
Singh, Mulkit
Yap, Eu‐Hian
Chan, Yow‐Cheong
author_facet Low, Seng‐Hui
Thong, Tuck‐Weng
Ho, Tew‐Hong
Lee, Yoke‐Sun
Morita, Takashi
Singh, Mulkit
Yap, Eu‐Hian
Chan, Yow‐Cheong
author_sort Low, Seng‐Hui
collection PubMed
description Histologically classified biopsies from 83 women with invasive cervical carcinoma were analyzed by dot blot hybridization for human papillomavirus (HPV) types 16 and 18 infection. Sixty of the 83 (72.3%) were found to contain HPV DNA, of which 43 (51.8%) contained HPV 16 DNA, 12 (14.5%) contained HPV 18 DNA and 5 (6.0%) contained both HPV 16 and 18 DNAs. Southern blot analysis on 65 specimens gave similar results. Of 23 specimens negative by dot blot, 21 were tested by the polymerase chain reaction. Seventeen of the 21 were positive for HPV DNA, of which 13 contained HPV 16 DNA and 4 contained both HPV 16 and 18 DNAs. In all, 95.1% (77/81) were positive for HPV 16 and/or 18 DNA sequences.
format Online
Article
Text
id pubmed-5917980
institution National Center for Biotechnology Information
language English
publishDate 1990
publisher Blackwell Publishing Ltd
record_format MEDLINE/PubMed
spelling pubmed-59179802018-05-11 Prevalence of Human Papillomavirus Types 16 and 18 in Cervical Carcinomas: A Study by Dot and Southern Blot Hybridization and the Polymerase Chain Reaction Low, Seng‐Hui Thong, Tuck‐Weng Ho, Tew‐Hong Lee, Yoke‐Sun Morita, Takashi Singh, Mulkit Yap, Eu‐Hian Chan, Yow‐Cheong Jpn J Cancer Res Article Histologically classified biopsies from 83 women with invasive cervical carcinoma were analyzed by dot blot hybridization for human papillomavirus (HPV) types 16 and 18 infection. Sixty of the 83 (72.3%) were found to contain HPV DNA, of which 43 (51.8%) contained HPV 16 DNA, 12 (14.5%) contained HPV 18 DNA and 5 (6.0%) contained both HPV 16 and 18 DNAs. Southern blot analysis on 65 specimens gave similar results. Of 23 specimens negative by dot blot, 21 were tested by the polymerase chain reaction. Seventeen of the 21 were positive for HPV DNA, of which 13 contained HPV 16 DNA and 4 contained both HPV 16 and 18 DNAs. In all, 95.1% (77/81) were positive for HPV 16 and/or 18 DNA sequences. Blackwell Publishing Ltd 1990-11 /pmc/articles/PMC5917980/ /pubmed/2176202 http://dx.doi.org/10.1111/j.1349-7006.1990.tb02522.x Text en
spellingShingle Article
Low, Seng‐Hui
Thong, Tuck‐Weng
Ho, Tew‐Hong
Lee, Yoke‐Sun
Morita, Takashi
Singh, Mulkit
Yap, Eu‐Hian
Chan, Yow‐Cheong
Prevalence of Human Papillomavirus Types 16 and 18 in Cervical Carcinomas: A Study by Dot and Southern Blot Hybridization and the Polymerase Chain Reaction
title Prevalence of Human Papillomavirus Types 16 and 18 in Cervical Carcinomas: A Study by Dot and Southern Blot Hybridization and the Polymerase Chain Reaction
title_full Prevalence of Human Papillomavirus Types 16 and 18 in Cervical Carcinomas: A Study by Dot and Southern Blot Hybridization and the Polymerase Chain Reaction
title_fullStr Prevalence of Human Papillomavirus Types 16 and 18 in Cervical Carcinomas: A Study by Dot and Southern Blot Hybridization and the Polymerase Chain Reaction
title_full_unstemmed Prevalence of Human Papillomavirus Types 16 and 18 in Cervical Carcinomas: A Study by Dot and Southern Blot Hybridization and the Polymerase Chain Reaction
title_short Prevalence of Human Papillomavirus Types 16 and 18 in Cervical Carcinomas: A Study by Dot and Southern Blot Hybridization and the Polymerase Chain Reaction
title_sort prevalence of human papillomavirus types 16 and 18 in cervical carcinomas: a study by dot and southern blot hybridization and the polymerase chain reaction
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5917980/
https://www.ncbi.nlm.nih.gov/pubmed/2176202
http://dx.doi.org/10.1111/j.1349-7006.1990.tb02522.x
work_keys_str_mv AT lowsenghui prevalenceofhumanpapillomavirustypes16and18incervicalcarcinomasastudybydotandsouthernblothybridizationandthepolymerasechainreaction
AT thongtuckweng prevalenceofhumanpapillomavirustypes16and18incervicalcarcinomasastudybydotandsouthernblothybridizationandthepolymerasechainreaction
AT hotewhong prevalenceofhumanpapillomavirustypes16and18incervicalcarcinomasastudybydotandsouthernblothybridizationandthepolymerasechainreaction
AT leeyokesun prevalenceofhumanpapillomavirustypes16and18incervicalcarcinomasastudybydotandsouthernblothybridizationandthepolymerasechainreaction
AT moritatakashi prevalenceofhumanpapillomavirustypes16and18incervicalcarcinomasastudybydotandsouthernblothybridizationandthepolymerasechainreaction
AT singhmulkit prevalenceofhumanpapillomavirustypes16and18incervicalcarcinomasastudybydotandsouthernblothybridizationandthepolymerasechainreaction
AT yapeuhian prevalenceofhumanpapillomavirustypes16and18incervicalcarcinomasastudybydotandsouthernblothybridizationandthepolymerasechainreaction
AT chanyowcheong prevalenceofhumanpapillomavirustypes16and18incervicalcarcinomasastudybydotandsouthernblothybridizationandthepolymerasechainreaction